Abstract

Reproducibility and sensitivity of polarized light scattering measurements are explored for a particular rodlike bacterial species in different growth conditions. The angle dependence of certain Mueller scattering matrix elements (primarily S34/S11) is used to measure these features. The angular S34/S11 scattering pattern was found to be strongly correlated with bacterial cell size. In view of the large changes found for different growth conditions, it is concluded that much improvement in the technique of recognizing scattering patterns would be needed before the angle dependence of a given Mueller matrix element can be unequivocally associated with a given bacterial strain. The sensitivity of S34/S11 patterns (at a wavelength of 633 nm) to changes in internal structure of the bacteria was tested by following the pattern after virus particles were produced inside the individual bacteria by an induction procedure. No appreciable changes occurred up until the time the bacteria were lysed (destroyed) by the process.

© 1989 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Rapid optically based measurements of diameter and length for spherical or rod-shaped bacteria in vivo

Willem P. Van De Merwe, József Czégé, Merrill E. Milham, and Burt V. Bronk
Appl. Opt. 43(28) 5295-5302 (2004)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription