Abstract
Measuring three-dimensional nanoscale cellular structures is challenging, especially when the structure is dynamic. Owing to the informative total internal reflection fluorescence (TIRF) imaging under varied illumination angles, multi-angle (MA) TIRF has been examined to offer a nanoscale axial and a subsecond temporal resolution. However, conventional MA-TIRF still performs badly in lateral resolution and fails to characterize the depth image in densely distributed regions. Here, we emphasize the lateral super-resolution in the MA-TIRF, exampled by simply introducing polarization modulation into the illumination procedure. Equipped with a sparsity and accelerated proximal algorithm, we examine a more precise 3D sample structure compared with previous methods, enabling live cell imaging with a temporal resolution of 2 s and recovering high-resolution mitochondria fission and fusion processes. We also shared the recovery program, which is the first open-source recovery code for MA-TIRF, to the best of our knowledge.
© 2018 Optical Society of America
Full Article | PDF ArticleMore Like This
Wenjie Liu, Qiulan Liu, Zhimin Zhang, Yubing Han, Cuifang Kuang, Liang Xu, Hongqin Yang, and Xu Liu
Opt. Express 27(5) 7237-7248 (2019)
Weijian Zong, Xiaoshuai Huang, Chi Zhang, Tianyi Yuan, Ling-ling Zhu, Ming Fan, and Liangyi Chen
Biomed. Opt. Express 5(5) 1530-1540 (2014)
Xiangyu Quan, Manoj Kumar, Osamu Matoba, Yasuhiro Awatsuji, Yoshio Hayasaki, Satoshi Hasegawa, and Hiroaki Wake
Opt. Lett. 43(21) 5447-5450 (2018)