We have quantified the influence of light scattering on confocal fluorescence anisotropy images at depth in turbid media. The artificial reduction in anisotropy at depth is explored by changing scattering particle sizes, depth of image acquisition, and microscope imaging parameters. Results from model systems are compared with Monte Carlo simulations.

© 2004 Optical Society of America

PDF Article


You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
Login to access OSA Member Subscription