Abstract

When attempting to integrate single-molecule fluorescence microscopy with microfabricated devices such as microfluidic channels, fabrication constraints may prevent using traditional coverslips. Instead, the fabricated devices may require imaging through material with a different thickness or index of refraction. Altering either can easily reduce the quality of the image formation (measured by the Strehl ratio) by a factor of 2 or more, reducing the signal-to-noise ratio accordingly. In such cases, successful detection of single-molecule fluorescence may prove difficult or impossible. Here we provide software to calculate the effect of non-design materials upon the Strehl ratio or ensquared energy and explore the impact of common materials used in microfabrication.

© 2019 Optical Society of America

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Supplementary Material (1)

NameDescription
» Code 1       This software is designed to calculate the effect of non-design microscope coverslip materials upon the Strehl ratio or ensquared energy using the Gibson-Lanni point spread function (PSF) model.

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