Abstract
The survival of photosynthetical organisms crucially depends on their ability to adapt to different light intensities. Under excess light conditions, the fastest photoprotection response consists in the turn-on of a dissipation mechanism of the energy absorbed by the chlorophylls into heat, called non-photochemical quenching (NPQ). NPQ requires the action of an intrathylakoidal protein, LHCSR3, composed of different chlorophylls and carotenoids bound to a tri-helicoidal carbon chain. During the light phase of photosynthesis, high light stress conditions generate a pH gradient across the thylakoid membrane which is sensed by the acid groups of LHCSR3 and triggers the protein quenching activity In this work, we reproduce the quenching of the LHCSR3 protein in vitro considering two different pH conditions and two protein aggregation states (HA: high aggregation, LA: low aggregation). NPQ was studied using a combination of picosecond time-resolved photoluminescence (TRPL) and femtosecond transient absorption (TA) techniques in the visible and NIR spectral regions.
© 2019 IEEE
PDF ArticleMore Like This
Sergiu Amarie, Andreas Dreuw, Josef Wachtveitl, Tiago Barros, Jörg Standfuss, and Werner Kühlbrandt
MH11 International Conference on Ultrafast Phenomena (UP) 2006
G. Cerullo, D. Polli, G. Lanzani, S. De Silvestri, R.J. Cogdell, and H. Hashimoto
WD39 International Conference on Ultrafast Phenomena (UP) 2002
Daniela Serien and Koji Sugioka
20a_E214_2 JSAP-OSA Joint Symposia (JSAP) 2019