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Conferences > ISA > 2019 > ITh1C > ITh1C.4

Quasi-noise-free stimulated emission depletion microscopy imaging of thick samples using adaptive optics and block-matching 3D filtering

Piotr Zdankowski, Maciej Trusiak, David McGloin, and Jason R. Swedlow

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Abstract

We present a novel-method of increasing signal-to-noise-ratio and effective-resolution of STED microscope by combining aberration-correction and image-processing. We imaged 15μm thick mitotic cell and observed in-plane resolution of 118nm without filtering and 70nm with filtering.

© 2019 The Author(s)

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