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Overcoming the Fundamental Limit of Two-Photon Microscopy With Non-Degenerate Excitation

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Abstract

We use non-degenerate two-photon excitation where the two excitation beams are displaced in space outside the focal volume to increase the signal-to-background ratio (SBR), overcoming the fundamental penetration depth limit of conventional two-photon microscopy.

© 2020 The Author(s)

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