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Real-time interactive optical micromanipulation of a mixture of high- and low-index particles

Peter John Rodrigo, Vincent Ricardo Daria, and Jesper Glückstad

Open Access Open Access

Abstract

We demonstrate real-time interactive optical micromanipulation of a colloidal mixture consisting of particles with both lower (nL<n0) and higher (nH>n0) refractive indices than that of the suspending medium (n0). Spherical high- and low-index particles are trapped in the transverse plane by an array of confining optical potentials created by trapping beams with top-hat and annular cross-sectional intensity profiles, respectively. The applied method offers extensive reconfigurability in the spatial distribution and individual geometry of the optical traps. We experimentally demonstrate this unique feature by simultaneously trapping and independently manipulating various sizes of spherical soda lime micro - shells (nL≈1.2) and polystyrene micro-beads (nH=1.57) suspended in water (n0=1.33).

©2004 Optical Society of America

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Supplementary Material (5)

Media 1: AVI (1656 KB)     
Media 2: AVI (1126 KB)     
Media 3: AVI (2512 KB)     
Media 4: AVI (1113 KB)     
Media 5: AVI (1518 KB)     

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Figures (8)
Fig. 1.
Fig. 1. Experimental setup for simultaneous optical manipulation of high - and low-index particles at the trapping plane. The expanded beam (λ=830 nm) incident at the spatial light modulator (SLM) comes from a CW Ti:Sapphire (Ti:S) laser pumped by a visible CW Nd:YVO4 laser. Under computer control, arbitrary 2D phase patterns are encoded onto the reflective SLM. A high -contrast intensity mapping of the phase pattern is formed at the image plane (IP) and is captured by a CCD camera via partial reflection from a pellicle. The intensity distribution is optically relayed to the trapping plane. Standard brightfield detection is used to observe the trapped particles. PCF: phase contrast filter, Ir: iris diaphragm, L1, L2 and L3: lenses, MO: microscope objective, DM: dichroic mirror, TL: tube lens.

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Fig. 2.
Fig. 2. (a) Measured high -contrast intensity pattern at the output plane IP. Corresponding surface intensity plots for the representative (b) top-hat (in yellow square) and (c) annular or doughnut (in green square) trapping beams.

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Fig. 3.
Fig. 3. Diagram of the optical potential (a) for a high-index (solid curve) and a low-index (dashed curve) particle due to a beam with top-hat transverse intensity profile, and (b) for a low-index particle due to a beam with annular transverse intensity profile.

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Fig. 4.
Fig. 4. (AVI, 1.656 MB) Deflection of a soda lime hollow glass sphere from a computer-mouse controlled trapping beam with top-hat intensity profile. An arrow in each frame indicates the location of the beam at that instant. Scale bar, 10 µm.

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Fig. 5.
Fig. 5. (AVI, 1.126 MB) Raking of low-index particles to a region of interest achieved by scanning a bright linear intensity pattern in the x-y plane. The arrow (frame 1) indicates the scanning direction. Scale bar, 10 µm.

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Fig. 6.
Fig. 6. (AVI, 2.512 MB) User-interactive procedure for trapping different sizes of hollow glass spheres using doughnut optical traps.

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Fig. 7.
Fig. 7. (AVI, 1.113 MB) Image sequences of trapping and user-interactive sorting of an inhomogeneous mixture of soda lime hollow glass spheres and polystyrene beads in water solution. (a) The particles are first captured by appropriate trapping beams and then (b–c) displaced one by one. The size of the beam used at each trapping site is proportional to the size of the corresponding particle. Arrows indicate the directions at which particles are transported. (d) Two separate rows of optically trapped high-index (lower row) and low-index particles (upper row). Scale bar, 10 µm.

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Fig. 8.
Fig. 8. (AVI, 1.518 MB) Simultaneously transported high- and low-index particles confined in respective optical traps with pre-programmed dynamics. The time interval between adjacent frames is ~15 s. Scale bar, 10 µm.

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