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Conferences > BODA > 2019 > DS2A > DS2A.2
  • Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)
  • OSA Technical Digest (Optica Publishing Group, 2019),
  • paper DS2A.2
  • •https://doi.org/10.1364/BODA.2019.DS2A.2

Comparison of Frozen Sections and Nonlinear Imaging for Evaluation of Mohs Surgical Margins

Michael G. Giacomelli, Tadayuki Yoshitake, Lucas Cahill, Beverly Faulkner-Jones, Daihung Do, and James Fujimoto

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Abstract

Rapid fluorescent labeling combined with optical sectioning and H&E-rendering algorithms can provide histological imaging of surgical specimens more rapidly than frozen sections by avoiding the need to freeze tissue and physically cut slides. Using a novel protocol that enables precise coregistration of nonlinear microscopy (NLM) and diagnostic frozen sections (FSA) produced during Mohs surgery, we present the results of a study comparing the relative accuracy of NLM and FSA.

© 2019 The Author(s)

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