Abstract

Stimulated emission depletion (STED) nanoscopy is one of a suite of modern optical microscopy techniques capable of bypassing the conventional diffraction limit in fluorescent imaging. STED makes use of a spiral phase mask to enable 2D super-resolution imaging whereas to achieve full volumetric 3D super-resolution an additional bottle-beam phase mask must be applied. The resolution achieved in biological samples 10 µm or thicker is limited by aberrations induced mainly by scattering due to refractive index heterogeneity in the sample. These aberrations impact the fidelity of both types of phase mask, and have limited the application of STED to thicker biological systems. Here we apply an automated adaptive optics solution to correct the performance of both STED masks, enhancing robustness and expanding the capabilities of this nanoscopic technique. Corroboration in terms of successful high-quality imaging of the full volume of a 15µm mitotic spindle with resolution of 50nm x 50nm x 150nm achieved in all three dimensions is presented.

© 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

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2018 (2)

J. Heine, C. A. Wurm, J. Keller-Findeisen, A. Schönle, B. Harke, M. Reuss, F. R. Winter, and G. Donnert, “Three dimensional live-cell STED microscopy at increased depth using a water immersion objective,” Rev. Sci. Instrum. 89(5), 053701 (2018).
[Crossref] [PubMed]

C. Li, S. Liu, W. Wang, W. Liu, C. Kuang, and X. Liu, “Recent research on stimulated emission depletion microscopy for reducing photobleaching,” J. Microsc. 271(1), 4–16 (2018).
[Crossref] [PubMed]

2017 (6)

J. Antonello, D. Burke, and M. J. Booth, “Aberrations in stimulated emission depletion (STED) microscopy,” Opt. Commun. 404, 203–209 (2017).
[Crossref] [PubMed]

X. Chen, J. Chen, S. Dong, H. Yang, and S. Xie, “Effects of Seidel aberration and light polarization on the resolution of STED imaging,” Optik (Stuttg.) 130, 76–81 (2017).
[Crossref]

N. Ji, “Adaptive optical fluorescence microscopy,” Nat. Methods 14(4), 374–380 (2017).
[Crossref] [PubMed]

H. Blom and J. Widengren, “Stimulated Emission Depletion Microscopy,” Chem. Rev. 117(11), 7377–7427 (2017).
[Crossref] [PubMed]

W. Yan, Y. Yang, Y. Tan, X. Chen, Y. Li, J. Qu, and T. Ye, “Coherent optical adaptive technique improves the spatial resolution of STED microscopy in thick samples,” Photon. Res. 5(3), 176–181 (2017).
[Crossref] [PubMed]

A. Shihavuddin, S. Basu, E. Rexhepaj, F. Delestro, N. Menezes, S. M. Sigoillot, E. Del Nery, F. Selimi, N. Spassky, and A. Genovesio, “Smooth 2D manifold extraction from 3D image stack,” Nat. Commun. 8, 15554 (2017).
[Crossref] [PubMed]

2016 (3)

W. Yu, Z. Ji, D. Dong, X. Yang, Y. Xiao, Q. Gong, P. Xi, and K. Shi, “Super-resolution deep imaging with hollow Bessel beam STED microscopy,” Laser Photonics Rev. 10(1), 147–152 (2016).
[Crossref]

U. Böhm, S. W. Hell, and R. Schmidt, “4Pi-RESOLFT nanoscopy,” Nat. Commun. 7(1), 10504 (2016).
[Crossref] [PubMed]

B. R. Patton, D. Burke, D. Owald, T. J. Gould, J. Bewersdorf, and M. J. Booth, “Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics,” Opt. Express 24(8), 8862–8876 (2016).
[Crossref] [PubMed]

2015 (5)

Y. Wu, X. Wu, R. Lu, J. Zhang, L. Toro, and E. Stefani, “Resonant Scanning with Large Field of View Reduces Photobleaching and Enhances Fluorescence Yield in STED Microscopy,” Sci. Rep. 5, 14766 (2015).
[Crossref] [PubMed]

X. Wu, L. Toro, E. Stefani, and Y. Wu, “Ultrafast photon counting applied to resonant scanning STED microscopy,” J. Microsc. 257(1), 31–38 (2015).
[Crossref] [PubMed]

B. R. Patton, D. Burke, R. Vrees, and M. J. Booth, “Is phase-mask alignment aberrating your STED microscope?” Methods Appl. Fluoresc. 3(2), 024002 (2015).
[Crossref] [PubMed]

F. Curdt, S. J. Herr, T. Lutz, R. Schmidt, J. Engelhardt, S. J. Sahl, and S. W. Hell, “isoSTED nanoscopy with intrinsic beam alignment,” Opt. Express 23(24), 30891–30903 (2015).
[Crossref] [PubMed]

I. M. Vellekoop, “Feedback-based wavefront shaping,” Opt. Express 23(9), 12189–12206 (2015).
[Crossref] [PubMed]

2014 (2)

M. J. Booth, “Adaptive optical microscopy: The ongoing quest for a perfect image,” Light Sci. Appl. 3(4), e165 (2014).
[Crossref]

M. O. Lenz, H. G. Sinclair, A. Savell, J. H. Clegg, A. C. N. Brown, D. M. Davis, C. Dunsby, M. A. A. Neil, and P. M. W. French, “3-D stimulated emission depletion microscopy with programmable aberration correction,” J. Biophotonics 7(1-2), 29–36 (2014).
[Crossref] [PubMed]

2013 (3)

K. T. Takasaki, J. B. Ding, and B. L. Sabatini, “Live-cell superresolution imaging by pulsed STED two-photon excitation microscopy,” Biophys. J. 104(4), 770–777 (2013).
[Crossref] [PubMed]

P. Bethge, R. Chéreau, E. Avignone, G. Marsicano, and U. V. Nägerl, “Two-photon excitation STED microscopy in two colors in acute brain slices,” Biophys. J. 104(4), 778–785 (2013).
[Crossref] [PubMed]

F. Göttfert, C. A. Wurm, V. Mueller, S. Berning, V. C. Cordes, A. Honigmann, and S. W. Hell, “Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution,” Biophys. J. 105(1), L01–L03 (2013).
[Crossref] [PubMed]

2012 (4)

D. Wildanger, B. R. Patton, H. Schill, L. Marseglia, J. P. Hadden, S. Knauer, A. Schönle, J. G. Rarity, J. L. O’Brien, S. W. Hell, and J. M. Smith, “Solid immersion facilitates fluorescence microscopy with nanometer resolution and sub-ångström emitter localization,” Adv. Mater. 24(44), OP309–OP313 (2012).
[Crossref] [PubMed]

T. J. Gould, D. Burke, J. Bewersdorf, and M. J. Booth, “Adaptive optics enables 3D STED microscopy in aberrating specimens,” Opt. Express 20(19), 20998–21009 (2012).
[Crossref] [PubMed]

S. Galiani, B. Harke, G. Vicidomini, G. Lignani, F. Benfenati, A. Diaspro, and P. Bianchini, “Strategies to maximize the performance of a STED microscope,” Opt. Express 20(7), 7362–7374 (2012).
[Crossref] [PubMed]

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

2011 (1)

N. T. Urban, K. I. Willig, S. W. Hell, and U. V. Nägerl, “STED nanoscopy of actin dynamics in synapses deep inside living brain slices,” Biophys. J. 101(5), 1277–1284 (2011).
[Crossref] [PubMed]

2010 (2)

S. Deng, L. Liu, Y. Cheng, R. Li, and Z. Xu, “Effects of primary aberrations on the fluorescence depletion patterns of STED microscopy,” Opt. Express 18(2), 1657–1666 (2010).
[Crossref] [PubMed]

M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
[Crossref] [PubMed]

2009 (3)

S. Deng, L. Liu, Y. Cheng, R. Li, and Z. Xu, “Investigation of the influence of the aberration induced by a plane interface on STED microscopy,” Opt. Express 17(3), 1714–1725 (2009).
[Crossref] [PubMed]

D. Wildanger, R. Medda, L. Kastrup, and S. W. Hell, “A compact STED microscope providing 3D nanoscale resolution,” J. Microsc. 236(1), 35–43 (2009).
[Crossref] [PubMed]

E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, and S. W. Hell, “STED microscopy reveals crystal colour centres with nanometric resolution,” Nat. Photonics 3(3), 144–147 (2009).
[Crossref]

2008 (2)

2007 (2)

2004 (1)

2003 (1)

2002 (2)

M. J. Booth, M. A. A. Neil, R. Juskaitis, and T. Wilson, “Adaptive aberration correction in a confocal microscope,” Proc. Natl. Acad. Sci. U.S.A. 99(9), 5788–5792 (2002).
[Crossref] [PubMed]

S. A. Kennedy, M. J. Szabo, H. Teslow, J. Z. Porterfield, and E. R. I. Abraham, “Creation of Laguerre-Gaussian laser modes using diffractive optics,” Phys. Rev. A – At. Mol. Opt. Phys. 66, 438011 (2002).

2000 (2)

J. Arlt and M. J. Padgett, “Generation of a beam with a dark focus surrounded by regions of higher intensity: the optical bottle beam,” Opt. Lett. 25(4), 191–193 (2000).
[Crossref] [PubMed]

T. A. Klar, S. Jakobs, M. Dyba, A. Egner, and S. W. Hell, “Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission,” Proc. Natl. Acad. Sci. U.S.A. 97(15), 8206–8210 (2000).
[Crossref] [PubMed]

Abraham, E. R. I.

S. A. Kennedy, M. J. Szabo, H. Teslow, J. Z. Porterfield, and E. R. I. Abraham, “Creation of Laguerre-Gaussian laser modes using diffractive optics,” Phys. Rev. A – At. Mol. Opt. Phys. 66, 438011 (2002).

Allan, C.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
[Crossref] [PubMed]

Anguelov, D.

C. Szegedy, W. Liu, Y. Jia, P. Sermanet, S. Reed, D. Anguelov, D. Erhan, V. Vanhoucke, and A. Rabinovich, “Going deeper with convolutions,” in 2015 IEEE Conference on Computer Vision and Pattern Recognition (CVPR) (IEEE, 2015), 39, pp. 1–9.

Antonello, J.

J. Antonello, D. Burke, and M. J. Booth, “Aberrations in stimulated emission depletion (STED) microscopy,” Opt. Commun. 404, 203–209 (2017).
[Crossref] [PubMed]

Arlt, J.

Auksorius, E.

Avignone, E.

P. Bethge, R. Chéreau, E. Avignone, G. Marsicano, and U. V. Nägerl, “Two-photon excitation STED microscopy in two colors in acute brain slices,” Biophys. J. 104(4), 778–785 (2013).
[Crossref] [PubMed]

Avondo, J.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

Basu, S.

A. Shihavuddin, S. Basu, E. Rexhepaj, F. Delestro, N. Menezes, S. M. Sigoillot, E. Del Nery, F. Selimi, N. Spassky, and A. Genovesio, “Smooth 2D manifold extraction from 3D image stack,” Nat. Commun. 8, 15554 (2017).
[Crossref] [PubMed]

S. Basu, E. Rexhepaj, N. Spassky, A. Genovesio, R. Reinhold, and I. Curie, “FastSME : faster and smoother manifold extraction from 3D stack,” in Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition Workshops (2018).
[Crossref]

Benfenati, F.

Berning, S.

F. Göttfert, C. A. Wurm, V. Mueller, S. Berning, V. C. Cordes, A. Honigmann, and S. W. Hell, “Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution,” Biophys. J. 105(1), L01–L03 (2013).
[Crossref] [PubMed]

Best, C.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

Bethge, P.

P. Bethge, R. Chéreau, E. Avignone, G. Marsicano, and U. V. Nägerl, “Two-photon excitation STED microscopy in two colors in acute brain slices,” Biophys. J. 104(4), 778–785 (2013).
[Crossref] [PubMed]

Bewersdorf, J.

Bianchini, P.

Blackburn, C.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

Blom, H.

H. Blom and J. Widengren, “Stimulated Emission Depletion Microscopy,” Chem. Rev. 117(11), 7377–7427 (2017).
[Crossref] [PubMed]

Böhm, U.

U. Böhm, S. W. Hell, and R. Schmidt, “4Pi-RESOLFT nanoscopy,” Nat. Commun. 7(1), 10504 (2016).
[Crossref] [PubMed]

Booth, M. J.

J. Antonello, D. Burke, and M. J. Booth, “Aberrations in stimulated emission depletion (STED) microscopy,” Opt. Commun. 404, 203–209 (2017).
[Crossref] [PubMed]

B. R. Patton, D. Burke, D. Owald, T. J. Gould, J. Bewersdorf, and M. J. Booth, “Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics,” Opt. Express 24(8), 8862–8876 (2016).
[Crossref] [PubMed]

B. R. Patton, D. Burke, R. Vrees, and M. J. Booth, “Is phase-mask alignment aberrating your STED microscope?” Methods Appl. Fluoresc. 3(2), 024002 (2015).
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M. J. Booth, “Adaptive optical microscopy: The ongoing quest for a perfect image,” Light Sci. Appl. 3(4), e165 (2014).
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T. J. Gould, D. Burke, J. Bewersdorf, and M. J. Booth, “Adaptive optics enables 3D STED microscopy in aberrating specimens,” Opt. Express 20(19), 20998–21009 (2012).
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D. Debarre, M. J. Booth, and T. Wilson, “Image based adaptive optics through optimisation of low spatial frequencies,” Opt. Express 15(13), 8176–8190 (2007).
[Crossref] [PubMed]

M. J. Booth, M. A. A. Neil, R. Juskaitis, and T. Wilson, “Adaptive aberration correction in a confocal microscope,” Proc. Natl. Acad. Sci. U.S.A. 99(9), 5788–5792 (2002).
[Crossref] [PubMed]

Boruah, B. R.

Brown, A. C. N.

M. O. Lenz, H. G. Sinclair, A. Savell, J. H. Clegg, A. C. N. Brown, D. M. Davis, C. Dunsby, M. A. A. Neil, and P. M. W. French, “3-D stimulated emission depletion microscopy with programmable aberration correction,” J. Biophotonics 7(1-2), 29–36 (2014).
[Crossref] [PubMed]

Burel, J. M.

M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
[Crossref] [PubMed]

Burel, J.-M.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

Burke, D.

J. Antonello, D. Burke, and M. J. Booth, “Aberrations in stimulated emission depletion (STED) microscopy,” Opt. Commun. 404, 203–209 (2017).
[Crossref] [PubMed]

B. R. Patton, D. Burke, D. Owald, T. J. Gould, J. Bewersdorf, and M. J. Booth, “Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics,” Opt. Express 24(8), 8862–8876 (2016).
[Crossref] [PubMed]

B. R. Patton, D. Burke, R. Vrees, and M. J. Booth, “Is phase-mask alignment aberrating your STED microscope?” Methods Appl. Fluoresc. 3(2), 024002 (2015).
[Crossref] [PubMed]

T. J. Gould, D. Burke, J. Bewersdorf, and M. J. Booth, “Adaptive optics enables 3D STED microscopy in aberrating specimens,” Opt. Express 20(19), 20998–21009 (2012).
[Crossref] [PubMed]

Chen, J.

X. Chen, J. Chen, S. Dong, H. Yang, and S. Xie, “Effects of Seidel aberration and light polarization on the resolution of STED imaging,” Optik (Stuttg.) 130, 76–81 (2017).
[Crossref]

Chen, X.

X. Chen, J. Chen, S. Dong, H. Yang, and S. Xie, “Effects of Seidel aberration and light polarization on the resolution of STED imaging,” Optik (Stuttg.) 130, 76–81 (2017).
[Crossref]

W. Yan, Y. Yang, Y. Tan, X. Chen, Y. Li, J. Qu, and T. Ye, “Coherent optical adaptive technique improves the spatial resolution of STED microscopy in thick samples,” Photon. Res. 5(3), 176–181 (2017).
[Crossref] [PubMed]

Cheng, Y.

Chéreau, R.

P. Bethge, R. Chéreau, E. Avignone, G. Marsicano, and U. V. Nägerl, “Two-photon excitation STED microscopy in two colors in acute brain slices,” Biophys. J. 104(4), 778–785 (2013).
[Crossref] [PubMed]

Clegg, J. H.

M. O. Lenz, H. G. Sinclair, A. Savell, J. H. Clegg, A. C. N. Brown, D. M. Davis, C. Dunsby, M. A. A. Neil, and P. M. W. French, “3-D stimulated emission depletion microscopy with programmable aberration correction,” J. Biophotonics 7(1-2), 29–36 (2014).
[Crossref] [PubMed]

Cordes, V. C.

F. Göttfert, C. A. Wurm, V. Mueller, S. Berning, V. C. Cordes, A. Honigmann, and S. W. Hell, “Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution,” Biophys. J. 105(1), L01–L03 (2013).
[Crossref] [PubMed]

Curdt, F.

Curie, I.

S. Basu, E. Rexhepaj, N. Spassky, A. Genovesio, R. Reinhold, and I. Curie, “FastSME : faster and smoother manifold extraction from 3D stack,” in Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition Workshops (2018).
[Crossref]

Davis, D. M.

M. O. Lenz, H. G. Sinclair, A. Savell, J. H. Clegg, A. C. N. Brown, D. M. Davis, C. Dunsby, M. A. A. Neil, and P. M. W. French, “3-D stimulated emission depletion microscopy with programmable aberration correction,” J. Biophotonics 7(1-2), 29–36 (2014).
[Crossref] [PubMed]

Debarre, D.

Del Nery, E.

A. Shihavuddin, S. Basu, E. Rexhepaj, F. Delestro, N. Menezes, S. M. Sigoillot, E. Del Nery, F. Selimi, N. Spassky, and A. Genovesio, “Smooth 2D manifold extraction from 3D image stack,” Nat. Commun. 8, 15554 (2017).
[Crossref] [PubMed]

Delestro, F.

A. Shihavuddin, S. Basu, E. Rexhepaj, F. Delestro, N. Menezes, S. M. Sigoillot, E. Del Nery, F. Selimi, N. Spassky, and A. Genovesio, “Smooth 2D manifold extraction from 3D image stack,” Nat. Commun. 8, 15554 (2017).
[Crossref] [PubMed]

Deng, S.

Diaspro, A.

Ding, J. B.

K. T. Takasaki, J. B. Ding, and B. L. Sabatini, “Live-cell superresolution imaging by pulsed STED two-photon excitation microscopy,” Biophys. J. 104(4), 770–777 (2013).
[Crossref] [PubMed]

Dong, D.

W. Yu, Z. Ji, D. Dong, X. Yang, Y. Xiao, Q. Gong, P. Xi, and K. Shi, “Super-resolution deep imaging with hollow Bessel beam STED microscopy,” Laser Photonics Rev. 10(1), 147–152 (2016).
[Crossref]

Dong, S.

X. Chen, J. Chen, S. Dong, H. Yang, and S. Xie, “Effects of Seidel aberration and light polarization on the resolution of STED imaging,” Optik (Stuttg.) 130, 76–81 (2017).
[Crossref]

Donnert, G.

J. Heine, C. A. Wurm, J. Keller-Findeisen, A. Schönle, B. Harke, M. Reuss, F. R. Winter, and G. Donnert, “Three dimensional live-cell STED microscopy at increased depth using a water immersion objective,” Rev. Sci. Instrum. 89(5), 053701 (2018).
[Crossref] [PubMed]

Dunsby, C.

M. O. Lenz, H. G. Sinclair, A. Savell, J. H. Clegg, A. C. N. Brown, D. M. Davis, C. Dunsby, M. A. A. Neil, and P. M. W. French, “3-D stimulated emission depletion microscopy with programmable aberration correction,” J. Biophotonics 7(1-2), 29–36 (2014).
[Crossref] [PubMed]

E. Auksorius, B. R. Boruah, C. Dunsby, P. M. P. Lanigan, G. Kennedy, M. A. A. Neil, and P. M. W. French, “Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging,” Opt. Lett. 33(2), 113–115 (2008).
[Crossref] [PubMed]

Dyba, M.

M. Dyba and S. W. Hell, “Photostability of a fluorescent marker under pulsed excited-state depletion through stimulated emission,” Appl. Opt. 42(25), 5123–5129 (2003).
[Crossref] [PubMed]

T. A. Klar, S. Jakobs, M. Dyba, A. Egner, and S. W. Hell, “Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission,” Proc. Natl. Acad. Sci. U.S.A. 97(15), 8206–8210 (2000).
[Crossref] [PubMed]

Eggeling, C.

E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, and S. W. Hell, “STED microscopy reveals crystal colour centres with nanometric resolution,” Nat. Photonics 3(3), 144–147 (2009).
[Crossref]

Egner, A.

R. Schmidt, C. A. Wurm, S. Jakobs, J. Engelhardt, A. Egner, and S. W. Hell, “Spherical nanosized focal spot unravels the interior of cells,” Nat. Methods 5(6), 539–544 (2008).
[Crossref] [PubMed]

T. A. Klar, S. Jakobs, M. Dyba, A. Egner, and S. W. Hell, “Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission,” Proc. Natl. Acad. Sci. U.S.A. 97(15), 8206–8210 (2000).
[Crossref] [PubMed]

Eliceiri, K. W.

M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
[Crossref] [PubMed]

Engelhardt, J.

F. Curdt, S. J. Herr, T. Lutz, R. Schmidt, J. Engelhardt, S. J. Sahl, and S. W. Hell, “isoSTED nanoscopy with intrinsic beam alignment,” Opt. Express 23(24), 30891–30903 (2015).
[Crossref] [PubMed]

R. Schmidt, C. A. Wurm, S. Jakobs, J. Engelhardt, A. Egner, and S. W. Hell, “Spherical nanosized focal spot unravels the interior of cells,” Nat. Methods 5(6), 539–544 (2008).
[Crossref] [PubMed]

Erhan, D.

C. Szegedy, W. Liu, Y. Jia, P. Sermanet, S. Reed, D. Anguelov, D. Erhan, V. Vanhoucke, and A. Rabinovich, “Going deeper with convolutions,” in 2015 IEEE Conference on Computer Vision and Pattern Recognition (CVPR) (IEEE, 2015), 39, pp. 1–9.

French, P. M. W.

M. O. Lenz, H. G. Sinclair, A. Savell, J. H. Clegg, A. C. N. Brown, D. M. Davis, C. Dunsby, M. A. A. Neil, and P. M. W. French, “3-D stimulated emission depletion microscopy with programmable aberration correction,” J. Biophotonics 7(1-2), 29–36 (2014).
[Crossref] [PubMed]

E. Auksorius, B. R. Boruah, C. Dunsby, P. M. P. Lanigan, G. Kennedy, M. A. A. Neil, and P. M. W. French, “Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging,” Opt. Lett. 33(2), 113–115 (2008).
[Crossref] [PubMed]

Galiani, S.

Genovesio, A.

A. Shihavuddin, S. Basu, E. Rexhepaj, F. Delestro, N. Menezes, S. M. Sigoillot, E. Del Nery, F. Selimi, N. Spassky, and A. Genovesio, “Smooth 2D manifold extraction from 3D image stack,” Nat. Commun. 8, 15554 (2017).
[Crossref] [PubMed]

S. Basu, E. Rexhepaj, N. Spassky, A. Genovesio, R. Reinhold, and I. Curie, “FastSME : faster and smoother manifold extraction from 3D stack,” in Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition Workshops (2018).
[Crossref]

Gong, Q.

W. Yu, Z. Ji, D. Dong, X. Yang, Y. Xiao, Q. Gong, P. Xi, and K. Shi, “Super-resolution deep imaging with hollow Bessel beam STED microscopy,” Laser Photonics Rev. 10(1), 147–152 (2016).
[Crossref]

Göttfert, F.

F. Göttfert, C. A. Wurm, V. Mueller, S. Berning, V. C. Cordes, A. Honigmann, and S. W. Hell, “Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution,” Biophys. J. 105(1), L01–L03 (2013).
[Crossref] [PubMed]

Gould, T. J.

Hadden, J. P.

D. Wildanger, B. R. Patton, H. Schill, L. Marseglia, J. P. Hadden, S. Knauer, A. Schönle, J. G. Rarity, J. L. O’Brien, S. W. Hell, and J. M. Smith, “Solid immersion facilitates fluorescence microscopy with nanometer resolution and sub-ångström emitter localization,” Adv. Mater. 24(44), OP309–OP313 (2012).
[Crossref] [PubMed]

Han, K. Y.

E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, and S. W. Hell, “STED microscopy reveals crystal colour centres with nanometric resolution,” Nat. Photonics 3(3), 144–147 (2009).
[Crossref]

Hands, K.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

Harke, B.

J. Heine, C. A. Wurm, J. Keller-Findeisen, A. Schönle, B. Harke, M. Reuss, F. R. Winter, and G. Donnert, “Three dimensional live-cell STED microscopy at increased depth using a water immersion objective,” Rev. Sci. Instrum. 89(5), 053701 (2018).
[Crossref] [PubMed]

S. Galiani, B. Harke, G. Vicidomini, G. Lignani, F. Benfenati, A. Diaspro, and P. Bianchini, “Strategies to maximize the performance of a STED microscope,” Opt. Express 20(7), 7362–7374 (2012).
[Crossref] [PubMed]

Hay, R. T.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

Heine, J.

J. Heine, C. A. Wurm, J. Keller-Findeisen, A. Schönle, B. Harke, M. Reuss, F. R. Winter, and G. Donnert, “Three dimensional live-cell STED microscopy at increased depth using a water immersion objective,” Rev. Sci. Instrum. 89(5), 053701 (2018).
[Crossref] [PubMed]

Hell, S. W.

U. Böhm, S. W. Hell, and R. Schmidt, “4Pi-RESOLFT nanoscopy,” Nat. Commun. 7(1), 10504 (2016).
[Crossref] [PubMed]

F. Curdt, S. J. Herr, T. Lutz, R. Schmidt, J. Engelhardt, S. J. Sahl, and S. W. Hell, “isoSTED nanoscopy with intrinsic beam alignment,” Opt. Express 23(24), 30891–30903 (2015).
[Crossref] [PubMed]

F. Göttfert, C. A. Wurm, V. Mueller, S. Berning, V. C. Cordes, A. Honigmann, and S. W. Hell, “Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution,” Biophys. J. 105(1), L01–L03 (2013).
[Crossref] [PubMed]

D. Wildanger, B. R. Patton, H. Schill, L. Marseglia, J. P. Hadden, S. Knauer, A. Schönle, J. G. Rarity, J. L. O’Brien, S. W. Hell, and J. M. Smith, “Solid immersion facilitates fluorescence microscopy with nanometer resolution and sub-ångström emitter localization,” Adv. Mater. 24(44), OP309–OP313 (2012).
[Crossref] [PubMed]

N. T. Urban, K. I. Willig, S. W. Hell, and U. V. Nägerl, “STED nanoscopy of actin dynamics in synapses deep inside living brain slices,” Biophys. J. 101(5), 1277–1284 (2011).
[Crossref] [PubMed]

D. Wildanger, R. Medda, L. Kastrup, and S. W. Hell, “A compact STED microscope providing 3D nanoscale resolution,” J. Microsc. 236(1), 35–43 (2009).
[Crossref] [PubMed]

E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, and S. W. Hell, “STED microscopy reveals crystal colour centres with nanometric resolution,” Nat. Photonics 3(3), 144–147 (2009).
[Crossref]

R. Schmidt, C. A. Wurm, S. Jakobs, J. Engelhardt, A. Egner, and S. W. Hell, “Spherical nanosized focal spot unravels the interior of cells,” Nat. Methods 5(6), 539–544 (2008).
[Crossref] [PubMed]

S. W. Hell, “Far-Field Optical Nanoscopy,” Science 316(5828), 1153–1158 (2007).
[Crossref] [PubMed]

M. Dyba and S. W. Hell, “Photostability of a fluorescent marker under pulsed excited-state depletion through stimulated emission,” Appl. Opt. 42(25), 5123–5129 (2003).
[Crossref] [PubMed]

T. A. Klar, S. Jakobs, M. Dyba, A. Egner, and S. W. Hell, “Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission,” Proc. Natl. Acad. Sci. U.S.A. 97(15), 8206–8210 (2000).
[Crossref] [PubMed]

Herr, S. J.

Hill, E.

M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
[Crossref] [PubMed]

Honigmann, A.

F. Göttfert, C. A. Wurm, V. Mueller, S. Berning, V. C. Cordes, A. Honigmann, and S. W. Hell, “Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution,” Biophys. J. 105(1), L01–L03 (2013).
[Crossref] [PubMed]

Irvine, S. E.

E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, and S. W. Hell, “STED microscopy reveals crystal colour centres with nanometric resolution,” Nat. Photonics 3(3), 144–147 (2009).
[Crossref]

Jakobs, S.

R. Schmidt, C. A. Wurm, S. Jakobs, J. Engelhardt, A. Egner, and S. W. Hell, “Spherical nanosized focal spot unravels the interior of cells,” Nat. Methods 5(6), 539–544 (2008).
[Crossref] [PubMed]

T. A. Klar, S. Jakobs, M. Dyba, A. Egner, and S. W. Hell, “Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission,” Proc. Natl. Acad. Sci. U.S.A. 97(15), 8206–8210 (2000).
[Crossref] [PubMed]

Ji, N.

N. Ji, “Adaptive optical fluorescence microscopy,” Nat. Methods 14(4), 374–380 (2017).
[Crossref] [PubMed]

Ji, Z.

W. Yu, Z. Ji, D. Dong, X. Yang, Y. Xiao, Q. Gong, P. Xi, and K. Shi, “Super-resolution deep imaging with hollow Bessel beam STED microscopy,” Laser Photonics Rev. 10(1), 147–152 (2016).
[Crossref]

Jia, Y.

C. Szegedy, W. Liu, Y. Jia, P. Sermanet, S. Reed, D. Anguelov, D. Erhan, V. Vanhoucke, and A. Rabinovich, “Going deeper with convolutions,” in 2015 IEEE Conference on Computer Vision and Pattern Recognition (CVPR) (IEEE, 2015), 39, pp. 1–9.

Juskaitis, R.

M. J. Booth, M. A. A. Neil, R. Juskaitis, and T. Wilson, “Adaptive aberration correction in a confocal microscope,” Proc. Natl. Acad. Sci. U.S.A. 99(9), 5788–5792 (2002).
[Crossref] [PubMed]

Kastrup, L.

D. Wildanger, R. Medda, L. Kastrup, and S. W. Hell, “A compact STED microscope providing 3D nanoscale resolution,” J. Microsc. 236(1), 35–43 (2009).
[Crossref] [PubMed]

Keller-Findeisen, J.

J. Heine, C. A. Wurm, J. Keller-Findeisen, A. Schönle, B. Harke, M. Reuss, F. R. Winter, and G. Donnert, “Three dimensional live-cell STED microscopy at increased depth using a water immersion objective,” Rev. Sci. Instrum. 89(5), 053701 (2018).
[Crossref] [PubMed]

Kennedy, G.

Kennedy, S. A.

S. A. Kennedy, M. J. Szabo, H. Teslow, J. Z. Porterfield, and E. R. I. Abraham, “Creation of Laguerre-Gaussian laser modes using diffractive optics,” Phys. Rev. A – At. Mol. Opt. Phys. 66, 438011 (2002).

Klar, T. A.

T. A. Klar, S. Jakobs, M. Dyba, A. Egner, and S. W. Hell, “Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission,” Proc. Natl. Acad. Sci. U.S.A. 97(15), 8206–8210 (2000).
[Crossref] [PubMed]

Kleywegt, G. J.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

Knauer, S.

D. Wildanger, B. R. Patton, H. Schill, L. Marseglia, J. P. Hadden, S. Knauer, A. Schönle, J. G. Rarity, J. L. O’Brien, S. W. Hell, and J. M. Smith, “Solid immersion facilitates fluorescence microscopy with nanometer resolution and sub-ångström emitter localization,” Adv. Mater. 24(44), OP309–OP313 (2012).
[Crossref] [PubMed]

Kuang, C.

C. Li, S. Liu, W. Wang, W. Liu, C. Kuang, and X. Liu, “Recent research on stimulated emission depletion microscopy for reducing photobleaching,” J. Microsc. 271(1), 4–16 (2018).
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Lagerstedt, I.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

Lanigan, P. M. P.

Lenz, M. O.

M. O. Lenz, H. G. Sinclair, A. Savell, J. H. Clegg, A. C. N. Brown, D. M. Davis, C. Dunsby, M. A. A. Neil, and P. M. W. French, “3-D stimulated emission depletion microscopy with programmable aberration correction,” J. Biophotonics 7(1-2), 29–36 (2014).
[Crossref] [PubMed]

Leo, S.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

Li, C.

C. Li, S. Liu, W. Wang, W. Liu, C. Kuang, and X. Liu, “Recent research on stimulated emission depletion microscopy for reducing photobleaching,” J. Microsc. 271(1), 4–16 (2018).
[Crossref] [PubMed]

Li, R.

Li, Y.

W. Yan, Y. Yang, Y. Tan, X. Chen, Y. Li, J. Qu, and T. Ye, “Coherent optical adaptive technique improves the spatial resolution of STED microscopy in thick samples,” Photon. Res. 5(3), 176–181 (2017).
[Crossref] [PubMed]

W. Yan, Y. Yang, Y. Li, X. Peng, D. Lin, J. Qu, and T. Ye, “Aberration correction for stimulated emission depletion microscopy with coherent optical adaptive technique,” Adapt. Opt. Wavefront Control Biol. Syst. II9717, 97170K (2016).

Lianas, L.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
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Lignani, G.

Lin, D.

W. Yan, Y. Yang, Y. Li, X. Peng, D. Lin, J. Qu, and T. Ye, “Aberration correction for stimulated emission depletion microscopy with coherent optical adaptive technique,” Adapt. Opt. Wavefront Control Biol. Syst. II9717, 97170K (2016).

Linkert, M.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
[Crossref] [PubMed]

Liu, L.

Liu, S.

C. Li, S. Liu, W. Wang, W. Liu, C. Kuang, and X. Liu, “Recent research on stimulated emission depletion microscopy for reducing photobleaching,” J. Microsc. 271(1), 4–16 (2018).
[Crossref] [PubMed]

Liu, W.

C. Li, S. Liu, W. Wang, W. Liu, C. Kuang, and X. Liu, “Recent research on stimulated emission depletion microscopy for reducing photobleaching,” J. Microsc. 271(1), 4–16 (2018).
[Crossref] [PubMed]

C. Szegedy, W. Liu, Y. Jia, P. Sermanet, S. Reed, D. Anguelov, D. Erhan, V. Vanhoucke, and A. Rabinovich, “Going deeper with convolutions,” in 2015 IEEE Conference on Computer Vision and Pattern Recognition (CVPR) (IEEE, 2015), 39, pp. 1–9.

Liu, X.

C. Li, S. Liu, W. Wang, W. Liu, C. Kuang, and X. Liu, “Recent research on stimulated emission depletion microscopy for reducing photobleaching,” J. Microsc. 271(1), 4–16 (2018).
[Crossref] [PubMed]

Loranger, B.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
[Crossref] [PubMed]

Loynton, S.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
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Lu, R.

Y. Wu, X. Wu, R. Lu, J. Zhang, L. Toro, and E. Stefani, “Resonant Scanning with Large Field of View Reduces Photobleaching and Enhances Fluorescence Yield in STED Microscopy,” Sci. Rep. 5, 14766 (2015).
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Lutz, T.

Macdonald, D.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
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M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
[Crossref] [PubMed]

Marseglia, L.

D. Wildanger, B. R. Patton, H. Schill, L. Marseglia, J. P. Hadden, S. Knauer, A. Schönle, J. G. Rarity, J. L. O’Brien, S. W. Hell, and J. M. Smith, “Solid immersion facilitates fluorescence microscopy with nanometer resolution and sub-ångström emitter localization,” Adv. Mater. 24(44), OP309–OP313 (2012).
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Marsicano, G.

P. Bethge, R. Chéreau, E. Avignone, G. Marsicano, and U. V. Nägerl, “Two-photon excitation STED microscopy in two colors in acute brain slices,” Biophys. J. 104(4), 778–785 (2013).
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Medda, R.

D. Wildanger, R. Medda, L. Kastrup, and S. W. Hell, “A compact STED microscope providing 3D nanoscale resolution,” J. Microsc. 236(1), 35–43 (2009).
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Menezes, N.

A. Shihavuddin, S. Basu, E. Rexhepaj, F. Delestro, N. Menezes, S. M. Sigoillot, E. Del Nery, F. Selimi, N. Spassky, and A. Genovesio, “Smooth 2D manifold extraction from 3D image stack,” Nat. Commun. 8, 15554 (2017).
[Crossref] [PubMed]

Moore, J.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
[Crossref] [PubMed]

Moore, W.

M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
[Crossref] [PubMed]

Moore, W. J.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
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Mueller, V.

F. Göttfert, C. A. Wurm, V. Mueller, S. Berning, V. C. Cordes, A. Honigmann, and S. W. Hell, “Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution,” Biophys. J. 105(1), L01–L03 (2013).
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Munro, P.

Nägerl, U. V.

P. Bethge, R. Chéreau, E. Avignone, G. Marsicano, and U. V. Nägerl, “Two-photon excitation STED microscopy in two colors in acute brain slices,” Biophys. J. 104(4), 778–785 (2013).
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N. T. Urban, K. I. Willig, S. W. Hell, and U. V. Nägerl, “STED nanoscopy of actin dynamics in synapses deep inside living brain slices,” Biophys. J. 101(5), 1277–1284 (2011).
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Neil, M. A. A.

M. O. Lenz, H. G. Sinclair, A. Savell, J. H. Clegg, A. C. N. Brown, D. M. Davis, C. Dunsby, M. A. A. Neil, and P. M. W. French, “3-D stimulated emission depletion microscopy with programmable aberration correction,” J. Biophotonics 7(1-2), 29–36 (2014).
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E. Auksorius, B. R. Boruah, C. Dunsby, P. M. P. Lanigan, G. Kennedy, M. A. A. Neil, and P. M. W. French, “Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging,” Opt. Lett. 33(2), 113–115 (2008).
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M. J. Booth, M. A. A. Neil, R. Juskaitis, and T. Wilson, “Adaptive aberration correction in a confocal microscope,” Proc. Natl. Acad. Sci. U.S.A. 99(9), 5788–5792 (2002).
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Neves, C.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
[Crossref] [PubMed]

O’Brien, J. L.

D. Wildanger, B. R. Patton, H. Schill, L. Marseglia, J. P. Hadden, S. Knauer, A. Schönle, J. G. Rarity, J. L. O’Brien, S. W. Hell, and J. M. Smith, “Solid immersion facilitates fluorescence microscopy with nanometer resolution and sub-ångström emitter localization,” Adv. Mater. 24(44), OP309–OP313 (2012).
[Crossref] [PubMed]

Owald, D.

Padgett, M. J.

Patterson, A.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
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M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
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Patton, B. R.

B. R. Patton, D. Burke, D. Owald, T. J. Gould, J. Bewersdorf, and M. J. Booth, “Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics,” Opt. Express 24(8), 8862–8876 (2016).
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B. R. Patton, D. Burke, R. Vrees, and M. J. Booth, “Is phase-mask alignment aberrating your STED microscope?” Methods Appl. Fluoresc. 3(2), 024002 (2015).
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D. Wildanger, B. R. Patton, H. Schill, L. Marseglia, J. P. Hadden, S. Knauer, A. Schönle, J. G. Rarity, J. L. O’Brien, S. W. Hell, and J. M. Smith, “Solid immersion facilitates fluorescence microscopy with nanometer resolution and sub-ångström emitter localization,” Adv. Mater. 24(44), OP309–OP313 (2012).
[Crossref] [PubMed]

Patwardhan, A.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
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Peng, X.

W. Yan, Y. Yang, Y. Li, X. Peng, D. Lin, J. Qu, and T. Ye, “Aberration correction for stimulated emission depletion microscopy with coherent optical adaptive technique,” Adapt. Opt. Wavefront Control Biol. Syst. II9717, 97170K (2016).

Porter, M.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
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Porterfield, J. Z.

S. A. Kennedy, M. J. Szabo, H. Teslow, J. Z. Porterfield, and E. R. I. Abraham, “Creation of Laguerre-Gaussian laser modes using diffractive optics,” Phys. Rev. A – At. Mol. Opt. Phys. 66, 438011 (2002).

Qu, J.

W. Yan, Y. Yang, Y. Tan, X. Chen, Y. Li, J. Qu, and T. Ye, “Coherent optical adaptive technique improves the spatial resolution of STED microscopy in thick samples,” Photon. Res. 5(3), 176–181 (2017).
[Crossref] [PubMed]

W. Yan, Y. Yang, Y. Li, X. Peng, D. Lin, J. Qu, and T. Ye, “Aberration correction for stimulated emission depletion microscopy with coherent optical adaptive technique,” Adapt. Opt. Wavefront Control Biol. Syst. II9717, 97170K (2016).

Rabinovich, A.

C. Szegedy, W. Liu, Y. Jia, P. Sermanet, S. Reed, D. Anguelov, D. Erhan, V. Vanhoucke, and A. Rabinovich, “Going deeper with convolutions,” in 2015 IEEE Conference on Computer Vision and Pattern Recognition (CVPR) (IEEE, 2015), 39, pp. 1–9.

Rarity, J. G.

D. Wildanger, B. R. Patton, H. Schill, L. Marseglia, J. P. Hadden, S. Knauer, A. Schönle, J. G. Rarity, J. L. O’Brien, S. W. Hell, and J. M. Smith, “Solid immersion facilitates fluorescence microscopy with nanometer resolution and sub-ångström emitter localization,” Adv. Mater. 24(44), OP309–OP313 (2012).
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Reed, S.

C. Szegedy, W. Liu, Y. Jia, P. Sermanet, S. Reed, D. Anguelov, D. Erhan, V. Vanhoucke, and A. Rabinovich, “Going deeper with convolutions,” in 2015 IEEE Conference on Computer Vision and Pattern Recognition (CVPR) (IEEE, 2015), 39, pp. 1–9.

Reinhold, R.

S. Basu, E. Rexhepaj, N. Spassky, A. Genovesio, R. Reinhold, and I. Curie, “FastSME : faster and smoother manifold extraction from 3D stack,” in Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition Workshops (2018).
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Reuss, M.

J. Heine, C. A. Wurm, J. Keller-Findeisen, A. Schönle, B. Harke, M. Reuss, F. R. Winter, and G. Donnert, “Three dimensional live-cell STED microscopy at increased depth using a water immersion objective,” Rev. Sci. Instrum. 89(5), 053701 (2018).
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Rexhepaj, E.

A. Shihavuddin, S. Basu, E. Rexhepaj, F. Delestro, N. Menezes, S. M. Sigoillot, E. Del Nery, F. Selimi, N. Spassky, and A. Genovesio, “Smooth 2D manifold extraction from 3D image stack,” Nat. Commun. 8, 15554 (2017).
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S. Basu, E. Rexhepaj, N. Spassky, A. Genovesio, R. Reinhold, and I. Curie, “FastSME : faster and smoother manifold extraction from 3D stack,” in Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition Workshops (2018).
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Rittweger, E.

E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, and S. W. Hell, “STED microscopy reveals crystal colour centres with nanometric resolution,” Nat. Photonics 3(3), 144–147 (2009).
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Rossner, M.

M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
[Crossref] [PubMed]

Rueden, C. T.

M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
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Sabatini, B. L.

K. T. Takasaki, J. B. Ding, and B. L. Sabatini, “Live-cell superresolution imaging by pulsed STED two-photon excitation microscopy,” Biophys. J. 104(4), 770–777 (2013).
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Sahl, S. J.

Savell, A.

M. O. Lenz, H. G. Sinclair, A. Savell, J. H. Clegg, A. C. N. Brown, D. M. Davis, C. Dunsby, M. A. A. Neil, and P. M. W. French, “3-D stimulated emission depletion microscopy with programmable aberration correction,” J. Biophotonics 7(1-2), 29–36 (2014).
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Schill, H.

D. Wildanger, B. R. Patton, H. Schill, L. Marseglia, J. P. Hadden, S. Knauer, A. Schönle, J. G. Rarity, J. L. O’Brien, S. W. Hell, and J. M. Smith, “Solid immersion facilitates fluorescence microscopy with nanometer resolution and sub-ångström emitter localization,” Adv. Mater. 24(44), OP309–OP313 (2012).
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Schmidt, R.

U. Böhm, S. W. Hell, and R. Schmidt, “4Pi-RESOLFT nanoscopy,” Nat. Commun. 7(1), 10504 (2016).
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F. Curdt, S. J. Herr, T. Lutz, R. Schmidt, J. Engelhardt, S. J. Sahl, and S. W. Hell, “isoSTED nanoscopy with intrinsic beam alignment,” Opt. Express 23(24), 30891–30903 (2015).
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Schönle, A.

J. Heine, C. A. Wurm, J. Keller-Findeisen, A. Schönle, B. Harke, M. Reuss, F. R. Winter, and G. Donnert, “Three dimensional live-cell STED microscopy at increased depth using a water immersion objective,” Rev. Sci. Instrum. 89(5), 053701 (2018).
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D. Wildanger, B. R. Patton, H. Schill, L. Marseglia, J. P. Hadden, S. Knauer, A. Schönle, J. G. Rarity, J. L. O’Brien, S. W. Hell, and J. M. Smith, “Solid immersion facilitates fluorescence microscopy with nanometer resolution and sub-ångström emitter localization,” Adv. Mater. 24(44), OP309–OP313 (2012).
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Selimi, F.

A. Shihavuddin, S. Basu, E. Rexhepaj, F. Delestro, N. Menezes, S. M. Sigoillot, E. Del Nery, F. Selimi, N. Spassky, and A. Genovesio, “Smooth 2D manifold extraction from 3D image stack,” Nat. Commun. 8, 15554 (2017).
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Sermanet, P.

C. Szegedy, W. Liu, Y. Jia, P. Sermanet, S. Reed, D. Anguelov, D. Erhan, V. Vanhoucke, and A. Rabinovich, “Going deeper with convolutions,” in 2015 IEEE Conference on Computer Vision and Pattern Recognition (CVPR) (IEEE, 2015), 39, pp. 1–9.

Shi, K.

W. Yu, Z. Ji, D. Dong, X. Yang, Y. Xiao, Q. Gong, P. Xi, and K. Shi, “Super-resolution deep imaging with hollow Bessel beam STED microscopy,” Laser Photonics Rev. 10(1), 147–152 (2016).
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A. Shihavuddin, S. Basu, E. Rexhepaj, F. Delestro, N. Menezes, S. M. Sigoillot, E. Del Nery, F. Selimi, N. Spassky, and A. Genovesio, “Smooth 2D manifold extraction from 3D image stack,” Nat. Commun. 8, 15554 (2017).
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Sigoillot, S. M.

A. Shihavuddin, S. Basu, E. Rexhepaj, F. Delestro, N. Menezes, S. M. Sigoillot, E. Del Nery, F. Selimi, N. Spassky, and A. Genovesio, “Smooth 2D manifold extraction from 3D image stack,” Nat. Commun. 8, 15554 (2017).
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Sinclair, H. G.

M. O. Lenz, H. G. Sinclair, A. Savell, J. H. Clegg, A. C. N. Brown, D. M. Davis, C. Dunsby, M. A. A. Neil, and P. M. W. French, “3-D stimulated emission depletion microscopy with programmable aberration correction,” J. Biophotonics 7(1-2), 29–36 (2014).
[Crossref] [PubMed]

Smith, J. M.

D. Wildanger, B. R. Patton, H. Schill, L. Marseglia, J. P. Hadden, S. Knauer, A. Schönle, J. G. Rarity, J. L. O’Brien, S. W. Hell, and J. M. Smith, “Solid immersion facilitates fluorescence microscopy with nanometer resolution and sub-ångström emitter localization,” Adv. Mater. 24(44), OP309–OP313 (2012).
[Crossref] [PubMed]

Spassky, N.

A. Shihavuddin, S. Basu, E. Rexhepaj, F. Delestro, N. Menezes, S. M. Sigoillot, E. Del Nery, F. Selimi, N. Spassky, and A. Genovesio, “Smooth 2D manifold extraction from 3D image stack,” Nat. Commun. 8, 15554 (2017).
[Crossref] [PubMed]

S. Basu, E. Rexhepaj, N. Spassky, A. Genovesio, R. Reinhold, and I. Curie, “FastSME : faster and smoother manifold extraction from 3D stack,” in Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition Workshops (2018).
[Crossref]

Stefani, E.

X. Wu, L. Toro, E. Stefani, and Y. Wu, “Ultrafast photon counting applied to resonant scanning STED microscopy,” J. Microsc. 257(1), 31–38 (2015).
[Crossref] [PubMed]

Y. Wu, X. Wu, R. Lu, J. Zhang, L. Toro, and E. Stefani, “Resonant Scanning with Large Field of View Reduces Photobleaching and Enhances Fluorescence Yield in STED Microscopy,” Sci. Rep. 5, 14766 (2015).
[Crossref] [PubMed]

Sticco, C.

M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
[Crossref] [PubMed]

Swedlow, J. R.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
[Crossref] [PubMed]

Szabo, M. J.

S. A. Kennedy, M. J. Szabo, H. Teslow, J. Z. Porterfield, and E. R. I. Abraham, “Creation of Laguerre-Gaussian laser modes using diffractive optics,” Phys. Rev. A – At. Mol. Opt. Phys. 66, 438011 (2002).

Szegedy, C.

C. Szegedy, W. Liu, Y. Jia, P. Sermanet, S. Reed, D. Anguelov, D. Erhan, V. Vanhoucke, and A. Rabinovich, “Going deeper with convolutions,” in 2015 IEEE Conference on Computer Vision and Pattern Recognition (CVPR) (IEEE, 2015), 39, pp. 1–9.

Takasaki, K. T.

K. T. Takasaki, J. B. Ding, and B. L. Sabatini, “Live-cell superresolution imaging by pulsed STED two-photon excitation microscopy,” Biophys. J. 104(4), 770–777 (2013).
[Crossref] [PubMed]

Tan, Y.

Tarkowska, A.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
[Crossref] [PubMed]

Teslow, H.

S. A. Kennedy, M. J. Szabo, H. Teslow, J. Z. Porterfield, and E. R. I. Abraham, “Creation of Laguerre-Gaussian laser modes using diffractive optics,” Phys. Rev. A – At. Mol. Opt. Phys. 66, 438011 (2002).

Toro, L.

Y. Wu, X. Wu, R. Lu, J. Zhang, L. Toro, and E. Stefani, “Resonant Scanning with Large Field of View Reduces Photobleaching and Enhances Fluorescence Yield in STED Microscopy,” Sci. Rep. 5, 14766 (2015).
[Crossref] [PubMed]

X. Wu, L. Toro, E. Stefani, and Y. Wu, “Ultrafast photon counting applied to resonant scanning STED microscopy,” J. Microsc. 257(1), 31–38 (2015).
[Crossref] [PubMed]

Török, P.

Urban, N. T.

N. T. Urban, K. I. Willig, S. W. Hell, and U. V. Nägerl, “STED nanoscopy of actin dynamics in synapses deep inside living brain slices,” Biophys. J. 101(5), 1277–1284 (2011).
[Crossref] [PubMed]

Vanhoucke, V.

C. Szegedy, W. Liu, Y. Jia, P. Sermanet, S. Reed, D. Anguelov, D. Erhan, V. Vanhoucke, and A. Rabinovich, “Going deeper with convolutions,” in 2015 IEEE Conference on Computer Vision and Pattern Recognition (CVPR) (IEEE, 2015), 39, pp. 1–9.

Vellekoop, I. M.

Vicidomini, G.

Vrees, R.

B. R. Patton, D. Burke, R. Vrees, and M. J. Booth, “Is phase-mask alignment aberrating your STED microscope?” Methods Appl. Fluoresc. 3(2), 024002 (2015).
[Crossref] [PubMed]

Wang, W.

C. Li, S. Liu, W. Wang, W. Liu, C. Kuang, and X. Liu, “Recent research on stimulated emission depletion microscopy for reducing photobleaching,” J. Microsc. 271(1), 4–16 (2018).
[Crossref] [PubMed]

Widengren, J.

H. Blom and J. Widengren, “Stimulated Emission Depletion Microscopy,” Chem. Rev. 117(11), 7377–7427 (2017).
[Crossref] [PubMed]

Wildanger, D.

D. Wildanger, B. R. Patton, H. Schill, L. Marseglia, J. P. Hadden, S. Knauer, A. Schönle, J. G. Rarity, J. L. O’Brien, S. W. Hell, and J. M. Smith, “Solid immersion facilitates fluorescence microscopy with nanometer resolution and sub-ångström emitter localization,” Adv. Mater. 24(44), OP309–OP313 (2012).
[Crossref] [PubMed]

D. Wildanger, R. Medda, L. Kastrup, and S. W. Hell, “A compact STED microscope providing 3D nanoscale resolution,” J. Microsc. 236(1), 35–43 (2009).
[Crossref] [PubMed]

Willig, K. I.

N. T. Urban, K. I. Willig, S. W. Hell, and U. V. Nägerl, “STED nanoscopy of actin dynamics in synapses deep inside living brain slices,” Biophys. J. 101(5), 1277–1284 (2011).
[Crossref] [PubMed]

Wilson, T.

D. Debarre, M. J. Booth, and T. Wilson, “Image based adaptive optics through optimisation of low spatial frequencies,” Opt. Express 15(13), 8176–8190 (2007).
[Crossref] [PubMed]

M. J. Booth, M. A. A. Neil, R. Juskaitis, and T. Wilson, “Adaptive aberration correction in a confocal microscope,” Proc. Natl. Acad. Sci. U.S.A. 99(9), 5788–5792 (2002).
[Crossref] [PubMed]

Winter, F. R.

J. Heine, C. A. Wurm, J. Keller-Findeisen, A. Schönle, B. Harke, M. Reuss, F. R. Winter, and G. Donnert, “Three dimensional live-cell STED microscopy at increased depth using a water immersion objective,” Rev. Sci. Instrum. 89(5), 053701 (2018).
[Crossref] [PubMed]

Wu, X.

Y. Wu, X. Wu, R. Lu, J. Zhang, L. Toro, and E. Stefani, “Resonant Scanning with Large Field of View Reduces Photobleaching and Enhances Fluorescence Yield in STED Microscopy,” Sci. Rep. 5, 14766 (2015).
[Crossref] [PubMed]

X. Wu, L. Toro, E. Stefani, and Y. Wu, “Ultrafast photon counting applied to resonant scanning STED microscopy,” J. Microsc. 257(1), 31–38 (2015).
[Crossref] [PubMed]

Wu, Y.

X. Wu, L. Toro, E. Stefani, and Y. Wu, “Ultrafast photon counting applied to resonant scanning STED microscopy,” J. Microsc. 257(1), 31–38 (2015).
[Crossref] [PubMed]

Y. Wu, X. Wu, R. Lu, J. Zhang, L. Toro, and E. Stefani, “Resonant Scanning with Large Field of View Reduces Photobleaching and Enhances Fluorescence Yield in STED Microscopy,” Sci. Rep. 5, 14766 (2015).
[Crossref] [PubMed]

Wurm, C. A.

J. Heine, C. A. Wurm, J. Keller-Findeisen, A. Schönle, B. Harke, M. Reuss, F. R. Winter, and G. Donnert, “Three dimensional live-cell STED microscopy at increased depth using a water immersion objective,” Rev. Sci. Instrum. 89(5), 053701 (2018).
[Crossref] [PubMed]

F. Göttfert, C. A. Wurm, V. Mueller, S. Berning, V. C. Cordes, A. Honigmann, and S. W. Hell, “Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution,” Biophys. J. 105(1), L01–L03 (2013).
[Crossref] [PubMed]

R. Schmidt, C. A. Wurm, S. Jakobs, J. Engelhardt, A. Egner, and S. W. Hell, “Spherical nanosized focal spot unravels the interior of cells,” Nat. Methods 5(6), 539–544 (2008).
[Crossref] [PubMed]

Xi, P.

W. Yu, Z. Ji, D. Dong, X. Yang, Y. Xiao, Q. Gong, P. Xi, and K. Shi, “Super-resolution deep imaging with hollow Bessel beam STED microscopy,” Laser Photonics Rev. 10(1), 147–152 (2016).
[Crossref]

Xiao, Y.

W. Yu, Z. Ji, D. Dong, X. Yang, Y. Xiao, Q. Gong, P. Xi, and K. Shi, “Super-resolution deep imaging with hollow Bessel beam STED microscopy,” Laser Photonics Rev. 10(1), 147–152 (2016).
[Crossref]

Xie, S.

X. Chen, J. Chen, S. Dong, H. Yang, and S. Xie, “Effects of Seidel aberration and light polarization on the resolution of STED imaging,” Optik (Stuttg.) 130, 76–81 (2017).
[Crossref]

Xu, Z.

Yan, W.

W. Yan, Y. Yang, Y. Tan, X. Chen, Y. Li, J. Qu, and T. Ye, “Coherent optical adaptive technique improves the spatial resolution of STED microscopy in thick samples,” Photon. Res. 5(3), 176–181 (2017).
[Crossref] [PubMed]

W. Yan, Y. Yang, Y. Li, X. Peng, D. Lin, J. Qu, and T. Ye, “Aberration correction for stimulated emission depletion microscopy with coherent optical adaptive technique,” Adapt. Opt. Wavefront Control Biol. Syst. II9717, 97170K (2016).

Yang, H.

X. Chen, J. Chen, S. Dong, H. Yang, and S. Xie, “Effects of Seidel aberration and light polarization on the resolution of STED imaging,” Optik (Stuttg.) 130, 76–81 (2017).
[Crossref]

Yang, X.

W. Yu, Z. Ji, D. Dong, X. Yang, Y. Xiao, Q. Gong, P. Xi, and K. Shi, “Super-resolution deep imaging with hollow Bessel beam STED microscopy,” Laser Photonics Rev. 10(1), 147–152 (2016).
[Crossref]

Yang, Y.

W. Yan, Y. Yang, Y. Tan, X. Chen, Y. Li, J. Qu, and T. Ye, “Coherent optical adaptive technique improves the spatial resolution of STED microscopy in thick samples,” Photon. Res. 5(3), 176–181 (2017).
[Crossref] [PubMed]

W. Yan, Y. Yang, Y. Li, X. Peng, D. Lin, J. Qu, and T. Ye, “Aberration correction for stimulated emission depletion microscopy with coherent optical adaptive technique,” Adapt. Opt. Wavefront Control Biol. Syst. II9717, 97170K (2016).

Ye, T.

W. Yan, Y. Yang, Y. Tan, X. Chen, Y. Li, J. Qu, and T. Ye, “Coherent optical adaptive technique improves the spatial resolution of STED microscopy in thick samples,” Photon. Res. 5(3), 176–181 (2017).
[Crossref] [PubMed]

W. Yan, Y. Yang, Y. Li, X. Peng, D. Lin, J. Qu, and T. Ye, “Aberration correction for stimulated emission depletion microscopy with coherent optical adaptive technique,” Adapt. Opt. Wavefront Control Biol. Syst. II9717, 97170K (2016).

Yu, W.

W. Yu, Z. Ji, D. Dong, X. Yang, Y. Xiao, Q. Gong, P. Xi, and K. Shi, “Super-resolution deep imaging with hollow Bessel beam STED microscopy,” Laser Photonics Rev. 10(1), 147–152 (2016).
[Crossref]

Zanetti, G.

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

Zhang, J.

Y. Wu, X. Wu, R. Lu, J. Zhang, L. Toro, and E. Stefani, “Resonant Scanning with Large Field of View Reduces Photobleaching and Enhances Fluorescence Yield in STED Microscopy,” Sci. Rep. 5, 14766 (2015).
[Crossref] [PubMed]

Adv. Mater. (1)

D. Wildanger, B. R. Patton, H. Schill, L. Marseglia, J. P. Hadden, S. Knauer, A. Schönle, J. G. Rarity, J. L. O’Brien, S. W. Hell, and J. M. Smith, “Solid immersion facilitates fluorescence microscopy with nanometer resolution and sub-ångström emitter localization,” Adv. Mater. 24(44), OP309–OP313 (2012).
[Crossref] [PubMed]

Appl. Opt. (1)

Biophys. J. (4)

F. Göttfert, C. A. Wurm, V. Mueller, S. Berning, V. C. Cordes, A. Honigmann, and S. W. Hell, “Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution,” Biophys. J. 105(1), L01–L03 (2013).
[Crossref] [PubMed]

N. T. Urban, K. I. Willig, S. W. Hell, and U. V. Nägerl, “STED nanoscopy of actin dynamics in synapses deep inside living brain slices,” Biophys. J. 101(5), 1277–1284 (2011).
[Crossref] [PubMed]

K. T. Takasaki, J. B. Ding, and B. L. Sabatini, “Live-cell superresolution imaging by pulsed STED two-photon excitation microscopy,” Biophys. J. 104(4), 770–777 (2013).
[Crossref] [PubMed]

P. Bethge, R. Chéreau, E. Avignone, G. Marsicano, and U. V. Nägerl, “Two-photon excitation STED microscopy in two colors in acute brain slices,” Biophys. J. 104(4), 778–785 (2013).
[Crossref] [PubMed]

Chem. Rev. (1)

H. Blom and J. Widengren, “Stimulated Emission Depletion Microscopy,” Chem. Rev. 117(11), 7377–7427 (2017).
[Crossref] [PubMed]

J. Biophotonics (1)

M. O. Lenz, H. G. Sinclair, A. Savell, J. H. Clegg, A. C. N. Brown, D. M. Davis, C. Dunsby, M. A. A. Neil, and P. M. W. French, “3-D stimulated emission depletion microscopy with programmable aberration correction,” J. Biophotonics 7(1-2), 29–36 (2014).
[Crossref] [PubMed]

J. Cell Biol. (1)

M. Linkert, C. T. Rueden, C. Allan, J. M. Burel, W. Moore, A. Patterson, B. Loranger, J. Moore, C. Neves, D. Macdonald, A. Tarkowska, C. Sticco, E. Hill, M. Rossner, K. W. Eliceiri, and J. R. Swedlow, “Metadata matters: Access to image data in the real world,” J. Cell Biol. 189(5), 777–782 (2010).
[Crossref] [PubMed]

J. Microsc. (3)

X. Wu, L. Toro, E. Stefani, and Y. Wu, “Ultrafast photon counting applied to resonant scanning STED microscopy,” J. Microsc. 257(1), 31–38 (2015).
[Crossref] [PubMed]

C. Li, S. Liu, W. Wang, W. Liu, C. Kuang, and X. Liu, “Recent research on stimulated emission depletion microscopy for reducing photobleaching,” J. Microsc. 271(1), 4–16 (2018).
[Crossref] [PubMed]

D. Wildanger, R. Medda, L. Kastrup, and S. W. Hell, “A compact STED microscope providing 3D nanoscale resolution,” J. Microsc. 236(1), 35–43 (2009).
[Crossref] [PubMed]

Laser Photonics Rev. (1)

W. Yu, Z. Ji, D. Dong, X. Yang, Y. Xiao, Q. Gong, P. Xi, and K. Shi, “Super-resolution deep imaging with hollow Bessel beam STED microscopy,” Laser Photonics Rev. 10(1), 147–152 (2016).
[Crossref]

Light Sci. Appl. (1)

M. J. Booth, “Adaptive optical microscopy: The ongoing quest for a perfect image,” Light Sci. Appl. 3(4), e165 (2014).
[Crossref]

Methods Appl. Fluoresc. (1)

B. R. Patton, D. Burke, R. Vrees, and M. J. Booth, “Is phase-mask alignment aberrating your STED microscope?” Methods Appl. Fluoresc. 3(2), 024002 (2015).
[Crossref] [PubMed]

Nat. Commun. (2)

U. Böhm, S. W. Hell, and R. Schmidt, “4Pi-RESOLFT nanoscopy,” Nat. Commun. 7(1), 10504 (2016).
[Crossref] [PubMed]

A. Shihavuddin, S. Basu, E. Rexhepaj, F. Delestro, N. Menezes, S. M. Sigoillot, E. Del Nery, F. Selimi, N. Spassky, and A. Genovesio, “Smooth 2D manifold extraction from 3D image stack,” Nat. Commun. 8, 15554 (2017).
[Crossref] [PubMed]

Nat. Methods (3)

C. Allan, J.-M. Burel, J. Moore, C. Blackburn, M. Linkert, S. Loynton, D. Macdonald, W. J. Moore, C. Neves, A. Patterson, M. Porter, A. Tarkowska, B. Loranger, J. Avondo, I. Lagerstedt, L. Lianas, S. Leo, K. Hands, R. T. Hay, A. Patwardhan, C. Best, G. J. Kleywegt, G. Zanetti, and J. R. Swedlow, “OMERO: flexible, model-driven data management for experimental biology,” Nat. Methods 9(3), 245–253 (2012).
[Crossref] [PubMed]

N. Ji, “Adaptive optical fluorescence microscopy,” Nat. Methods 14(4), 374–380 (2017).
[Crossref] [PubMed]

R. Schmidt, C. A. Wurm, S. Jakobs, J. Engelhardt, A. Egner, and S. W. Hell, “Spherical nanosized focal spot unravels the interior of cells,” Nat. Methods 5(6), 539–544 (2008).
[Crossref] [PubMed]

Nat. Photonics (1)

E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, and S. W. Hell, “STED microscopy reveals crystal colour centres with nanometric resolution,” Nat. Photonics 3(3), 144–147 (2009).
[Crossref]

Opt. Commun. (1)

J. Antonello, D. Burke, and M. J. Booth, “Aberrations in stimulated emission depletion (STED) microscopy,” Opt. Commun. 404, 203–209 (2017).
[Crossref] [PubMed]

Opt. Express (9)

S. Deng, L. Liu, Y. Cheng, R. Li, and Z. Xu, “Effects of primary aberrations on the fluorescence depletion patterns of STED microscopy,” Opt. Express 18(2), 1657–1666 (2010).
[Crossref] [PubMed]

S. Deng, L. Liu, Y. Cheng, R. Li, and Z. Xu, “Investigation of the influence of the aberration induced by a plane interface on STED microscopy,” Opt. Express 17(3), 1714–1725 (2009).
[Crossref] [PubMed]

B. R. Patton, D. Burke, D. Owald, T. J. Gould, J. Bewersdorf, and M. J. Booth, “Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics,” Opt. Express 24(8), 8862–8876 (2016).
[Crossref] [PubMed]

S. Galiani, B. Harke, G. Vicidomini, G. Lignani, F. Benfenati, A. Diaspro, and P. Bianchini, “Strategies to maximize the performance of a STED microscope,” Opt. Express 20(7), 7362–7374 (2012).
[Crossref] [PubMed]

I. M. Vellekoop, “Feedback-based wavefront shaping,” Opt. Express 23(9), 12189–12206 (2015).
[Crossref] [PubMed]

F. Curdt, S. J. Herr, T. Lutz, R. Schmidt, J. Engelhardt, S. J. Sahl, and S. W. Hell, “isoSTED nanoscopy with intrinsic beam alignment,” Opt. Express 23(24), 30891–30903 (2015).
[Crossref] [PubMed]

P. Török and P. Munro, “The use of Gauss-Laguerre vector beams in STED microscopy,” Opt. Express 12(15), 3605–3617 (2004).
[Crossref] [PubMed]

T. J. Gould, D. Burke, J. Bewersdorf, and M. J. Booth, “Adaptive optics enables 3D STED microscopy in aberrating specimens,” Opt. Express 20(19), 20998–21009 (2012).
[Crossref] [PubMed]

D. Debarre, M. J. Booth, and T. Wilson, “Image based adaptive optics through optimisation of low spatial frequencies,” Opt. Express 15(13), 8176–8190 (2007).
[Crossref] [PubMed]

Opt. Lett. (2)

Optik (Stuttg.) (1)

X. Chen, J. Chen, S. Dong, H. Yang, and S. Xie, “Effects of Seidel aberration and light polarization on the resolution of STED imaging,” Optik (Stuttg.) 130, 76–81 (2017).
[Crossref]

Photon. Res. (1)

Phys. Rev. A – At. Mol. Opt. Phys. (1)

S. A. Kennedy, M. J. Szabo, H. Teslow, J. Z. Porterfield, and E. R. I. Abraham, “Creation of Laguerre-Gaussian laser modes using diffractive optics,” Phys. Rev. A – At. Mol. Opt. Phys. 66, 438011 (2002).

Proc. Natl. Acad. Sci. U.S.A. (2)

T. A. Klar, S. Jakobs, M. Dyba, A. Egner, and S. W. Hell, “Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission,” Proc. Natl. Acad. Sci. U.S.A. 97(15), 8206–8210 (2000).
[Crossref] [PubMed]

M. J. Booth, M. A. A. Neil, R. Juskaitis, and T. Wilson, “Adaptive aberration correction in a confocal microscope,” Proc. Natl. Acad. Sci. U.S.A. 99(9), 5788–5792 (2002).
[Crossref] [PubMed]

Rev. Sci. Instrum. (1)

J. Heine, C. A. Wurm, J. Keller-Findeisen, A. Schönle, B. Harke, M. Reuss, F. R. Winter, and G. Donnert, “Three dimensional live-cell STED microscopy at increased depth using a water immersion objective,” Rev. Sci. Instrum. 89(5), 053701 (2018).
[Crossref] [PubMed]

Sci. Rep. (1)

Y. Wu, X. Wu, R. Lu, J. Zhang, L. Toro, and E. Stefani, “Resonant Scanning with Large Field of View Reduces Photobleaching and Enhances Fluorescence Yield in STED Microscopy,” Sci. Rep. 5, 14766 (2015).
[Crossref] [PubMed]

Science (1)

S. W. Hell, “Far-Field Optical Nanoscopy,” Science 316(5828), 1153–1158 (2007).
[Crossref] [PubMed]

Other (6)

W. Yan, Y. Yang, Y. Li, X. Peng, D. Lin, J. Qu, and T. Ye, “Aberration correction for stimulated emission depletion microscopy with coherent optical adaptive technique,” Adapt. Opt. Wavefront Control Biol. Syst. II9717, 97170K (2016).

M. J. Booth, “Adaptive Optics in Microscopy,” Opt. Digit. Image Process. Fundam. Appl. 295–322 (2011).
[Crossref]

H. Gross, H. Zügge, M. Peschka, and F. Blechinger, Handbook of Optical Systems Volume 3: Aberration Theory and Correction of Optical Systems (Wiley-VCH Verlag GmbH & Co. KGaA, 2006), Vol. 2.

C. Szegedy, W. Liu, Y. Jia, P. Sermanet, S. Reed, D. Anguelov, D. Erhan, V. Vanhoucke, and A. Rabinovich, “Going deeper with convolutions,” in 2015 IEEE Conference on Computer Vision and Pattern Recognition (CVPR) (IEEE, 2015), 39, pp. 1–9.

S. Basu, E. Rexhepaj, N. Spassky, A. Genovesio, R. Reinhold, and I. Curie, “FastSME : faster and smoother manifold extraction from 3D stack,” in Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition Workshops (2018).
[Crossref]

R. C. Gonzalez and R. E. Woods, Digital Image Processing (3rd Edition) (Prentice-Hall, Inc., 2006).

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Figures (6)

Fig. 1
Fig. 1 The optical setup of the custom-built AO 3D STED microscope. M1-M3 are beam steering mirrors, MO - microscope objective, f1-f11 and fi are lenses, FC - fibre coupling lens, RM - resonant mirror, GM - pair of galvanometric mirrors, PMF - polarisation maintaining single mode fibre, PH - pinhole, DM1-DM2 are dichroic mirrors as indicated in the text, GLP - glan laser polariser λ/2 - half-wave plate, λ /4 - quarter-wave plate, EF - emission filter, SPAD - single photon avalanche diode, SLM - spatial light modulator. Detailed description of the setup and its elements can be found in text.
Fig. 2
Fig. 2 Comparison of the mitotic spindle acquired with (a) confocal and (b) 2D STED. Images are displayed with the cubehelix colormap. The insets in (a) and (b) show the zoomed area marked with the dashed rectangle. The plot in (c) shows the intensity cross-section at the position shown by the yellow line in the zoomed images in (a) and (b). The blue plot shows the cross-section through the confocal image, red is the cross-section through 2D STED image and the green plots represents the Lorentzian fit to the 2D STED cross-section. The slices shown are at a depth of 5µm from the bottom of the mitotic cell. The scale bar is 1µm.
Fig. 3
Fig. 3 Slices of the mitotic spindle acquired with the 2D STED mode at different depths measured from the bottom of the cell: (a) 1µm from the bottom and (b) 10µm from the bottom. Insets in the images show the zoomed area marked with the yellow dashed rectangle. Plots in (c) and (d) show the intensity cross-sections for the microtubules, at the position marked with the yellow line inside the insets. The scale bar on both images are 1µm.
Fig. 4
Fig. 4 Comparison of the xz views of the mitotic spindle acquired using (a) confocal and (b) 3D STED. The insets show zoomed areas marked with the yellow rectangle. The plots in (c) show intensity cross-sections marked with the yellow line inside the insets. Scale bar in both images are 1µm.
Fig. 5
Fig. 5 The comparison of the mitotic spindle stack obtained with and without aberration correction. Images (a) and (b) show the xy slice obtained at the 4µm distance from the coverslip with and without the aberration correction respectively. (c) and (d) show the corrected and uncorrected XZ views respectively, taken at the location marked with the yellow horizontal lines in (a) and (b). (e) presents the intensity cross-section marked with the vertical yellow lines in (c) and (d). Red plot presents the uncorrected cross-section and blue plot presents the corrected cross-section. Graph (f) presents the amplitudes of the Zernike coefficients that are applied to the SLM.
Fig. 6
Fig. 6 Smooth Manifold Extraction (SME) projection of the stacks acquired using (a) confocal, (b) 2D STED and (c) 3D STED microscope mode with the aberrations corrected. Scale bar in all images is 1µm.

Equations (1)

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Z n m ( r,φ )= R n m ( r ){ sin( mφ )     for    m>0 cos( mφ )   for    m<0 1                  for    m=0