Second Harmonic Microscopy of Collagen

Rebecca M. Williams; Warren R. Zipfel; Watt W. Webb

doi:10.1364/FIO.2003.ThEE1

Frontiers in Optics
OSA Technical Digest (CD) (Optica Publishing Group, 2003),
paper ThEE1
•https://doi.org/10.1364/FIO.2003.ThEE1

Second Harmonic Microscopy of Collagen

Rebecca M. Williams, Warren R. Zipfel, and Watt W. Webb

Frontiers in Optics 2003

Tucson, Arizona United States
5–8 October 2003
ISBN: 1-55752-759-8

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Second Harmonic Imaging (ThEE)

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R. M. Williams, W. R. Zipfel, and W. W. Webb, "Second Harmonic Microscopy of Collagen," in Frontiers in Optics, OSA Technical Digest (CD) (Optica Publishing Group, 2003), paper ThEE1.

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Abstract

Second harmonic generation in conjunction with laser scanning microscopy can be used to produce detailed images of collagen structure in tissue explants or even living animals. Fundamental principles that govern the directionality of coherent emission from a tightly focused beam can yield sub-resolution information when interpreting collagen images.

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