Multiphoton histology of entire intact mouse organs

Sonia Parra; Joseph P. Zinter; Michael J. Levene

doi:10.1364/FIO.2009.FWA5

Frontiers in Optics 2009/Laser Science XXV/Fall 2009 OSA Optics & Photonics Technical Digest
OSA Technical Digest (CD) (Optica Publishing Group, 2009),
paper FWA5
•https://doi.org/10.1364/FIO.2009.FWA5

Multiphoton histology of entire intact mouse organs

Sonia Parra, Joseph P. Zinter, and Michael J. Levene

Frontiers in Optics 2009

San Jose, California United States
11–15 October 2009
ISBN: 978-1-55752-878-0

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Biomedical Applications of Ultrafast Lasers (FWA)

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S. Parra, J. P. Zinter, and M. J. Levene, "Multiphoton histology of entire intact mouse organs," in Frontiers in Optics 2009/Laser Science XXV/Fall 2009 OSA Optics & Photonics Technical Digest, OSA Technical Digest (CD) (Optica Publishing Group, 2009), paper FWA5.

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Abstract

We present multiphoton fluorescence microscopy and second harmonic imaging of entire intact, fixed and optically cleared mouse organs. We achieved imaging depths of several millimeters in mouse intestine, heart, lung, brain and other organs.

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