Abstract

The maximum-likelihood criterion is shown to be a powerful method for analyzing fluorescence-detection data with small signal-to-noise ratios. A probability study of the maximum-likelihood criterion for a supposed single-molecule detection experiment is presented that takes into account the photokinetics of the molecule to be detected, its diffusion, and the laser-beam geometry. Furthermore, the efficiency of time-integrated and time-correlated single-photon counting methods are studied and compared.

© 1995 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Detection and identification of single molecules in solution

Steven A. Soper, Lloyd M. Davis, and E. Brooks Shera
J. Opt. Soc. Am. B 9(10) 1761-1769 (1992)

Rapid and efficient detection of single chromophore molecules in aqueous solution

Li-Qiang Li and Lloyd M. Davis
Appl. Opt. 34(18) 3208-3217 (1995)

Minimizing detection errors in single molecule localization microscopy

Pavel Křížek, Ivan Raška, and Guy M. Hagen
Opt. Express 19(4) 3226-3235 (2011)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (14)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (55)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription