Abstract

We studied the angular distribution of fluorescence from a small, lossy capillary filled with a laser-dye solution. We found that the fluorescence is isotropic for the liquid core and that, far from the liquid–solid phase transition, this isotropy shows no temperature dependence. This result, an extension of studies with solid cylinders, is at variance with theoretical expectations for solids as well as with previous reports by other investigators but is explained by the motion of the molecules in the liquid. Therefore the optimal viewing angle for capillary zone electrophoresis experiments is near 90° because the elastic scattering of the incident laser light is at or near a minimum for these small capillaries. This reduces contamination of the fluorescence signal as a result of stray laser light in the optical system.

© 1994 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Geometry-induced fluorescence resonances in small lossy capillaries

Boyd V. Hunter and William S. Bickel
Appl. Opt. 34(21) 4240-4247 (1995)

Fluorescent angular scattering emissions from dye-filled fibers

David Abromson and William S. Bickel
Appl. Opt. 30(21) 2980-2984 (1991)

Angular distribution of laser-induced fluorescence emission of active dyes in scattering media

Ali Bavali, Parviz Parvin, Mohammad Tavassoli, and Mohammad Reza Mohebbifar
Appl. Opt. 57(7) B32-B38 (2018)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (7)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription