Abstract

Self-interference digital holographic microscopy (DHM) has been found particular suitable for simplified quantitative phase imaging of living cells. However, a main drawback of the self-interference DHM principle are scattering patterns that are induced by the coherent nature of the laser light which affect the resolution for detection of optical path length changes. We present a simple and efficient technique for the reduction of coherent disturbances in quantitative phase images. Therefore, amplitude and phase of the sample illumination are modulated by an electrically focus tunable lens. The proposed method is in particular convenient with the self-interference DHM concept. Results from the characterization of the method show that a reduction of coherence induced disturbances up to 70 percent can be achieved. Finally, the performance for enhanced quantitative imaging of living cells is demonstrated.

© 2014 Optical Society of America

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References

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    [Crossref] [PubMed]

2014 (5)

2013 (4)

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
[Crossref]

B. Bhaduri, K. Tangella, and G. Popescu, “Fourier phase microscopy with white light,” Biomed. Opt. Express 4(8), 1434–1441 (2013).
[Crossref] [PubMed]

P. Girshovitz and N. T. Shaked, “Compact and portable low-coherence interferometer with off-axis geometry for quantitative phase microscopy and nanoscopy,” Opt. Express 21(5), 5701–5714 (2013).
[Crossref] [PubMed]

P. Marquet, C. Depeursinge, and P. J. Magistretti, “Exploring Neural Cell Dynamics with Digital Holographic Microscopy,” Annu. Rev. Biomed. Eng. 15(1), 407–431 (2013).
[Crossref] [PubMed]

2012 (2)

B. Bhaduri, H. Pham, M. Mir, and G. Popescu, “Diffraction phase microscopy with white light,” Opt. Lett. 37(6), 1094–1096 (2012).
[Crossref] [PubMed]

S. Kosmeier, P. Langehanenberg, G. von Bally, and B. Kemper, “Reduction of parasitic interferences in digital holographic microscopy by numerically decreased coherence length,” Appl. Phys. B 106(1), 107–115 (2012).
[Crossref]

2011 (4)

2010 (2)

2009 (4)

M. Debailleul, V. Georges, B. Simon, R. Morin, and O. Haeberlé, “High-resolution three-dimensional tomographic diffractive microscopy of transparent inorganic and biological samples,” Opt. Lett. 34(1), 79–81 (2009).
[Crossref] [PubMed]

P. Bon, G. Maucort, B. Wattellier, and S. Monneret, “Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells,” Opt. Express 17(15), 13080–13094 (2009).
[Crossref] [PubMed]

B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
[Crossref] [PubMed]

B. Kemper, S. Kosmeier, P. Langehanenberg, S. Przibilla, C. Remmersmann, S. Stürwald, and G. von Bally, “Application of 3D tracking, LED illumination and multi-wavelength techniques for quantitative cell analysis in digital holographic microscopy,” Proc. SPIE 7184, 71840R (2009).
[Crossref]

2008 (3)

B. Kemper, S. Stürwald, C. Remmersmann, P. Langehanenberg, and G. von Bally, “Characterisation of light emitting diodes (LEDs) for application in digital holographic microscopy for inspection of micro and nanostructured surfaces,” Opt. Lasers Eng. 46(7), 499–507 (2008).
[Crossref]

B. Kemper and G. von Bally, “Digital holographic microscopy for live cell applications and technical inspection,” Appl. Opt. 47(4), A52–A61 (2008).
[Crossref] [PubMed]

X. Kang, “An effective method for reducing speckle noise in digital holography,” Chin. Opt. Lett. 6(2), 100–103 (2008).
[Crossref]

2007 (2)

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods 4(9), 717–719 (2007).
[Crossref] [PubMed]

B. Kemper, S. Kosmeier, P. Langehanenberg, G. von Bally, I. Bredebusch, W. Domschke, and J. Schnekenburger, “Integral refractive index determination of living suspension cells by multi focus digital holographic phase contrast microscopy,” J. Biomed. Opt. 12(5), 054009 (2007).
[Crossref] [PubMed]

2006 (2)

A. E. Carpenter, T. R. Jones, M. R. Lamprecht, C. Clarke, I. H. Kang, O. Friman, D. A. Guertin, J. H. Chang, R. A. Lindquist, J. Moffat, P. Golland, and D. M. Sabatini, “CellProfiler: image analysis software for identifying and quantifying cell phenotypes,” Genome Biol. 7(10), R100 (2006).
[Crossref] [PubMed]

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, “Investigation of living pancreas tumor cells by digital holographic microscopy,” J. Biomed. Opt. 11(3), 034005 (2006).
[Crossref] [PubMed]

2004 (1)

2003 (1)

B. Kemper, J. Kandulla, D. Dirksen, and G. von Bally, “Optimization of spatial phase shifting in endoscopic electronic-speckle-pattern-interferometry,” Opt. Commun. 217(1-6), 151–160 (2003).
[Crossref]

1999 (2)

1952 (1)

R. Barer, “Interference microscopy and mass determination,” Nature 169(4296), 366–367 (1952).
[Crossref] [PubMed]

Aknoun, S.

Badizadegan, K.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods 4(9), 717–719 (2007).
[Crossref] [PubMed]

Bailleul, J.

Balduzzi, D.

Barer, R.

R. Barer, “Interference microscopy and mass determination,” Nature 169(4296), 366–367 (1952).
[Crossref] [PubMed]

Bettenworth, D.

D. Bettenworth, P. Lenz, P. Krausewitz, M. Brückner, S. Ketelhut, D. Domagk, and B. Kemper, “Quantitative Stain-free and Continuous Multimodal Monitoring of Wound Healing In Vitro with Digital Holographic Microscopy,” PLoS ONE 9(9), e107317 (2014).
[Crossref] [PubMed]

Bhaduri, B.

Bon, P.

Boss, D.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
[Crossref]

Bredebusch, I.

B. Kemper, S. Kosmeier, P. Langehanenberg, G. von Bally, I. Bredebusch, W. Domschke, and J. Schnekenburger, “Integral refractive index determination of living suspension cells by multi focus digital holographic phase contrast microscopy,” J. Biomed. Opt. 12(5), 054009 (2007).
[Crossref] [PubMed]

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, “Investigation of living pancreas tumor cells by digital holographic microscopy,” J. Biomed. Opt. 11(3), 034005 (2006).
[Crossref] [PubMed]

Brückner, M.

D. Bettenworth, P. Lenz, P. Krausewitz, M. Brückner, S. Ketelhut, D. Domagk, and B. Kemper, “Quantitative Stain-free and Continuous Multimodal Monitoring of Wound Healing In Vitro with Digital Holographic Microscopy,” PLoS ONE 9(9), e107317 (2014).
[Crossref] [PubMed]

Cano, E.

B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
[Crossref] [PubMed]

Carl, D.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, “Investigation of living pancreas tumor cells by digital holographic microscopy,” J. Biomed. Opt. 11(3), 034005 (2006).
[Crossref] [PubMed]

D. Carl, B. Kemper, G. Wernicke, and G. von Bally, “Parameter-optimized digital holographic microscope for high-resolution living-cell analysis,” Appl. Opt. 43(36), 6536–6544 (2004).
[Crossref] [PubMed]

Carpenter, A. E.

A. E. Carpenter, T. R. Jones, M. R. Lamprecht, C. Clarke, I. H. Kang, O. Friman, D. A. Guertin, J. H. Chang, R. A. Lindquist, J. Moffat, P. Golland, and D. M. Sabatini, “CellProfiler: image analysis software for identifying and quantifying cell phenotypes,” Genome Biol. 7(10), R100 (2006).
[Crossref] [PubMed]

Chang, J. H.

A. E. Carpenter, T. R. Jones, M. R. Lamprecht, C. Clarke, I. H. Kang, O. Friman, D. A. Guertin, J. H. Chang, R. A. Lindquist, J. Moffat, P. Golland, and D. M. Sabatini, “CellProfiler: image analysis software for identifying and quantifying cell phenotypes,” Genome Biol. 7(10), R100 (2006).
[Crossref] [PubMed]

Choi, W.

Y. Choi, T. D. Yang, K. J. Lee, and W. Choi, “Full-field and single-shot quantitative phase microscopy using dynamic speckle illumination,” Opt. Lett. 36(13), 2465–2467 (2011).
[Crossref] [PubMed]

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods 4(9), 717–719 (2007).
[Crossref] [PubMed]

Choi, Y.

Clarke, C.

A. E. Carpenter, T. R. Jones, M. R. Lamprecht, C. Clarke, I. H. Kang, O. Friman, D. A. Guertin, J. H. Chang, R. A. Lindquist, J. Moffat, P. Golland, and D. M. Sabatini, “CellProfiler: image analysis software for identifying and quantifying cell phenotypes,” Genome Biol. 7(10), R100 (2006).
[Crossref] [PubMed]

Colicchio, B.

Colomb, T.

B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
[Crossref] [PubMed]

Cotte, Y.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
[Crossref]

Cuche, E.

Dasari, R. R.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods 4(9), 717–719 (2007).
[Crossref] [PubMed]

Debailleul, M.

Depeursinge, C.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
[Crossref]

P. Marquet, C. Depeursinge, and P. J. Magistretti, “Exploring Neural Cell Dynamics with Digital Holographic Microscopy,” Annu. Rev. Biomed. Eng. 15(1), 407–431 (2013).
[Crossref] [PubMed]

B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
[Crossref] [PubMed]

E. Cuche, P. Marquet, and C. Depeursinge, “Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms,” Appl. Opt. 38(34), 6994–7001 (1999).
[Crossref] [PubMed]

Ding, H.

Dirksen, D.

B. Kemper, J. Kandulla, D. Dirksen, and G. von Bally, “Optimization of spatial phase shifting in endoscopic electronic-speckle-pattern-interferometry,” Opt. Commun. 217(1-6), 151–160 (2003).
[Crossref]

Domagk, D.

D. Bettenworth, P. Lenz, P. Krausewitz, M. Brückner, S. Ketelhut, D. Domagk, and B. Kemper, “Quantitative Stain-free and Continuous Multimodal Monitoring of Wound Healing In Vitro with Digital Holographic Microscopy,” PLoS ONE 9(9), e107317 (2014).
[Crossref] [PubMed]

Domschke, W.

B. Kemper, S. Kosmeier, P. Langehanenberg, G. von Bally, I. Bredebusch, W. Domschke, and J. Schnekenburger, “Integral refractive index determination of living suspension cells by multi focus digital holographic phase contrast microscopy,” J. Biomed. Opt. 12(5), 054009 (2007).
[Crossref] [PubMed]

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, “Investigation of living pancreas tumor cells by digital holographic microscopy,” J. Biomed. Opt. 11(3), 034005 (2006).
[Crossref] [PubMed]

Dubois, F.

Edwards, C.

Fang-Yen, C.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods 4(9), 717–719 (2007).
[Crossref] [PubMed]

Faridian, A.

Feld, M. S.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods 4(9), 717–719 (2007).
[Crossref] [PubMed]

Ferraro, P.

Finizio, A.

Friman, O.

A. E. Carpenter, T. R. Jones, M. R. Lamprecht, C. Clarke, I. H. Kang, O. Friman, D. A. Guertin, J. H. Chang, R. A. Lindquist, J. Moffat, P. Golland, and D. M. Sabatini, “CellProfiler: image analysis software for identifying and quantifying cell phenotypes,” Genome Biol. 7(10), R100 (2006).
[Crossref] [PubMed]

Galli, A.

Ganti, R.

Gao, P.

Georges, V.

Gillette, M. U.

Girshovitz, P.

Goddard, L. L.

Golland, P.

A. E. Carpenter, T. R. Jones, M. R. Lamprecht, C. Clarke, I. H. Kang, O. Friman, D. A. Guertin, J. H. Chang, R. A. Lindquist, J. Moffat, P. Golland, and D. M. Sabatini, “CellProfiler: image analysis software for identifying and quantifying cell phenotypes,” Genome Biol. 7(10), R100 (2006).
[Crossref] [PubMed]

Guertin, D. A.

A. E. Carpenter, T. R. Jones, M. R. Lamprecht, C. Clarke, I. H. Kang, O. Friman, D. A. Guertin, J. H. Chang, R. A. Lindquist, J. Moffat, P. Golland, and D. M. Sabatini, “CellProfiler: image analysis software for identifying and quantifying cell phenotypes,” Genome Biol. 7(10), R100 (2006).
[Crossref] [PubMed]

Haeberlé, O.

Hwang, S. W.

Joannes, L.

Jones, T. R.

A. E. Carpenter, T. R. Jones, M. R. Lamprecht, C. Clarke, I. H. Kang, O. Friman, D. A. Guertin, J. H. Chang, R. A. Lindquist, J. Moffat, P. Golland, and D. M. Sabatini, “CellProfiler: image analysis software for identifying and quantifying cell phenotypes,” Genome Biol. 7(10), R100 (2006).
[Crossref] [PubMed]

Jourdain, P.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
[Crossref]

Kandulla, J.

B. Kemper, J. Kandulla, D. Dirksen, and G. von Bally, “Optimization of spatial phase shifting in endoscopic electronic-speckle-pattern-interferometry,” Opt. Commun. 217(1-6), 151–160 (2003).
[Crossref]

Kang, I. H.

A. E. Carpenter, T. R. Jones, M. R. Lamprecht, C. Clarke, I. H. Kang, O. Friman, D. A. Guertin, J. H. Chang, R. A. Lindquist, J. Moffat, P. Golland, and D. M. Sabatini, “CellProfiler: image analysis software for identifying and quantifying cell phenotypes,” Genome Biol. 7(10), R100 (2006).
[Crossref] [PubMed]

Kang, X.

Kemper, B.

D. Bettenworth, P. Lenz, P. Krausewitz, M. Brückner, S. Ketelhut, D. Domagk, and B. Kemper, “Quantitative Stain-free and Continuous Multimodal Monitoring of Wound Healing In Vitro with Digital Holographic Microscopy,” PLoS ONE 9(9), e107317 (2014).
[Crossref] [PubMed]

S. Kosmeier, P. Langehanenberg, G. von Bally, and B. Kemper, “Reduction of parasitic interferences in digital holographic microscopy by numerically decreased coherence length,” Appl. Phys. B 106(1), 107–115 (2012).
[Crossref]

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, “Simplified approach for quantitative digital holographic phase contrast imaging of living cells,” J. Biomed. Opt. 16(2), 026014 (2011).
[Crossref] [PubMed]

B. Kemper, S. Kosmeier, P. Langehanenberg, S. Przibilla, C. Remmersmann, S. Stürwald, and G. von Bally, “Application of 3D tracking, LED illumination and multi-wavelength techniques for quantitative cell analysis in digital holographic microscopy,” Proc. SPIE 7184, 71840R (2009).
[Crossref]

B. Kemper and G. von Bally, “Digital holographic microscopy for live cell applications and technical inspection,” Appl. Opt. 47(4), A52–A61 (2008).
[Crossref] [PubMed]

B. Kemper, S. Stürwald, C. Remmersmann, P. Langehanenberg, and G. von Bally, “Characterisation of light emitting diodes (LEDs) for application in digital holographic microscopy for inspection of micro and nanostructured surfaces,” Opt. Lasers Eng. 46(7), 499–507 (2008).
[Crossref]

B. Kemper, S. Kosmeier, P. Langehanenberg, G. von Bally, I. Bredebusch, W. Domschke, and J. Schnekenburger, “Integral refractive index determination of living suspension cells by multi focus digital holographic phase contrast microscopy,” J. Biomed. Opt. 12(5), 054009 (2007).
[Crossref] [PubMed]

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, “Investigation of living pancreas tumor cells by digital holographic microscopy,” J. Biomed. Opt. 11(3), 034005 (2006).
[Crossref] [PubMed]

D. Carl, B. Kemper, G. Wernicke, and G. von Bally, “Parameter-optimized digital holographic microscope for high-resolution living-cell analysis,” Appl. Opt. 43(36), 6536–6544 (2004).
[Crossref] [PubMed]

B. Kemper, J. Kandulla, D. Dirksen, and G. von Bally, “Optimization of spatial phase shifting in endoscopic electronic-speckle-pattern-interferometry,” Opt. Commun. 217(1-6), 151–160 (2003).
[Crossref]

Ketelhut, S.

D. Bettenworth, P. Lenz, P. Krausewitz, M. Brückner, S. Ketelhut, D. Domagk, and B. Kemper, “Quantitative Stain-free and Continuous Multimodal Monitoring of Wound Healing In Vitro with Digital Holographic Microscopy,” PLoS ONE 9(9), e107317 (2014).
[Crossref] [PubMed]

Kosmeier, S.

S. Kosmeier, P. Langehanenberg, G. von Bally, and B. Kemper, “Reduction of parasitic interferences in digital holographic microscopy by numerically decreased coherence length,” Appl. Phys. B 106(1), 107–115 (2012).
[Crossref]

B. Kemper, S. Kosmeier, P. Langehanenberg, S. Przibilla, C. Remmersmann, S. Stürwald, and G. von Bally, “Application of 3D tracking, LED illumination and multi-wavelength techniques for quantitative cell analysis in digital holographic microscopy,” Proc. SPIE 7184, 71840R (2009).
[Crossref]

B. Kemper, S. Kosmeier, P. Langehanenberg, G. von Bally, I. Bredebusch, W. Domschke, and J. Schnekenburger, “Integral refractive index determination of living suspension cells by multi focus digital holographic phase contrast microscopy,” J. Biomed. Opt. 12(5), 054009 (2007).
[Crossref] [PubMed]

Krausewitz, P.

D. Bettenworth, P. Lenz, P. Krausewitz, M. Brückner, S. Ketelhut, D. Domagk, and B. Kemper, “Quantitative Stain-free and Continuous Multimodal Monitoring of Wound Healing In Vitro with Digital Holographic Microscopy,” PLoS ONE 9(9), e107317 (2014).
[Crossref] [PubMed]

Kühn, J.

B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
[Crossref] [PubMed]

Lamprecht, M. R.

A. E. Carpenter, T. R. Jones, M. R. Lamprecht, C. Clarke, I. H. Kang, O. Friman, D. A. Guertin, J. H. Chang, R. A. Lindquist, J. Moffat, P. Golland, and D. M. Sabatini, “CellProfiler: image analysis software for identifying and quantifying cell phenotypes,” Genome Biol. 7(10), R100 (2006).
[Crossref] [PubMed]

Langehanenberg, P.

S. Kosmeier, P. Langehanenberg, G. von Bally, and B. Kemper, “Reduction of parasitic interferences in digital holographic microscopy by numerically decreased coherence length,” Appl. Phys. B 106(1), 107–115 (2012).
[Crossref]

B. Kemper, S. Kosmeier, P. Langehanenberg, S. Przibilla, C. Remmersmann, S. Stürwald, and G. von Bally, “Application of 3D tracking, LED illumination and multi-wavelength techniques for quantitative cell analysis in digital holographic microscopy,” Proc. SPIE 7184, 71840R (2009).
[Crossref]

B. Kemper, S. Stürwald, C. Remmersmann, P. Langehanenberg, and G. von Bally, “Characterisation of light emitting diodes (LEDs) for application in digital holographic microscopy for inspection of micro and nanostructured surfaces,” Opt. Lasers Eng. 46(7), 499–507 (2008).
[Crossref]

B. Kemper, S. Kosmeier, P. Langehanenberg, G. von Bally, I. Bredebusch, W. Domschke, and J. Schnekenburger, “Integral refractive index determination of living suspension cells by multi focus digital holographic phase contrast microscopy,” J. Biomed. Opt. 12(5), 054009 (2007).
[Crossref] [PubMed]

Lee, K. J.

Legros, J. C.

Lenz, P.

D. Bettenworth, P. Lenz, P. Krausewitz, M. Brückner, S. Ketelhut, D. Domagk, and B. Kemper, “Quantitative Stain-free and Continuous Multimodal Monitoring of Wound Healing In Vitro with Digital Holographic Microscopy,” PLoS ONE 9(9), e107317 (2014).
[Crossref] [PubMed]

Li, R.

Lindquist, R. A.

A. E. Carpenter, T. R. Jones, M. R. Lamprecht, C. Clarke, I. H. Kang, O. Friman, D. A. Guertin, J. H. Chang, R. A. Lindquist, J. Moffat, P. Golland, and D. M. Sabatini, “CellProfiler: image analysis software for identifying and quantifying cell phenotypes,” Genome Biol. 7(10), R100 (2006).
[Crossref] [PubMed]

Liu, H.

Liu, S.

Lue, N.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods 4(9), 717–719 (2007).
[Crossref] [PubMed]

Magistretti, P.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
[Crossref]

Magistretti, P. J.

P. Marquet, C. Depeursinge, and P. J. Magistretti, “Exploring Neural Cell Dynamics with Digital Holographic Microscopy,” Annu. Rev. Biomed. Eng. 15(1), 407–431 (2013).
[Crossref] [PubMed]

B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
[Crossref] [PubMed]

Marquet, P.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
[Crossref]

P. Marquet, C. Depeursinge, and P. J. Magistretti, “Exploring Neural Cell Dynamics with Digital Holographic Microscopy,” Annu. Rev. Biomed. Eng. 15(1), 407–431 (2013).
[Crossref] [PubMed]

B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
[Crossref] [PubMed]

E. Cuche, P. Marquet, and C. Depeursinge, “Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms,” Appl. Opt. 38(34), 6994–7001 (1999).
[Crossref] [PubMed]

Maucort, G.

McKeown, S. J.

Miccio, L.

Millet, L.

Mir, M.

Moffat, J.

A. E. Carpenter, T. R. Jones, M. R. Lamprecht, C. Clarke, I. H. Kang, O. Friman, D. A. Guertin, J. H. Chang, R. A. Lindquist, J. Moffat, P. Golland, and D. M. Sabatini, “CellProfiler: image analysis software for identifying and quantifying cell phenotypes,” Genome Biol. 7(10), R100 (2006).
[Crossref] [PubMed]

Monneret, S.

Morin, R.

Oh, S.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods 4(9), 717–719 (2007).
[Crossref] [PubMed]

Osten, W.

Pan, F.

Pavillon, N.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
[Crossref]

Pedrini, G.

Pham, H.

Popescu, G.

Przibilla, S.

B. Kemper, S. Kosmeier, P. Langehanenberg, S. Przibilla, C. Remmersmann, S. Stürwald, and G. von Bally, “Application of 3D tracking, LED illumination and multi-wavelength techniques for quantitative cell analysis in digital holographic microscopy,” Proc. SPIE 7184, 71840R (2009).
[Crossref]

Puglisi, R.

Rappaz, B.

B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
[Crossref] [PubMed]

Remmersmann, C.

B. Kemper, S. Kosmeier, P. Langehanenberg, S. Przibilla, C. Remmersmann, S. Stürwald, and G. von Bally, “Application of 3D tracking, LED illumination and multi-wavelength techniques for quantitative cell analysis in digital holographic microscopy,” Proc. SPIE 7184, 71840R (2009).
[Crossref]

B. Kemper, S. Stürwald, C. Remmersmann, P. Langehanenberg, and G. von Bally, “Characterisation of light emitting diodes (LEDs) for application in digital holographic microscopy for inspection of micro and nanostructured surfaces,” Opt. Lasers Eng. 46(7), 499–507 (2008).
[Crossref]

Rogers, J.

Rogers, J. A.

Rommel, C. E.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, “Simplified approach for quantitative digital holographic phase contrast imaging of living cells,” J. Biomed. Opt. 16(2), 026014 (2011).
[Crossref] [PubMed]

Rong, L.

Sabatini, D. M.

A. E. Carpenter, T. R. Jones, M. R. Lamprecht, C. Clarke, I. H. Kang, O. Friman, D. A. Guertin, J. H. Chang, R. A. Lindquist, J. Moffat, P. Golland, and D. M. Sabatini, “CellProfiler: image analysis software for identifying and quantifying cell phenotypes,” Genome Biol. 7(10), R100 (2006).
[Crossref] [PubMed]

Schäfer, M.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, “Investigation of living pancreas tumor cells by digital holographic microscopy,” J. Biomed. Opt. 11(3), 034005 (2006).
[Crossref] [PubMed]

Schnekenburger, J.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, “Simplified approach for quantitative digital holographic phase contrast imaging of living cells,” J. Biomed. Opt. 16(2), 026014 (2011).
[Crossref] [PubMed]

B. Kemper, S. Kosmeier, P. Langehanenberg, G. von Bally, I. Bredebusch, W. Domschke, and J. Schnekenburger, “Integral refractive index determination of living suspension cells by multi focus digital holographic phase contrast microscopy,” J. Biomed. Opt. 12(5), 054009 (2007).
[Crossref] [PubMed]

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, “Investigation of living pancreas tumor cells by digital holographic microscopy,” J. Biomed. Opt. 11(3), 034005 (2006).
[Crossref] [PubMed]

Shaked, N. T.

Simanis, V.

B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
[Crossref] [PubMed]

Simon, B.

Singh, A. K.

Stürwald, S.

B. Kemper, S. Kosmeier, P. Langehanenberg, S. Przibilla, C. Remmersmann, S. Stürwald, and G. von Bally, “Application of 3D tracking, LED illumination and multi-wavelength techniques for quantitative cell analysis in digital holographic microscopy,” Proc. SPIE 7184, 71840R (2009).
[Crossref]

B. Kemper, S. Stürwald, C. Remmersmann, P. Langehanenberg, and G. von Bally, “Characterisation of light emitting diodes (LEDs) for application in digital holographic microscopy for inspection of micro and nanostructured surfaces,” Opt. Lasers Eng. 46(7), 499–507 (2008).
[Crossref]

Tangella, K.

Toy, F.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
[Crossref]

Unarunotai, S.

Vollmer, A.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, “Simplified approach for quantitative digital holographic phase contrast imaging of living cells,” J. Biomed. Opt. 16(2), 026014 (2011).
[Crossref] [PubMed]

von Bally, G.

S. Kosmeier, P. Langehanenberg, G. von Bally, and B. Kemper, “Reduction of parasitic interferences in digital holographic microscopy by numerically decreased coherence length,” Appl. Phys. B 106(1), 107–115 (2012).
[Crossref]

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, “Simplified approach for quantitative digital holographic phase contrast imaging of living cells,” J. Biomed. Opt. 16(2), 026014 (2011).
[Crossref] [PubMed]

B. Kemper, S. Kosmeier, P. Langehanenberg, S. Przibilla, C. Remmersmann, S. Stürwald, and G. von Bally, “Application of 3D tracking, LED illumination and multi-wavelength techniques for quantitative cell analysis in digital holographic microscopy,” Proc. SPIE 7184, 71840R (2009).
[Crossref]

B. Kemper and G. von Bally, “Digital holographic microscopy for live cell applications and technical inspection,” Appl. Opt. 47(4), A52–A61 (2008).
[Crossref] [PubMed]

B. Kemper, S. Stürwald, C. Remmersmann, P. Langehanenberg, and G. von Bally, “Characterisation of light emitting diodes (LEDs) for application in digital holographic microscopy for inspection of micro and nanostructured surfaces,” Opt. Lasers Eng. 46(7), 499–507 (2008).
[Crossref]

B. Kemper, S. Kosmeier, P. Langehanenberg, G. von Bally, I. Bredebusch, W. Domschke, and J. Schnekenburger, “Integral refractive index determination of living suspension cells by multi focus digital holographic phase contrast microscopy,” J. Biomed. Opt. 12(5), 054009 (2007).
[Crossref] [PubMed]

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, “Investigation of living pancreas tumor cells by digital holographic microscopy,” J. Biomed. Opt. 11(3), 034005 (2006).
[Crossref] [PubMed]

D. Carl, B. Kemper, G. Wernicke, and G. von Bally, “Parameter-optimized digital holographic microscope for high-resolution living-cell analysis,” Appl. Opt. 43(36), 6536–6544 (2004).
[Crossref] [PubMed]

B. Kemper, J. Kandulla, D. Dirksen, and G. von Bally, “Optimization of spatial phase shifting in endoscopic electronic-speckle-pattern-interferometry,” Opt. Commun. 217(1-6), 151–160 (2003).
[Crossref]

Wang, K.

Wang, Z.

Wattellier, B.

Wernicke, G.

Xiao, W.

Yang, T. D.

Yodh, A. G.

Yunker, P. J.

Zhou, R.

Annu. Rev. Biomed. Eng. (1)

P. Marquet, C. Depeursinge, and P. J. Magistretti, “Exploring Neural Cell Dynamics with Digital Holographic Microscopy,” Annu. Rev. Biomed. Eng. 15(1), 407–431 (2013).
[Crossref] [PubMed]

Appl. Opt. (6)

Appl. Phys. B (1)

S. Kosmeier, P. Langehanenberg, G. von Bally, and B. Kemper, “Reduction of parasitic interferences in digital holographic microscopy by numerically decreased coherence length,” Appl. Phys. B 106(1), 107–115 (2012).
[Crossref]

Biomed. Opt. Express (2)

Chin. Opt. Lett. (2)

Genome Biol. (1)

A. E. Carpenter, T. R. Jones, M. R. Lamprecht, C. Clarke, I. H. Kang, O. Friman, D. A. Guertin, J. H. Chang, R. A. Lindquist, J. Moffat, P. Golland, and D. M. Sabatini, “CellProfiler: image analysis software for identifying and quantifying cell phenotypes,” Genome Biol. 7(10), R100 (2006).
[Crossref] [PubMed]

J. Biomed. Opt. (4)

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, “Simplified approach for quantitative digital holographic phase contrast imaging of living cells,” J. Biomed. Opt. 16(2), 026014 (2011).
[Crossref] [PubMed]

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, “Investigation of living pancreas tumor cells by digital holographic microscopy,” J. Biomed. Opt. 11(3), 034005 (2006).
[Crossref] [PubMed]

B. Kemper, S. Kosmeier, P. Langehanenberg, G. von Bally, I. Bredebusch, W. Domschke, and J. Schnekenburger, “Integral refractive index determination of living suspension cells by multi focus digital holographic phase contrast microscopy,” J. Biomed. Opt. 12(5), 054009 (2007).
[Crossref] [PubMed]

B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
[Crossref] [PubMed]

Nat. Methods (1)

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods 4(9), 717–719 (2007).
[Crossref] [PubMed]

Nat. Photonics (1)

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
[Crossref]

Nature (1)

R. Barer, “Interference microscopy and mass determination,” Nature 169(4296), 366–367 (1952).
[Crossref] [PubMed]

Opt. Commun. (1)

B. Kemper, J. Kandulla, D. Dirksen, and G. von Bally, “Optimization of spatial phase shifting in endoscopic electronic-speckle-pattern-interferometry,” Opt. Commun. 217(1-6), 151–160 (2003).
[Crossref]

Opt. Express (5)

Opt. Lasers Eng. (1)

B. Kemper, S. Stürwald, C. Remmersmann, P. Langehanenberg, and G. von Bally, “Characterisation of light emitting diodes (LEDs) for application in digital holographic microscopy for inspection of micro and nanostructured surfaces,” Opt. Lasers Eng. 46(7), 499–507 (2008).
[Crossref]

Opt. Lett. (4)

PLoS ONE (1)

D. Bettenworth, P. Lenz, P. Krausewitz, M. Brückner, S. Ketelhut, D. Domagk, and B. Kemper, “Quantitative Stain-free and Continuous Multimodal Monitoring of Wound Healing In Vitro with Digital Holographic Microscopy,” PLoS ONE 9(9), e107317 (2014).
[Crossref] [PubMed]

Proc. SPIE (1)

B. Kemper, S. Kosmeier, P. Langehanenberg, S. Przibilla, C. Remmersmann, S. Stürwald, and G. von Bally, “Application of 3D tracking, LED illumination and multi-wavelength techniques for quantitative cell analysis in digital holographic microscopy,” Proc. SPIE 7184, 71840R (2009).
[Crossref]

Other (4)

J. C. Dainty, Laser Speckle and Related Phenomena (Springer, 1984).

G. Popescu, Quantitative phase imaging of cells and tissues (McGraw Hill, 2011).

N. Shaked, Z. Zalevsky, and L. L. Satterwhite, eds., Biomedical Optical Phase Microscopy and Nanoscopy (Elsevier, 2012).

P. Langehanenberg, G. von Bally, and B. Kemper, “Autofocussing in Digital Holographic Microscopy,” 3D Res. 2, 01004 (2011).

Supplementary Material (4)

» Media 1: MP4 (1959 KB)     
» Media 2: MP4 (5084 KB)     
» Media 3: MP4 (1959 KB)     
» Media 4: MP4 (5084 KB)     

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Figures (5)

Fig. 1
Fig. 1 Self-interference DHM setup with modulated object illumination by an electrically focus tunable lens. SM, single mode fiber, delivering light from a frequency doubled Nd:YAG laser (λ = 532 nm); FL, focus tunable lens; BS1/BS2, non-polarizing beam splitters; CL, condenser lens; S, sample; OL, objective lens; TL, tube lens; RL, relay lens; M1/M2, mirrors.
Fig. 2
Fig. 2 Amplitude images of an USAF 1951 test target coded to 256 gray levels, acquired with a 10x microscope lens (NA = 0.3). (a) Amplitude from a single hologram. (b) averaged amplitude reconstructed from a sequence of N = 15 holograms acquired under illumination modulation. (c), (d) magnified areas of the smallest test chart structures. (e) cross-sections through the magnified areas along the white lines in (c) and (d). The operation principle of the method, the underlying series of off-axis holograms and the corresponding series of single reconstructed amplitude images are illustrated in Media 1.
Fig. 3
Fig. 3 (a), (c), (e) Signal to noise ratio (SNR) of the USAF test target structures in amplitude images for microscope lenses with different magnifications (10x: Zeiss 10x/ EC Plan-Neofluar, 40x: Zeiss 40x/0.6 Korr LD Plan-Neofluar, 63x: Zeiss 63x/0.75 Korr LD Plan-Neofluar); (b), (d), (f) phase noise σ and related OPL variations in the corresponding quantitative phase images; N: number of off-axis holograms that were recorded during modulated illumination with the FL. Data points and error bars represent the mean values for SNR and σ that were acquired in ten different areas of the averaged amplitude and phase images as well as the corresponding standard deviations. The solid curves in (b), (d), (f) present the theoretically expected phase noise decrease.
Fig. 4
Fig. 4 (a) Representative segmented quantitative phase images of reconstructed holograms of human fibrosarcoma cells (HT-1080), acquired at different time points during a time-lapse experiment (Δt = 5 min, tmax = 11.5 h). Left column: phase images reconstructed from single holograms (N = 1). Right column: Averaged phase images retrieved from N = 15 holograms that were acquired during modulated illumination with the FL. Arrows mark differences of the detected cell covered surface. Cross-sections through a cell along the dashed lines are plotted in Fig. 5(a). (b) Corresponding numerically simulated differential interference contrast images calculated by the first derivative of the images in (a) in x-direction (Media 2).
Fig. 5
Fig. 5 (a) Illustration of spatial phase noise reduction by cross-sections through single (N = 1) and averaged (N = 15) quantitative phase distributions Δφ of a HT-1080 cell at t = 11.3 h (see dashed lines in in Fig. 4(a)). (b)-(d) Temporal development of cell growth parameters calculated from the segmented phase images in Fig. 4. (b) Area Sc covered by the cells, (c) Average phase contrast Δ φ ¯ in the area Sc and the corresponding average cell thickness d ¯ . (d) Cellular dry mass DM in the area Sc.

Metrics