To gain a basic understanding of biological and chemical processes the acquisition of unaltered information from a molecular level is crucial. It can be achieved by Raman spectroscopy. However, extracting relevant information from Raman signals of complex multi-component systems is demanding, but decisive to correctly explain the observed phenomena. Two example applications are discussed: Raman spectroscopy for analysing hydrogen bonding in binary solutions; and shifted-excitation Raman difference spectroscopy (SERDS) for concentration measurements in a bioreactor.
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