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Experimental verification of multispectral forward scatter phenotyping from bacterial colonies

Huisung Kim, Iyll-Joon Doh, Galen B. King, Arun K. Bhunia, and Euiwon Bae

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Abstract

We present an experimental verification of multispectral forward scattering from individual bacteria colonies. A new instrument was designed with a pellicle beam splitter, optical cage system, and three diode lasers (405 nm, 635 nm and 904 nm) to provide broader spectral data from bacterial colonies. Experiments with Staphylococcus colonies show excellent agreement with theories.

© 2014 Optical Society of America

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