Abstract

Three-dimensional microscopy is mandatory for biological investigation. We describe a stimulated emission depletion selective plane illumination microscope (STED-SPIM) that provides both ease of implementation and an efficient optical slicing. This self-aligned system is based on a single diode-pumped solid-state laser and phase masks made of simple cover glass. A three-fold reduction of the light sheet thickness is achieved together with an enhancement of the sheet uniformity. This method is validated by using fluorescent microspheres and thick slices of fixed and clarified mouse brain to provide an enhanced imaging of Alzheimer’s disease models.

© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Full Article  |  PDF Article
OSA Recommended Articles
STED-SPIM made simple

Teodora Scheul, Irène Wang, and Jean-Claude Vial
Opt. Express 22(25) 30852-30864 (2014)

Fast, super resolution imaging via Bessel-beam stimulated emission depletion microscopy

P. Zhang, P. M. Goodwin, and J. H. Werner
Opt. Express 22(10) 12398-12409 (2014)

Stimulation Emission Depleted Photoacoustic

Seongho Park, Minju Kim, Jean-Claude Vial, and Kwangseuk Kyhm
Opt. Express 27(20) 27841-27850 (2019)

References

  • View by:
  • |
  • |
  • |

  1. M. Ragazzi, S. Piana, C. Longo, F. Castagnetti, M. Foroni, G. Ferrari, G. Gardini, and G. Pellacani, “Fluorescence confocal microscopy for pathologists,” Mod. Pathol. 27(3), 460–471 (2014).
    [Crossref]
  2. P. A. Santi, “Light sheet fluorescence microscopy: a review,” J. Histochem. Cytochem. 59(2), 129–138 (2011).
    [Crossref]
  3. J. Durnin, J. J. Miceli, and J. H. Eberly, “Diffraction-Free Beams,” Phys. Rev. Lett. 58(15), 1499–1501 (1987).
    [Crossref]
  4. F. O. Fahrbach, V. Gurchenkov, K. Alessandri, P. Nassoy, and A. Rohrbach, “Light-sheet microscopy in thick media using scanned Bessel beams and two-photon fluorescence excitation,” Opt. Express 21(11), 13824–13839 (2013).
    [Crossref]
  5. T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Llado, D. E. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
    [Crossref]
  6. B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
    [Crossref]
  7. M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
    [Crossref]
  8. P. Zhang, P. M. Goodwin, and J. H. Werner, “Fast, super resolution imaging via Bessel-beam stimulated emission depletion microscopy,” Opt. Express 22(10), 12398–12409 (2014).
    [Crossref]
  9. C. Gohn-Kreuz and A. Rohrbach, “Light-sheet generation in inhomogeneous media using self-reconstructing beams and the STED-principle,” Opt. Express 24(6), 5855–5865 (2016).
    [Crossref]
  10. P. Hoyer, G. de Medeiros, B. Balázs, N. Norlin, C. Besir, J. Hanne, H.-G. Kräusslich, J. Engelhardt, S. J. Sahl, S. W. Hell, and L. Hufnagel, “Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT,” Proc. Natl. Acad. Sci. 113(13), 3442–3446 (2016).
    [Crossref]
  11. C. Gohn-Kreuz and A. Rohrbach, “Light needles in scattering media using self-reconstructing beams and the STED principle,” Optica 4(9), 1134–1142 (2017).
    [Crossref]
  12. T. Scheul, I. Wang, and J. C. Vial, “STED-SPIM made simple,” Opt. Express 22(25), 30852–30864 (2014).
    [Crossref]
  13. S. W. Hell and J. Wichmann, “Breaking the Diffraction Resolution Limit by Stimulated-Emission - Stimulated-Emission-Depletion Fluorescence Microscopy,” Opt. Lett. 19(11), 780–782 (1994).
    [Crossref]
  14. G. Donnert, C. Eggeling, and S. W. Hell, “Triplet-relaxation microscopy with bunched pulsed excitation,” Photochem. Photobiol. Sci. 8(4), 481–485 (2009).
    [Crossref]
  15. M. C. Chiang, J. C. Garcia, and J. M. Liu, “Depletion Dynamics for Stimulated Emission Depletion (STED) Microscopy,” in 2008 Conference on Lasers and Electro-Optics & Quantum Electronics and Laser Science Conference, Vols 1-9 (IEEE, 2008), pp. 250–251.
  16. K. I. Willig, B. Harke, R. Medda, and S. W. Hell, “STED microscopy with continuous wave beams,” Nat. Methods 4(11), 915–918 (2007).
    [Crossref]
  17. O. E. Olarte, J. Andilla, E. J. Gualda, and P. Loza-Alvarez, “Light-sheet microscopy: a tutorial,” Adv. Opt. Photonics 10(1), 111–179 (2018).
    [Crossref]
  18. H. D. Betz, “An asymmetry method for high precision alignment with laser light,” Appl. Opt. 8(5), 1007–1013 (1969).
    [Crossref]
  19. M. T. Tavassoly, S. R. Hosseini, A. M. Fard, and R. R. Naraghi, “Applications of Fresnel diffraction from the edge of a transparent plate in transmission,” Appl. Opt. 51(30), 7170–7175 (2012).
    [Crossref]
  20. E. Schimitschek, J. Trias, P. Hammond, and R. Atkins, “Laser performance and stability of fluorinated coumarin dyes,” Opt. Commun. 11(4), 352–355 (1974).
    [Crossref]
  21. K. Chung and K. Deisseroth, “CLARITY for mapping the nervous system,” Nat. Methods 10(6), 508–513 (2013).
    [Crossref]
  22. I. Costantini, R. Cicchi, L. Silvestri, F. Vanzi, and F. S. Pavone, “In-vivo and ex-vivo optical clearing methods for biological tissues: review,” Biomed. Opt. Express 10(10), 5251–5267 (2019).
    [Crossref]
  23. R. Blank and D. Mody. 1986. Patent “Stable hydrogen peroxide gels” American Home Products Corporation NY.
  24. M. Reuss, J. Engelhardt, and S. W. Hell, “Birefringent device converts a standard scanning microscope into a STED microscope that also maps molecular orientation,” Opt. Express 18(2), 1049–1058 (2010).
    [Crossref]
  25. S. Saghafi and C. J. R. Sheppard, “Near field and far field of elegantHermite-Gaussian and Laguerre-Gaussian modes,” J. Mod. Opt. 45(10), 1999–2009 (1998).
    [Crossref]
  26. J. Enderlein and F. Pampaloni, “Unified operator approach for deriving Hermite-Gaussian and Laguerre-Gaussian laser modes,” J. Opt. Soc. Am. A 21(8), 1553–1558 (2004).
    [Crossref]
  27. W. E. Klunk, C. J. Xu, R. J. McClure, K. Panchalingam, J. A. Stanley, and J. W. Pettegrew, “Aggregation of beta-amyloid peptide is promoted by membrane phospholipid metabolites elevated in Alzheimer's disease brain,” J. Neurochem. 69(1), 266–272 (2002).
    [Crossref]
  28. L. Nagel-Steger, B. Demeler, W. Meyer-Zaika, K. Hochdorffer, T. Schrader, and D. Willbold, “Modulation of aggregate size- and shape-distributions of the amyloid-beta peptide by a designed beta-sheet breaker,” Eur. Biophys. J. 39(3), 415–422 (2010).
    [Crossref]
  29. L. F. Eng, R. S. Ghirnikar, and Y. L. Lee, “Glial fibrillary acidic protein: GFAP-thirty-one years (1969-2000),” Neurochem. Res. 25(9/10), 1439–1451 (2000).
    [Crossref]
  30. T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
    [Crossref]
  31. B. Harke, J. Keller, C. K. Ullal, V. Westphal, A. Schoenle, and S. W. Hell, “Resolution scaling in STED microscopy,” Opt. Express 16(6), 4154–4162 (2008).
    [Crossref]

2019 (1)

2018 (1)

O. E. Olarte, J. Andilla, E. J. Gualda, and P. Loza-Alvarez, “Light-sheet microscopy: a tutorial,” Adv. Opt. Photonics 10(1), 111–179 (2018).
[Crossref]

2017 (1)

2016 (2)

C. Gohn-Kreuz and A. Rohrbach, “Light-sheet generation in inhomogeneous media using self-reconstructing beams and the STED-principle,” Opt. Express 24(6), 5855–5865 (2016).
[Crossref]

P. Hoyer, G. de Medeiros, B. Balázs, N. Norlin, C. Besir, J. Hanne, H.-G. Kräusslich, J. Engelhardt, S. J. Sahl, S. W. Hell, and L. Hufnagel, “Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT,” Proc. Natl. Acad. Sci. 113(13), 3442–3446 (2016).
[Crossref]

2014 (5)

P. Zhang, P. M. Goodwin, and J. H. Werner, “Fast, super resolution imaging via Bessel-beam stimulated emission depletion microscopy,” Opt. Express 22(10), 12398–12409 (2014).
[Crossref]

M. Ragazzi, S. Piana, C. Longo, F. Castagnetti, M. Foroni, G. Ferrari, G. Gardini, and G. Pellacani, “Fluorescence confocal microscopy for pathologists,” Mod. Pathol. 27(3), 460–471 (2014).
[Crossref]

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Llado, D. E. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

T. Scheul, I. Wang, and J. C. Vial, “STED-SPIM made simple,” Opt. Express 22(25), 30852–30864 (2014).
[Crossref]

2013 (2)

2012 (1)

2011 (3)

M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
[Crossref]

P. A. Santi, “Light sheet fluorescence microscopy: a review,” J. Histochem. Cytochem. 59(2), 129–138 (2011).
[Crossref]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref]

2010 (2)

L. Nagel-Steger, B. Demeler, W. Meyer-Zaika, K. Hochdorffer, T. Schrader, and D. Willbold, “Modulation of aggregate size- and shape-distributions of the amyloid-beta peptide by a designed beta-sheet breaker,” Eur. Biophys. J. 39(3), 415–422 (2010).
[Crossref]

M. Reuss, J. Engelhardt, and S. W. Hell, “Birefringent device converts a standard scanning microscope into a STED microscope that also maps molecular orientation,” Opt. Express 18(2), 1049–1058 (2010).
[Crossref]

2009 (1)

G. Donnert, C. Eggeling, and S. W. Hell, “Triplet-relaxation microscopy with bunched pulsed excitation,” Photochem. Photobiol. Sci. 8(4), 481–485 (2009).
[Crossref]

2008 (1)

2007 (1)

K. I. Willig, B. Harke, R. Medda, and S. W. Hell, “STED microscopy with continuous wave beams,” Nat. Methods 4(11), 915–918 (2007).
[Crossref]

2004 (1)

2002 (1)

W. E. Klunk, C. J. Xu, R. J. McClure, K. Panchalingam, J. A. Stanley, and J. W. Pettegrew, “Aggregation of beta-amyloid peptide is promoted by membrane phospholipid metabolites elevated in Alzheimer's disease brain,” J. Neurochem. 69(1), 266–272 (2002).
[Crossref]

2000 (1)

L. F. Eng, R. S. Ghirnikar, and Y. L. Lee, “Glial fibrillary acidic protein: GFAP-thirty-one years (1969-2000),” Neurochem. Res. 25(9/10), 1439–1451 (2000).
[Crossref]

1998 (1)

S. Saghafi and C. J. R. Sheppard, “Near field and far field of elegantHermite-Gaussian and Laguerre-Gaussian modes,” J. Mod. Opt. 45(10), 1999–2009 (1998).
[Crossref]

1994 (1)

1987 (1)

J. Durnin, J. J. Miceli, and J. H. Eberly, “Diffraction-Free Beams,” Phys. Rev. Lett. 58(15), 1499–1501 (1987).
[Crossref]

1974 (1)

E. Schimitschek, J. Trias, P. Hammond, and R. Atkins, “Laser performance and stability of fluorinated coumarin dyes,” Opt. Commun. 11(4), 352–355 (1974).
[Crossref]

1969 (1)

Alessandri, K.

Andilla, J.

O. E. Olarte, J. Andilla, E. J. Gualda, and P. Loza-Alvarez, “Light-sheet microscopy: a tutorial,” Adv. Opt. Photonics 10(1), 111–179 (2018).
[Crossref]

Atkins, R.

E. Schimitschek, J. Trias, P. Hammond, and R. Atkins, “Laser performance and stability of fluorinated coumarin dyes,” Opt. Commun. 11(4), 352–355 (1974).
[Crossref]

Balázs, B.

P. Hoyer, G. de Medeiros, B. Balázs, N. Norlin, C. Besir, J. Hanne, H.-G. Kräusslich, J. Engelhardt, S. J. Sahl, S. W. Hell, and L. Hufnagel, “Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT,” Proc. Natl. Acad. Sci. 113(13), 3442–3446 (2016).
[Crossref]

Bembenek, J. N.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Besir, C.

P. Hoyer, G. de Medeiros, B. Balázs, N. Norlin, C. Besir, J. Hanne, H.-G. Kräusslich, J. Engelhardt, S. J. Sahl, S. W. Hell, and L. Hufnagel, “Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT,” Proc. Natl. Acad. Sci. 113(13), 3442–3446 (2016).
[Crossref]

Betz, H. D.

Betzig, E.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref]

Blank, R.

R. Blank and D. Mody. 1986. Patent “Stable hydrogen peroxide gels” American Home Products Corporation NY.

Bohme, R.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Castagnetti, F.

M. Ragazzi, S. Piana, C. Longo, F. Castagnetti, M. Foroni, G. Ferrari, G. Gardini, and G. Pellacani, “Fluorescence confocal microscopy for pathologists,” Mod. Pathol. 27(3), 460–471 (2014).
[Crossref]

Chen, B. C.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Chiang, M. C.

M. C. Chiang, J. C. Garcia, and J. M. Liu, “Depletion Dynamics for Stimulated Emission Depletion (STED) Microscopy,” in 2008 Conference on Lasers and Electro-Optics & Quantum Electronics and Laser Science Conference, Vols 1-9 (IEEE, 2008), pp. 250–251.

Chung, K.

K. Chung and K. Deisseroth, “CLARITY for mapping the nervous system,” Nat. Methods 10(6), 508–513 (2013).
[Crossref]

Cicchi, R.

Cizmar, T.

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Llado, D. E. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

Coll-Llado, C.

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Llado, D. E. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

Costantini, I.

Dalgarno, H. I.

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Llado, D. E. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

Davidson, M. W.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref]

de Medeiros, G.

P. Hoyer, G. de Medeiros, B. Balázs, N. Norlin, C. Besir, J. Hanne, H.-G. Kräusslich, J. Engelhardt, S. J. Sahl, S. W. Hell, and L. Hufnagel, “Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT,” Proc. Natl. Acad. Sci. 113(13), 3442–3446 (2016).
[Crossref]

Deisseroth, K.

K. Chung and K. Deisseroth, “CLARITY for mapping the nervous system,” Nat. Methods 10(6), 508–513 (2013).
[Crossref]

Demeler, B.

L. Nagel-Steger, B. Demeler, W. Meyer-Zaika, K. Hochdorffer, T. Schrader, and D. Willbold, “Modulation of aggregate size- and shape-distributions of the amyloid-beta peptide by a designed beta-sheet breaker,” Eur. Biophys. J. 39(3), 415–422 (2010).
[Crossref]

Dholakia, K.

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Llado, D. E. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

Donnert, G.

G. Donnert, C. Eggeling, and S. W. Hell, “Triplet-relaxation microscopy with bunched pulsed excitation,” Photochem. Photobiol. Sci. 8(4), 481–485 (2009).
[Crossref]

Durnin, J.

J. Durnin, J. J. Miceli, and J. H. Eberly, “Diffraction-Free Beams,” Phys. Rev. Lett. 58(15), 1499–1501 (1987).
[Crossref]

Eberly, J. H.

J. Durnin, J. J. Miceli, and J. H. Eberly, “Diffraction-Free Beams,” Phys. Rev. Lett. 58(15), 1499–1501 (1987).
[Crossref]

Eggeling, C.

G. Donnert, C. Eggeling, and S. W. Hell, “Triplet-relaxation microscopy with bunched pulsed excitation,” Photochem. Photobiol. Sci. 8(4), 481–485 (2009).
[Crossref]

Enderlein, J.

Eng, L. F.

L. F. Eng, R. S. Ghirnikar, and Y. L. Lee, “Glial fibrillary acidic protein: GFAP-thirty-one years (1969-2000),” Neurochem. Res. 25(9/10), 1439–1451 (2000).
[Crossref]

Engelhardt, J.

P. Hoyer, G. de Medeiros, B. Balázs, N. Norlin, C. Besir, J. Hanne, H.-G. Kräusslich, J. Engelhardt, S. J. Sahl, S. W. Hell, and L. Hufnagel, “Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT,” Proc. Natl. Acad. Sci. 113(13), 3442–3446 (2016).
[Crossref]

M. Reuss, J. Engelhardt, and S. W. Hell, “Birefringent device converts a standard scanning microscope into a STED microscope that also maps molecular orientation,” Opt. Express 18(2), 1049–1058 (2010).
[Crossref]

English, B. P.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Ermolayev, V.

M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
[Crossref]

Fahrbach, F. O.

Fard, A. M.

Ferrari, G.

M. Ragazzi, S. Piana, C. Longo, F. Castagnetti, M. Foroni, G. Ferrari, G. Gardini, and G. Pellacani, “Fluorescence confocal microscopy for pathologists,” Mod. Pathol. 27(3), 460–471 (2014).
[Crossref]

Ferrier, D. E.

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Llado, D. E. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

Foroni, M.

M. Ragazzi, S. Piana, C. Longo, F. Castagnetti, M. Foroni, G. Ferrari, G. Gardini, and G. Pellacani, “Fluorescence confocal microscopy for pathologists,” Mod. Pathol. 27(3), 460–471 (2014).
[Crossref]

Friedrich, M.

M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
[Crossref]

Fritz-Laylin, L.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Galbraith, C. G.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref]

Galbraith, J. A.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref]

Gan, Q.

M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
[Crossref]

Gao, L.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref]

Garcia, J. C.

M. C. Chiang, J. C. Garcia, and J. M. Liu, “Depletion Dynamics for Stimulated Emission Depletion (STED) Microscopy,” in 2008 Conference on Lasers and Electro-Optics & Quantum Electronics and Laser Science Conference, Vols 1-9 (IEEE, 2008), pp. 250–251.

Gardini, G.

M. Ragazzi, S. Piana, C. Longo, F. Castagnetti, M. Foroni, G. Ferrari, G. Gardini, and G. Pellacani, “Fluorescence confocal microscopy for pathologists,” Mod. Pathol. 27(3), 460–471 (2014).
[Crossref]

Ghirnikar, R. S.

L. F. Eng, R. S. Ghirnikar, and Y. L. Lee, “Glial fibrillary acidic protein: GFAP-thirty-one years (1969-2000),” Neurochem. Res. 25(9/10), 1439–1451 (2000).
[Crossref]

Gohn-Kreuz, C.

Goodwin, P. M.

Grill, S. W.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Gualda, E. J.

O. E. Olarte, J. Andilla, E. J. Gualda, and P. Loza-Alvarez, “Light-sheet microscopy: a tutorial,” Adv. Opt. Photonics 10(1), 111–179 (2018).
[Crossref]

Gunn-Moore, F. J.

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Llado, D. E. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

Gurchenkov, V.

Hammer, J. A.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Hammond, P.

E. Schimitschek, J. Trias, P. Hammond, and R. Atkins, “Laser performance and stability of fluorinated coumarin dyes,” Opt. Commun. 11(4), 352–355 (1974).
[Crossref]

Hanne, J.

P. Hoyer, G. de Medeiros, B. Balázs, N. Norlin, C. Besir, J. Hanne, H.-G. Kräusslich, J. Engelhardt, S. J. Sahl, S. W. Hell, and L. Hufnagel, “Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT,” Proc. Natl. Acad. Sci. 113(13), 3442–3446 (2016).
[Crossref]

Harke, B.

B. Harke, J. Keller, C. K. Ullal, V. Westphal, A. Schoenle, and S. W. Hell, “Resolution scaling in STED microscopy,” Opt. Express 16(6), 4154–4162 (2008).
[Crossref]

K. I. Willig, B. Harke, R. Medda, and S. W. Hell, “STED microscopy with continuous wave beams,” Nat. Methods 4(11), 915–918 (2007).
[Crossref]

Harms, G. S.

M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
[Crossref]

Hell, S. W.

P. Hoyer, G. de Medeiros, B. Balázs, N. Norlin, C. Besir, J. Hanne, H.-G. Kräusslich, J. Engelhardt, S. J. Sahl, S. W. Hell, and L. Hufnagel, “Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT,” Proc. Natl. Acad. Sci. 113(13), 3442–3446 (2016).
[Crossref]

M. Reuss, J. Engelhardt, and S. W. Hell, “Birefringent device converts a standard scanning microscope into a STED microscope that also maps molecular orientation,” Opt. Express 18(2), 1049–1058 (2010).
[Crossref]

G. Donnert, C. Eggeling, and S. W. Hell, “Triplet-relaxation microscopy with bunched pulsed excitation,” Photochem. Photobiol. Sci. 8(4), 481–485 (2009).
[Crossref]

B. Harke, J. Keller, C. K. Ullal, V. Westphal, A. Schoenle, and S. W. Hell, “Resolution scaling in STED microscopy,” Opt. Express 16(6), 4154–4162 (2008).
[Crossref]

K. I. Willig, B. Harke, R. Medda, and S. W. Hell, “STED microscopy with continuous wave beams,” Nat. Methods 4(11), 915–918 (2007).
[Crossref]

S. W. Hell and J. Wichmann, “Breaking the Diffraction Resolution Limit by Stimulated-Emission - Stimulated-Emission-Depletion Fluorescence Microscopy,” Opt. Lett. 19(11), 780–782 (1994).
[Crossref]

Hochdorffer, K.

L. Nagel-Steger, B. Demeler, W. Meyer-Zaika, K. Hochdorffer, T. Schrader, and D. Willbold, “Modulation of aggregate size- and shape-distributions of the amyloid-beta peptide by a designed beta-sheet breaker,” Eur. Biophys. J. 39(3), 415–422 (2010).
[Crossref]

Hosseini, S. R.

Hoyer, P.

P. Hoyer, G. de Medeiros, B. Balázs, N. Norlin, C. Besir, J. Hanne, H.-G. Kräusslich, J. Engelhardt, S. J. Sahl, S. W. Hell, and L. Hufnagel, “Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT,” Proc. Natl. Acad. Sci. 113(13), 3442–3446 (2016).
[Crossref]

Hufnagel, L.

P. Hoyer, G. de Medeiros, B. Balázs, N. Norlin, C. Besir, J. Hanne, H.-G. Kräusslich, J. Engelhardt, S. J. Sahl, S. W. Hell, and L. Hufnagel, “Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT,” Proc. Natl. Acad. Sci. 113(13), 3442–3446 (2016).
[Crossref]

Janetopoulos, C.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Keller, J.

Kiehart, D. P.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Klunk, W. E.

W. E. Klunk, C. J. Xu, R. J. McClure, K. Panchalingam, J. A. Stanley, and J. W. Pettegrew, “Aggregation of beta-amyloid peptide is promoted by membrane phospholipid metabolites elevated in Alzheimer's disease brain,” J. Neurochem. 69(1), 266–272 (2002).
[Crossref]

Kräusslich, H.-G.

P. Hoyer, G. de Medeiros, B. Balázs, N. Norlin, C. Besir, J. Hanne, H.-G. Kräusslich, J. Engelhardt, S. J. Sahl, S. W. Hell, and L. Hufnagel, “Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT,” Proc. Natl. Acad. Sci. 113(13), 3442–3446 (2016).
[Crossref]

Lee, Y. L.

L. F. Eng, R. S. Ghirnikar, and Y. L. Lee, “Glial fibrillary acidic protein: GFAP-thirty-one years (1969-2000),” Neurochem. Res. 25(9/10), 1439–1451 (2000).
[Crossref]

Legant, W. R.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Lippincott-Schwartz, J.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Liu, J. M.

M. C. Chiang, J. C. Garcia, and J. M. Liu, “Depletion Dynamics for Stimulated Emission Depletion (STED) Microscopy,” in 2008 Conference on Lasers and Electro-Optics & Quantum Electronics and Laser Science Conference, Vols 1-9 (IEEE, 2008), pp. 250–251.

Liu, Z.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Longo, C.

M. Ragazzi, S. Piana, C. Longo, F. Castagnetti, M. Foroni, G. Ferrari, G. Gardini, and G. Pellacani, “Fluorescence confocal microscopy for pathologists,” Mod. Pathol. 27(3), 460–471 (2014).
[Crossref]

Loza-Alvarez, P.

O. E. Olarte, J. Andilla, E. J. Gualda, and P. Loza-Alvarez, “Light-sheet microscopy: a tutorial,” Adv. Opt. Photonics 10(1), 111–179 (2018).
[Crossref]

McClure, R. J.

W. E. Klunk, C. J. Xu, R. J. McClure, K. Panchalingam, J. A. Stanley, and J. W. Pettegrew, “Aggregation of beta-amyloid peptide is promoted by membrane phospholipid metabolites elevated in Alzheimer's disease brain,” J. Neurochem. 69(1), 266–272 (2002).
[Crossref]

Medda, R.

K. I. Willig, B. Harke, R. Medda, and S. W. Hell, “STED microscopy with continuous wave beams,” Nat. Methods 4(11), 915–918 (2007).
[Crossref]

Meyer-Zaika, W.

L. Nagel-Steger, B. Demeler, W. Meyer-Zaika, K. Hochdorffer, T. Schrader, and D. Willbold, “Modulation of aggregate size- and shape-distributions of the amyloid-beta peptide by a designed beta-sheet breaker,” Eur. Biophys. J. 39(3), 415–422 (2010).
[Crossref]

Miceli, J. J.

J. Durnin, J. J. Miceli, and J. H. Eberly, “Diffraction-Free Beams,” Phys. Rev. Lett. 58(15), 1499–1501 (1987).
[Crossref]

Milkie, D. E.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref]

Mimori-Kiyosue, Y.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Mitchell, D. M.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Mody, D.

R. Blank and D. Mody. 1986. Patent “Stable hydrogen peroxide gels” American Home Products Corporation NY.

Mullins, R. D.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Nagel-Steger, L.

L. Nagel-Steger, B. Demeler, W. Meyer-Zaika, K. Hochdorffer, T. Schrader, and D. Willbold, “Modulation of aggregate size- and shape-distributions of the amyloid-beta peptide by a designed beta-sheet breaker,” Eur. Biophys. J. 39(3), 415–422 (2010).
[Crossref]

Naraghi, R. R.

Nassoy, P.

Norlin, N.

P. Hoyer, G. de Medeiros, B. Balázs, N. Norlin, C. Besir, J. Hanne, H.-G. Kräusslich, J. Engelhardt, S. J. Sahl, S. W. Hell, and L. Hufnagel, “Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT,” Proc. Natl. Acad. Sci. 113(13), 3442–3446 (2016).
[Crossref]

Nylk, J.

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Llado, D. E. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

Olarte, O. E.

O. E. Olarte, J. Andilla, E. J. Gualda, and P. Loza-Alvarez, “Light-sheet microscopy: a tutorial,” Adv. Opt. Photonics 10(1), 111–179 (2018).
[Crossref]

Pampaloni, F.

Panchalingam, K.

W. E. Klunk, C. J. Xu, R. J. McClure, K. Panchalingam, J. A. Stanley, and J. W. Pettegrew, “Aggregation of beta-amyloid peptide is promoted by membrane phospholipid metabolites elevated in Alzheimer's disease brain,” J. Neurochem. 69(1), 266–272 (2002).
[Crossref]

Pavone, F. S.

Pellacani, G.

M. Ragazzi, S. Piana, C. Longo, F. Castagnetti, M. Foroni, G. Ferrari, G. Gardini, and G. Pellacani, “Fluorescence confocal microscopy for pathologists,” Mod. Pathol. 27(3), 460–471 (2014).
[Crossref]

Pettegrew, J. W.

W. E. Klunk, C. J. Xu, R. J. McClure, K. Panchalingam, J. A. Stanley, and J. W. Pettegrew, “Aggregation of beta-amyloid peptide is promoted by membrane phospholipid metabolites elevated in Alzheimer's disease brain,” J. Neurochem. 69(1), 266–272 (2002).
[Crossref]

Piana, S.

M. Ragazzi, S. Piana, C. Longo, F. Castagnetti, M. Foroni, G. Ferrari, G. Gardini, and G. Pellacani, “Fluorescence confocal microscopy for pathologists,” Mod. Pathol. 27(3), 460–471 (2014).
[Crossref]

Planchon, T. A.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref]

Ragazzi, M.

M. Ragazzi, S. Piana, C. Longo, F. Castagnetti, M. Foroni, G. Ferrari, G. Gardini, and G. Pellacani, “Fluorescence confocal microscopy for pathologists,” Mod. Pathol. 27(3), 460–471 (2014).
[Crossref]

Reuss, M.

Reymann, A. C.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Ritter, A. T.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Rohrbach, A.

Romero, D. P.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Saghafi, S.

S. Saghafi and C. J. R. Sheppard, “Near field and far field of elegantHermite-Gaussian and Laguerre-Gaussian modes,” J. Mod. Opt. 45(10), 1999–2009 (1998).
[Crossref]

Sahl, S. J.

P. Hoyer, G. de Medeiros, B. Balázs, N. Norlin, C. Besir, J. Hanne, H.-G. Kräusslich, J. Engelhardt, S. J. Sahl, S. W. Hell, and L. Hufnagel, “Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT,” Proc. Natl. Acad. Sci. 113(13), 3442–3446 (2016).
[Crossref]

Santi, P. A.

P. A. Santi, “Light sheet fluorescence microscopy: a review,” J. Histochem. Cytochem. 59(2), 129–138 (2011).
[Crossref]

Scheul, T.

Schimitschek, E.

E. Schimitschek, J. Trias, P. Hammond, and R. Atkins, “Laser performance and stability of fluorinated coumarin dyes,” Opt. Commun. 11(4), 352–355 (1974).
[Crossref]

Schoenle, A.

Schrader, T.

L. Nagel-Steger, B. Demeler, W. Meyer-Zaika, K. Hochdorffer, T. Schrader, and D. Willbold, “Modulation of aggregate size- and shape-distributions of the amyloid-beta peptide by a designed beta-sheet breaker,” Eur. Biophys. J. 39(3), 415–422 (2010).
[Crossref]

Seydoux, G.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Shao, L.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Sheppard, C. J. R.

S. Saghafi and C. J. R. Sheppard, “Near field and far field of elegantHermite-Gaussian and Laguerre-Gaussian modes,” J. Mod. Opt. 45(10), 1999–2009 (1998).
[Crossref]

Silvestri, L.

Stanley, J. A.

W. E. Klunk, C. J. Xu, R. J. McClure, K. Panchalingam, J. A. Stanley, and J. W. Pettegrew, “Aggregation of beta-amyloid peptide is promoted by membrane phospholipid metabolites elevated in Alzheimer's disease brain,” J. Neurochem. 69(1), 266–272 (2002).
[Crossref]

Tavassoly, M. T.

Trias, J.

E. Schimitschek, J. Trias, P. Hammond, and R. Atkins, “Laser performance and stability of fluorinated coumarin dyes,” Opt. Commun. 11(4), 352–355 (1974).
[Crossref]

Tulu, U. S.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Ullal, C. K.

Vanzi, F.

Vettenburg, T.

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Llado, D. E. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

Vial, J. C.

Wang, I.

Wang, J. T.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Wang, K.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Werner, J. H.

Westphal, V.

Wichmann, J.

Willbold, D.

L. Nagel-Steger, B. Demeler, W. Meyer-Zaika, K. Hochdorffer, T. Schrader, and D. Willbold, “Modulation of aggregate size- and shape-distributions of the amyloid-beta peptide by a designed beta-sheet breaker,” Eur. Biophys. J. 39(3), 415–422 (2010).
[Crossref]

Willig, K. I.

K. I. Willig, B. Harke, R. Medda, and S. W. Hell, “STED microscopy with continuous wave beams,” Nat. Methods 4(11), 915–918 (2007).
[Crossref]

Wu, X. F. S.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Xu, C. J.

W. E. Klunk, C. J. Xu, R. J. McClure, K. Panchalingam, J. A. Stanley, and J. W. Pettegrew, “Aggregation of beta-amyloid peptide is promoted by membrane phospholipid metabolites elevated in Alzheimer's disease brain,” J. Neurochem. 69(1), 266–272 (2002).
[Crossref]

Zhang, P.

Adv. Opt. Photonics (1)

O. E. Olarte, J. Andilla, E. J. Gualda, and P. Loza-Alvarez, “Light-sheet microscopy: a tutorial,” Adv. Opt. Photonics 10(1), 111–179 (2018).
[Crossref]

Appl. Opt. (2)

Biomed. Opt. Express (1)

Biophys. J. (1)

M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
[Crossref]

Eur. Biophys. J. (1)

L. Nagel-Steger, B. Demeler, W. Meyer-Zaika, K. Hochdorffer, T. Schrader, and D. Willbold, “Modulation of aggregate size- and shape-distributions of the amyloid-beta peptide by a designed beta-sheet breaker,” Eur. Biophys. J. 39(3), 415–422 (2010).
[Crossref]

J. Histochem. Cytochem. (1)

P. A. Santi, “Light sheet fluorescence microscopy: a review,” J. Histochem. Cytochem. 59(2), 129–138 (2011).
[Crossref]

J. Mod. Opt. (1)

S. Saghafi and C. J. R. Sheppard, “Near field and far field of elegantHermite-Gaussian and Laguerre-Gaussian modes,” J. Mod. Opt. 45(10), 1999–2009 (1998).
[Crossref]

J. Neurochem. (1)

W. E. Klunk, C. J. Xu, R. J. McClure, K. Panchalingam, J. A. Stanley, and J. W. Pettegrew, “Aggregation of beta-amyloid peptide is promoted by membrane phospholipid metabolites elevated in Alzheimer's disease brain,” J. Neurochem. 69(1), 266–272 (2002).
[Crossref]

J. Opt. Soc. Am. A (1)

Mod. Pathol. (1)

M. Ragazzi, S. Piana, C. Longo, F. Castagnetti, M. Foroni, G. Ferrari, G. Gardini, and G. Pellacani, “Fluorescence confocal microscopy for pathologists,” Mod. Pathol. 27(3), 460–471 (2014).
[Crossref]

Nat. Methods (4)

K. I. Willig, B. Harke, R. Medda, and S. W. Hell, “STED microscopy with continuous wave beams,” Nat. Methods 4(11), 915–918 (2007).
[Crossref]

K. Chung and K. Deisseroth, “CLARITY for mapping the nervous system,” Nat. Methods 10(6), 508–513 (2013).
[Crossref]

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Llado, D. E. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref]

Neurochem. Res. (1)

L. F. Eng, R. S. Ghirnikar, and Y. L. Lee, “Glial fibrillary acidic protein: GFAP-thirty-one years (1969-2000),” Neurochem. Res. 25(9/10), 1439–1451 (2000).
[Crossref]

Opt. Commun. (1)

E. Schimitschek, J. Trias, P. Hammond, and R. Atkins, “Laser performance and stability of fluorinated coumarin dyes,” Opt. Commun. 11(4), 352–355 (1974).
[Crossref]

Opt. Express (6)

Opt. Lett. (1)

Optica (1)

Photochem. Photobiol. Sci. (1)

G. Donnert, C. Eggeling, and S. W. Hell, “Triplet-relaxation microscopy with bunched pulsed excitation,” Photochem. Photobiol. Sci. 8(4), 481–485 (2009).
[Crossref]

Phys. Rev. Lett. (1)

J. Durnin, J. J. Miceli, and J. H. Eberly, “Diffraction-Free Beams,” Phys. Rev. Lett. 58(15), 1499–1501 (1987).
[Crossref]

Proc. Natl. Acad. Sci. (1)

P. Hoyer, G. de Medeiros, B. Balázs, N. Norlin, C. Besir, J. Hanne, H.-G. Kräusslich, J. Engelhardt, S. J. Sahl, S. W. Hell, and L. Hufnagel, “Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT,” Proc. Natl. Acad. Sci. 113(13), 3442–3446 (2016).
[Crossref]

Science (1)

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Other (2)

M. C. Chiang, J. C. Garcia, and J. M. Liu, “Depletion Dynamics for Stimulated Emission Depletion (STED) Microscopy,” in 2008 Conference on Lasers and Electro-Optics & Quantum Electronics and Laser Science Conference, Vols 1-9 (IEEE, 2008), pp. 250–251.

R. Blank and D. Mody. 1986. Patent “Stable hydrogen peroxide gels” American Home Products Corporation NY.

Cited By

OSA participates in Crossref's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (8)

Fig. 1.
Fig. 1. (a and b respectively) Images of the fluorescence trace shown schematically with excitation (355 nm) alone and with donut-shaped STED beam (532 nm) added. (c) Experimental measurements of the beam width as a function of axial position, for the excitation beam alone (blue) and with the addition of a STED beam (from Ref. [12]). The red dotted curve is a simulation for a Gaussian beam having a beam waist identical to the experimental beam with STED (green curve)
Fig. 2.
Fig. 2. (a) The UV-Vis laser tandem based on harrmonic generation (b) The all-visible laser tandem pumped by a 532 nm laser. The cavity is defined by the surface of the dye cell. The red output beam is collinear and synchronized with the 532 nm pulses. (c) The typical spectrum of the output of the all visible laser tandem when the dye used is the Pyrromethene 650. (d) Scheme of the experimental setup for STED-SPIM. The sheet produced by the oscillating mirror is in the horizontal xy plane and the sample is translated in the z direction.
Fig. 3.
Fig. 3. (a) and (b) Type 1 and Type 2 phase masks, respectively, adapted for STED-SPIM and dichroic at 532-355 nm. The arrows represent the polarisation direction of the beam. (c) The image of the transmitted 532 nm non-scanned beam is taken in the near field. (d) The image of the same 532 nm beam but in the far field ie at the focus of a lens, the curve is its light intensity profile in the Z direction. The images are obtained with the Type 2 mask but the same result is obtained with the Type 1 mask. We have verified on a set of images taken at 355 nm (not shown here) that the beam is unaffected by these masks. The transmitted beams travelling along the y direction are scanned along the x direction to form the light sheet.
Fig. 4.
Fig. 4. Fluorescent traces in a Rhodamine dye solution excited by a 532 nm beam, focused by a 0.05 NA lens, without (a) and with (b) the two halves phase mask. (c) Measurement of the full width at half maximum of the beam, without mask, and the distance between the maxima of the two sub beams, when the mask is interposed, as a function of the distance of propagation from the focus.
Fig. 5.
Fig. 5. Fluorescence light sheets in a Rhodamine dye cell excited at 532 nm and depleted at 600 nm : (a) low focusing, (b) high focusing with their respective beam waist section (c) and (e). The plot (d) is the fluorescence beam section as a function of the distance from focus measured on (b). Low focusing is obtained with a 150 mm focal length (NA around 0.025) while tighter focusing is obtained by a 40 mm focal length (NA around 0.1).
Fig. 6.
Fig. 6. (a). The Z projection (Average intensity) of a 25 µm thick stack of 100 images of fluorescent 0.4-0.6 µm diameter fluorescent microspheres embedded in a pluronic gel excited at 355 nm with stimulation depletion at 532 nm. (b). The X projection of the orthogonal views of the stack with STED. (c). The X projection of the orthogonal view for the same sample but without STED. (d). The Z axis profile of a set of microspheres measured on the stack under stimulation depletion, the microspheres are numbered from 1 to 11. (e) The Z-axis profile of a particular microsphere (surrounded in the image (a)) with and without depletion. The focusing lens has a 0.1 NA.
Fig. 7.
Fig. 7. The Z profile of two contacted microspheres along the Z direction. (a). A zoom of the zone delimited by a square in Fig. 6(a). (b) The orthogonal view of the stack without STED. (c) The orthogonal view of the stack with STED. (d) The Z-profile along the yellow lines in (b) and (c).
Fig. 8.
Fig. 8. Images at various magnification of a block of 12 months old cleared AD mouse (APP:PS1-21 lines) brain embedded in a pluronic gel, the astrocytes are anti-GFAP/CY3 stained (a) Z projection of a 100 µm thick stack (2.2mm×1.8mm). The bright zones are amyloid plaques. The imaging objective (×4) has a NA=0.15 giving a lateral resolution of about 2µm. The focal length of the focusing lens for illumination is 100 mm giving also a super-resolved sheet thickness of ∼2µm. (b) An optical slice taken at 40 µm below the surface. The water-immersion objective (×10) for imaging has a NA=0.3 giving a lateral resolution of about 1µm. The focal length of the focusing lens for illumination is 50 mm giving a super-resolved sheet thickness around 1µm. (c) Z projection of 10 optical slices (0.1 µm thick) taken at 10 µm below the surface. The water-immersion objective (×40) for imaging has a NA=0.5 giving a lateral resolution around 0.5µm. The focal length of the focusing lens for illumination is 25 mm giving also a super-resolved sheet thickness around 0.5 µm.

Metrics