Abstract
The laser-based technique of single-particle aerodynamic relaxation time (SPART) analysis has been applied to the rapid detection and characterization of aerosolized bacteria. The automated SPART method subjects aerosol particles to a 24- kHz acoustic excitation field, monitors the resulting particle motion using a dual-beam frequency-biased laser Doppler velocimeter, and aerodynamically sizes the particles in real time by measuring the phase lag of the particle motion relative to the driving field. Nonviable rod-shaped bacteria Bacillus subtilis and Escherichia coli and the spherical bacterium Staphylococcus epidermidis were aerosolized using the conventional technique of pneumatic atomization. A U-Mid compressed air nebulizer proved effective in generating bacterial aerosols of both spherical and rod-shaped morphologies in the approximate concentration range of 104 particles/cm3.
© 1985 Optical Society of America
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