Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Reverse contrast laser Doppler holography for lower frame rate retinal and choroidal blood flow imaging

Not Accessible

Your library or personal account may give you access

Abstract

Laser Doppler holography (LDH) is an interferometric blood flow imaging technique based on full-field measurements of the Doppler spectrum. LDH has so far been demonstrated in the retina with ultrafast cameras, typically at 75 kHz. We show here that a similar method can be implemented with camera frame rates 10 times slower than before. Due to energy conservation, low and high frequency local power Doppler signals have opposite variations, and a simple contrast inversion of the low frequency power Doppler reveals fast blood flow beyond the camera detection bandwidth for conventional laser Doppler measurements. Relevant blood flow variations and color composite power Doppler images can be obtained with camera frame rates down to a few kHz.

© 2020 Optical Society of America

Full Article  |  PDF Article
More Like This
Spatio-temporal filtering in laser Doppler holography for retinal blood flow imaging

Léo Puyo, Michel Paques, and Michael Atlan
Biomed. Opt. Express 11(6) 3274-3287 (2020)

Retinal blood flow imaging with combined full-field swept-source optical coherence tomography and laser Doppler holography

Léo Puyo, Hendrik Spahr, Clara Pfäffle, Gereon Hüttmann, and Dierck Hillmann
Opt. Lett. 47(5) 1198-1201 (2022)

Waveform analysis of human retinal and choroidal blood flow with laser Doppler holography

Léo Puyo, Michel Paques, Mathias Fink, José-Alain Sahel, and Michael Atlan
Biomed. Opt. Express 10(10) 4942-4963 (2019)

Supplementary Material (7)

NameDescription
Visualization 1       Doppler spectral response of the retina: the short-time window was averaged over an entire stack, measured at 67 kHz by laser Doppler holography. Normalized power Doppler images are shown for different frequency ranges.
Visualization 2       Retinal blood flow measured by LDH using the low and high frequency ranges.
Visualization 3       Retinal blood flow measured by low frame rate RC-LDH.
Visualization 4       Color composite movie of retinal blood flow measured by fast LDH. The power Doppler on the frequency ranges 1-6 and 6-33 kHz are fused in red and cyan to reveal fast and slow flow, respectively.
Visualization 5       Color composite movie of retinal blood flow measured by RC-LDH operating at 8 kHz. The power Doppler on the frequency ranges 0-1 (reversed contrast) and 1-4 kHz are fused in red and cyan to reveal fast and slow flow, respectively.
Visualization 6       Retinal blood flow movies measured by RC-LDH at 4 kHz.
Visualization 7       Retinal blood flow in a case of vascular impairment following a radiation therapy is imaged with RC-LDh operating at 10 kHz.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved