Abstract

The formula for the determination of a fluorescence polarization degree is derived for triple-chromophore complexes with energy transfer: P = (3B − 1 + 2A)/(3 + B + 4A). In this formula B = q 12 cos212) + q 13 cos213) + q 23 cos223) and parameters A and q ij are dependent on the kinetic parameters of energy exchange and the chromophore spectroscopic parameters. The angle ϑij is the angle between the transition dipole moments of the i and j chromophores. The formula was tested for the particular case of the C-phycocyanin (C-PC) of the blue-green algae Agmenellum quadruplicatum, which contain three chromophores, α-84, β-84, and β-155. Polarized spectra were calculated for both the emission and the excitation spectra of C-PC β subunits and monomers.

© 1994 Optical Society of America

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