Abstract

A fluorescence lifetime imaging microscopy (FLIM) integrated with two-photon excitation technique was developed. A wavelength-tunable femtosecond pulsed laser with nominal pulse repetition rate of 76-MHz was used to acquire FLIM images with a high pixel rate of 3.91 MHz by processing the pulsed two-photon fluorescence signal. Analog mean-delay (AMD) method was adopted to accelerate the lifetime measurement process and to visualize lifetime map in real-time. As a result, rapid tomographic visualization of both structural and chemical properties of the tissues was possible with longer depth penetration and lower photo-damage compared to the conventional single-photon FLIM techniques.

© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Full Article  |  PDF Article
OSA Recommended Articles
GPU accelerated real-time confocal fluorescence lifetime imaging microscopy (FLIM) based on the analog mean-delay (AMD) method

Byungyeon Kim, Byungjun Park, Seungrag Lee, and Youngjae Won
Biomed. Opt. Express 7(12) 5055-5065 (2016)

High-speed time-resolved laser-scanning microscopy using the line-to-pixel referencing method

Jiheun Ryu, Jayul Kim, Hyunjun Kim, Jae-heon Jeong, Hak-jun Lee, Hongki Yoo, and Dae-Gab Gweon
Appl. Opt. 55(32) 9033-9041 (2016)

Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate

Matthias Eibl, Sebastian Karpf, Daniel Weng, Hubertus Hakert, Tom Pfeiffer, Jan Philip Kolb, and Robert Huber
Biomed. Opt. Express 8(7) 3132-3142 (2017)

References

  • View by:
  • |
  • |
  • |

  1. N. Barkai and S. Leibler, “Robustness in simple biochemical networks,” Nature 387(6636), 913–917 (1997).
    [Crossref] [PubMed]
  2. M. Y. Berezin and S. Achilefu, “Fluorescence Lifetime Measurements and Biological Imaging,” Chem. Rev. 110(5), 2641–2684 (2010).
    [Crossref] [PubMed]
  3. W. Becker, “Fluorescence lifetime imaging--techniques and applications,” J. Microsc. 247(2), 119–136 (2012).
    [Crossref] [PubMed]
  4. N. P. Galletly, J. McGinty, C. Dunsby, F. Teixeira, J. Requejo-Isidro, I. Munro, D. S. Elson, M. A. Neil, A. C. Chu, P. M. French, and G. W. Stamp, “Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin,” Br. J. Dermatol. 159(1), 152–161 (2008).
    [Crossref] [PubMed]
  5. M. S. Islam, M. Honma, T. Nakabayashi, M. Kinjo, and N. Ohta, “pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells,” Int. J. Mol. Sci. 14(1), 1952–1963 (2013).
    [Crossref] [PubMed]
  6. J. M. Jabbour, S. Cheng, B. H. Malik, R. Cuenca, J. A. Jo, J. Wright, Y. S. L. Cheng, and K. C. Maitland, “Fluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer,” J. Biomed. Opt. 18(4), 046012 (2013).
    [Crossref] [PubMed]
  7. J. Park, P. Pande, S. Shrestha, F. Clubb, B. E. Applegate, and J. A. Jo, “Biochemical characterization of atherosclerotic plaques by endogenous multispectral fluorescence lifetime imaging microscopy,” Atherosclerosis 220(2), 394–401 (2012).
    [Crossref] [PubMed]
  8. P. I. H. Bastiaens and A. Squire, “Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell,” Trends Cell Biol. 9(2), 48–52 (1999).
    [Crossref] [PubMed]
  9. D. Bray, “Protein Molecules as Computational Elements in Living Cells,” Nature 376(6538), 307–312 (1995).
    [Crossref] [PubMed]
  10. S. Cheng, J. J. Rico-Jimenez, J. Jabbour, B. Malik, K. C. Maitland, J. Wright, Y. S. L. Cheng, and J. A. Jo, “Flexible endoscope for continuous in vivo multispectral fluorescence lifetime imaging,” Opt. Lett. 38(9), 1515–1517 (2013).
    [Crossref] [PubMed]
  11. T. Hinsdale, C. Olsovsky, J. J. Rico-Jimenez, K. C. Maitland, J. A. Jo, and B. H. Malik, “Optically sectioned wide-field fluorescence lifetime imaging microscopy enabled by structured illumination,” Biomed. Opt. Express 8(3), 1455–1465 (2017).
    [Crossref] [PubMed]
  12. J. L. Rinnenthal, C. Börnchen, H. Radbruch, V. Andresen, A. Mossakowski, V. Siffrin, T. Seelemann, H. Spiecker, I. Moll, J. Herz, A. E. Hauser, F. Zipp, M. J. Behne, and R. Niesner, “Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation,” PLoS One 8(4), e60100 (2013).
    [Crossref] [PubMed]
  13. W. Becker, Advanced Time-Correlated Single Photon Counting Applications (Springer International Publishing, 2015).
  14. S. Cheng, R. M. Cuenca, B. Liu, B. H. Malik, J. M. Jabbour, K. C. Maitland, J. Wright, Y. S. L. Cheng, and J. A. Jo, “Handheld multispectral fluorescence lifetime imaging system for in vivo applications,” Biomed. Opt. Express 5(3), 921–931 (2014).
    [Crossref] [PubMed]
  15. B. H. Malik, J. Lee, S. Cheng, R. Cuenca, J. M. Jabbour, Y. S. L. Cheng, J. M. Wright, B. Ahmed, K. C. Maitland, and J. A. Jo, “Objective Detection of Oral Carcinoma with Multispectral Fluorescence Lifetime Imaging In Vivo,” Photochem. Photobiol. 92(5), 694–701 (2016).
    [Crossref] [PubMed]
  16. X. Y. Dow, S. Z. Sullivan, R. D. Muir, and G. J. Simpson, “Video-rate two-photon excited fluorescence lifetime imaging system with interleaved digitization,” Opt. Lett. 40(14), 3296–3299 (2015).
    [Crossref] [PubMed]
  17. M. G. Giacomelli, Y. Sheikine, H. Vardeh, J. L. Connolly, and J. G. Fujimoto, “Rapid imaging of surgical breast excisions using direct temporal sampling two photon fluorescent lifetime imaging,” Biomed. Opt. Express 6(11), 4317–4325 (2015).
    [Crossref] [PubMed]
  18. S. Moon, Y. Won, and D. Y. Kim, “Analog mean-delay method for high-speed fluorescence lifetime measurement,” Opt. Express 17(4), 2834–2849 (2009).
    [Crossref] [PubMed]
  19. Y. J. Won, S. Moon, W. T. Han, and D. Y. Kim, “Referencing techniques for the analog mean-delay method in fluorescence lifetime imaging,” J. Opt. Soc. Am. A 27(11), 2402–2410 (2010).
    [Crossref] [PubMed]
  20. Y. J. Won, W. T. Han, and D. Y. Kim, “Precision and accuracy of the analog mean-delay method for high-speed fluorescence lifetime measurement,” J. Opt. Soc. Am. A 28(10), 2026–2032 (2011).
    [Crossref] [PubMed]
  21. Y. Won, S. Moon, W. Yang, D. Kim, W. T. Han, and D. Y. Kim, “High-speed confocal fluorescence lifetime imaging microscopy (FLIM) with the analog mean delay (AMD) method,” Opt. Express 19(4), 3396–3405 (2011).
    [Crossref] [PubMed]
  22. B. Kim, B. Park, S. Lee, and Y. Won, “GPU accelerated real-time confocal fluorescence lifetime imaging microscopy (FLIM) based on the analog mean-delay (AMD) method,” Biomed. Opt. Express 7(12), 5055–5065 (2016).
    [Crossref] [PubMed]
  23. J. Kim, J. Ryu, and D. Gweon, “Real-time Fluorescence Lifetime Imaging Microscopy Implementation by Analog Mean-Delay Method through Parallel Data Processing,” Applied Microscopy 46(1), 6–13 (2016).
    [Crossref]
  24. J. Ryu, J. Kim, H. Kim, J. H. Jeong, H. J. Lee, H. Yoo, and D. G. Gweon, “High-speed time-resolved laser-scanning microscopy using the line-to-pixel referencing method,” Appl. Opt. 55(32), 9033–9041 (2016).
    [Crossref] [PubMed]
  25. H. S. Nam, W. J. Kang, M. W. Lee, J. W. Song, J. W. Kim, W. Y. Oh, and H. Yoo, “Multispectral analog-mean-delay fluorescence lifetime imaging combined with optical coherence tomography,” Biomed. Opt. Express 9(4), 1930–1947 (2018).
    [Crossref] [PubMed]
  26. Y. Dancik, A. Favre, C. J. Loy, A. V. Zvyagin, and M. S. Roberts, “Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentation in vivo,” J. Biomed. Opt. 18(2), 026022 (2013).
    [Crossref] [PubMed]
  27. Laser Institute of America, “National Standard for Safe Use of Lasers ANSI Z136.1-2000,” (American National Standards Institute, Inc., 2000).
  28. P. Kapusta, R. Erdmann, U. Ortmann, and M. Wahl, “Time-resolved fluorescence anisotropy measurements made simple,” J. Fluoresc. 13(2), 179–183 (2003).
    [Crossref]
  29. A. S. Kristoffersen, S. R. Erga, B. Hamre, and Ø. Frette, “Testing Fluorescence Lifetime Standards using Two-Photon Excitation and Time-Domain Instrumentation: Rhodamine B, Coumarin 6 and Lucifer Yellow,” J. Fluoresc. 24(4), 1015–1024 (2014).
    [Crossref] [PubMed]
  30. C. Li, C. Pitsillides, J. M. Runnels, D. Côté, and C. P. Lin, “Multiphoton Microscopy of Live Tissues With Ultraviolet Autofluorescence,” IEEE J. Sel. Top. Quantum Electron 16(3), 516–523 (2010).
    [Crossref]
  31. J. R. Lakowicz, H. Szmacinski, K. Nowaczyk, and M. L. Johnson, “Fluorescence lifetime imaging of free and protein-bound NADH,” Proc. Natl. Acad. Sci. U.S.A. 89(4), 1271–1275 (1992).
    [Crossref] [PubMed]
  32. T. Hato, S. Winfree, R. Day, R. M. Sandoval, B. A. Molitoris, M. C. Yoder, R. C. Wiggins, Y. Zheng, K. W. Dunn, and P. C. Dagher, “Two-Photon Intravital Fluorescence Lifetime Imaging of the Kidney Reveals Cell-Type Specific Metabolic Signatures,” J. Am. Soc. Nephrol. 28(8), 2420–2430 (2017).
    [Crossref] [PubMed]
  33. A. F. Tarantal, H. Chen, T. T. Shi, C. H. Lu, A. B. Fang, S. Buckley, M. Kolb, J. Gauldie, D. Warburton, and W. Shi, “Overexpression of transforming growth factor-beta1 in fetal monkey lung results in prenatal pulmonary fibrosis,” Eur. Respir. J. 36(4), 907–914 (2010).
    [Crossref] [PubMed]
  34. I. Pavlova, K. R. Hume, S. A. Yazinski, J. Flanders, T. L. Southard, R. S. Weiss, and W. W. Webb, “Multiphoton microscopy and microspectroscopy for diagnostics of inflammatory and neoplastic lung,” J. Biomed. Opt. 17(3), 036014 (2012).
    [Crossref] [PubMed]
  35. H. Fehrenbach, “Alveolar epithelial type II cell: defender of the alveolus revisited,” Respir. Res. 2(1), 33–46 (2001).
    [Crossref] [PubMed]

2018 (1)

2017 (2)

T. Hinsdale, C. Olsovsky, J. J. Rico-Jimenez, K. C. Maitland, J. A. Jo, and B. H. Malik, “Optically sectioned wide-field fluorescence lifetime imaging microscopy enabled by structured illumination,” Biomed. Opt. Express 8(3), 1455–1465 (2017).
[Crossref] [PubMed]

T. Hato, S. Winfree, R. Day, R. M. Sandoval, B. A. Molitoris, M. C. Yoder, R. C. Wiggins, Y. Zheng, K. W. Dunn, and P. C. Dagher, “Two-Photon Intravital Fluorescence Lifetime Imaging of the Kidney Reveals Cell-Type Specific Metabolic Signatures,” J. Am. Soc. Nephrol. 28(8), 2420–2430 (2017).
[Crossref] [PubMed]

2016 (4)

B. H. Malik, J. Lee, S. Cheng, R. Cuenca, J. M. Jabbour, Y. S. L. Cheng, J. M. Wright, B. Ahmed, K. C. Maitland, and J. A. Jo, “Objective Detection of Oral Carcinoma with Multispectral Fluorescence Lifetime Imaging In Vivo,” Photochem. Photobiol. 92(5), 694–701 (2016).
[Crossref] [PubMed]

J. Ryu, J. Kim, H. Kim, J. H. Jeong, H. J. Lee, H. Yoo, and D. G. Gweon, “High-speed time-resolved laser-scanning microscopy using the line-to-pixel referencing method,” Appl. Opt. 55(32), 9033–9041 (2016).
[Crossref] [PubMed]

B. Kim, B. Park, S. Lee, and Y. Won, “GPU accelerated real-time confocal fluorescence lifetime imaging microscopy (FLIM) based on the analog mean-delay (AMD) method,” Biomed. Opt. Express 7(12), 5055–5065 (2016).
[Crossref] [PubMed]

J. Kim, J. Ryu, and D. Gweon, “Real-time Fluorescence Lifetime Imaging Microscopy Implementation by Analog Mean-Delay Method through Parallel Data Processing,” Applied Microscopy 46(1), 6–13 (2016).
[Crossref]

2015 (2)

2014 (2)

S. Cheng, R. M. Cuenca, B. Liu, B. H. Malik, J. M. Jabbour, K. C. Maitland, J. Wright, Y. S. L. Cheng, and J. A. Jo, “Handheld multispectral fluorescence lifetime imaging system for in vivo applications,” Biomed. Opt. Express 5(3), 921–931 (2014).
[Crossref] [PubMed]

A. S. Kristoffersen, S. R. Erga, B. Hamre, and Ø. Frette, “Testing Fluorescence Lifetime Standards using Two-Photon Excitation and Time-Domain Instrumentation: Rhodamine B, Coumarin 6 and Lucifer Yellow,” J. Fluoresc. 24(4), 1015–1024 (2014).
[Crossref] [PubMed]

2013 (5)

Y. Dancik, A. Favre, C. J. Loy, A. V. Zvyagin, and M. S. Roberts, “Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentation in vivo,” J. Biomed. Opt. 18(2), 026022 (2013).
[Crossref] [PubMed]

M. S. Islam, M. Honma, T. Nakabayashi, M. Kinjo, and N. Ohta, “pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells,” Int. J. Mol. Sci. 14(1), 1952–1963 (2013).
[Crossref] [PubMed]

J. M. Jabbour, S. Cheng, B. H. Malik, R. Cuenca, J. A. Jo, J. Wright, Y. S. L. Cheng, and K. C. Maitland, “Fluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer,” J. Biomed. Opt. 18(4), 046012 (2013).
[Crossref] [PubMed]

S. Cheng, J. J. Rico-Jimenez, J. Jabbour, B. Malik, K. C. Maitland, J. Wright, Y. S. L. Cheng, and J. A. Jo, “Flexible endoscope for continuous in vivo multispectral fluorescence lifetime imaging,” Opt. Lett. 38(9), 1515–1517 (2013).
[Crossref] [PubMed]

J. L. Rinnenthal, C. Börnchen, H. Radbruch, V. Andresen, A. Mossakowski, V. Siffrin, T. Seelemann, H. Spiecker, I. Moll, J. Herz, A. E. Hauser, F. Zipp, M. J. Behne, and R. Niesner, “Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation,” PLoS One 8(4), e60100 (2013).
[Crossref] [PubMed]

2012 (3)

J. Park, P. Pande, S. Shrestha, F. Clubb, B. E. Applegate, and J. A. Jo, “Biochemical characterization of atherosclerotic plaques by endogenous multispectral fluorescence lifetime imaging microscopy,” Atherosclerosis 220(2), 394–401 (2012).
[Crossref] [PubMed]

W. Becker, “Fluorescence lifetime imaging--techniques and applications,” J. Microsc. 247(2), 119–136 (2012).
[Crossref] [PubMed]

I. Pavlova, K. R. Hume, S. A. Yazinski, J. Flanders, T. L. Southard, R. S. Weiss, and W. W. Webb, “Multiphoton microscopy and microspectroscopy for diagnostics of inflammatory and neoplastic lung,” J. Biomed. Opt. 17(3), 036014 (2012).
[Crossref] [PubMed]

2011 (2)

2010 (4)

Y. J. Won, S. Moon, W. T. Han, and D. Y. Kim, “Referencing techniques for the analog mean-delay method in fluorescence lifetime imaging,” J. Opt. Soc. Am. A 27(11), 2402–2410 (2010).
[Crossref] [PubMed]

A. F. Tarantal, H. Chen, T. T. Shi, C. H. Lu, A. B. Fang, S. Buckley, M. Kolb, J. Gauldie, D. Warburton, and W. Shi, “Overexpression of transforming growth factor-beta1 in fetal monkey lung results in prenatal pulmonary fibrosis,” Eur. Respir. J. 36(4), 907–914 (2010).
[Crossref] [PubMed]

C. Li, C. Pitsillides, J. M. Runnels, D. Côté, and C. P. Lin, “Multiphoton Microscopy of Live Tissues With Ultraviolet Autofluorescence,” IEEE J. Sel. Top. Quantum Electron 16(3), 516–523 (2010).
[Crossref]

M. Y. Berezin and S. Achilefu, “Fluorescence Lifetime Measurements and Biological Imaging,” Chem. Rev. 110(5), 2641–2684 (2010).
[Crossref] [PubMed]

2009 (1)

2008 (1)

N. P. Galletly, J. McGinty, C. Dunsby, F. Teixeira, J. Requejo-Isidro, I. Munro, D. S. Elson, M. A. Neil, A. C. Chu, P. M. French, and G. W. Stamp, “Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin,” Br. J. Dermatol. 159(1), 152–161 (2008).
[Crossref] [PubMed]

2003 (1)

P. Kapusta, R. Erdmann, U. Ortmann, and M. Wahl, “Time-resolved fluorescence anisotropy measurements made simple,” J. Fluoresc. 13(2), 179–183 (2003).
[Crossref]

2001 (1)

H. Fehrenbach, “Alveolar epithelial type II cell: defender of the alveolus revisited,” Respir. Res. 2(1), 33–46 (2001).
[Crossref] [PubMed]

1999 (1)

P. I. H. Bastiaens and A. Squire, “Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell,” Trends Cell Biol. 9(2), 48–52 (1999).
[Crossref] [PubMed]

1997 (1)

N. Barkai and S. Leibler, “Robustness in simple biochemical networks,” Nature 387(6636), 913–917 (1997).
[Crossref] [PubMed]

1995 (1)

D. Bray, “Protein Molecules as Computational Elements in Living Cells,” Nature 376(6538), 307–312 (1995).
[Crossref] [PubMed]

1992 (1)

J. R. Lakowicz, H. Szmacinski, K. Nowaczyk, and M. L. Johnson, “Fluorescence lifetime imaging of free and protein-bound NADH,” Proc. Natl. Acad. Sci. U.S.A. 89(4), 1271–1275 (1992).
[Crossref] [PubMed]

Achilefu, S.

M. Y. Berezin and S. Achilefu, “Fluorescence Lifetime Measurements and Biological Imaging,” Chem. Rev. 110(5), 2641–2684 (2010).
[Crossref] [PubMed]

Ahmed, B.

B. H. Malik, J. Lee, S. Cheng, R. Cuenca, J. M. Jabbour, Y. S. L. Cheng, J. M. Wright, B. Ahmed, K. C. Maitland, and J. A. Jo, “Objective Detection of Oral Carcinoma with Multispectral Fluorescence Lifetime Imaging In Vivo,” Photochem. Photobiol. 92(5), 694–701 (2016).
[Crossref] [PubMed]

Andresen, V.

J. L. Rinnenthal, C. Börnchen, H. Radbruch, V. Andresen, A. Mossakowski, V. Siffrin, T. Seelemann, H. Spiecker, I. Moll, J. Herz, A. E. Hauser, F. Zipp, M. J. Behne, and R. Niesner, “Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation,” PLoS One 8(4), e60100 (2013).
[Crossref] [PubMed]

Applegate, B. E.

J. Park, P. Pande, S. Shrestha, F. Clubb, B. E. Applegate, and J. A. Jo, “Biochemical characterization of atherosclerotic plaques by endogenous multispectral fluorescence lifetime imaging microscopy,” Atherosclerosis 220(2), 394–401 (2012).
[Crossref] [PubMed]

Barkai, N.

N. Barkai and S. Leibler, “Robustness in simple biochemical networks,” Nature 387(6636), 913–917 (1997).
[Crossref] [PubMed]

Bastiaens, P. I. H.

P. I. H. Bastiaens and A. Squire, “Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell,” Trends Cell Biol. 9(2), 48–52 (1999).
[Crossref] [PubMed]

Becker, W.

W. Becker, “Fluorescence lifetime imaging--techniques and applications,” J. Microsc. 247(2), 119–136 (2012).
[Crossref] [PubMed]

Behne, M. J.

J. L. Rinnenthal, C. Börnchen, H. Radbruch, V. Andresen, A. Mossakowski, V. Siffrin, T. Seelemann, H. Spiecker, I. Moll, J. Herz, A. E. Hauser, F. Zipp, M. J. Behne, and R. Niesner, “Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation,” PLoS One 8(4), e60100 (2013).
[Crossref] [PubMed]

Berezin, M. Y.

M. Y. Berezin and S. Achilefu, “Fluorescence Lifetime Measurements and Biological Imaging,” Chem. Rev. 110(5), 2641–2684 (2010).
[Crossref] [PubMed]

Börnchen, C.

J. L. Rinnenthal, C. Börnchen, H. Radbruch, V. Andresen, A. Mossakowski, V. Siffrin, T. Seelemann, H. Spiecker, I. Moll, J. Herz, A. E. Hauser, F. Zipp, M. J. Behne, and R. Niesner, “Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation,” PLoS One 8(4), e60100 (2013).
[Crossref] [PubMed]

Bray, D.

D. Bray, “Protein Molecules as Computational Elements in Living Cells,” Nature 376(6538), 307–312 (1995).
[Crossref] [PubMed]

Buckley, S.

A. F. Tarantal, H. Chen, T. T. Shi, C. H. Lu, A. B. Fang, S. Buckley, M. Kolb, J. Gauldie, D. Warburton, and W. Shi, “Overexpression of transforming growth factor-beta1 in fetal monkey lung results in prenatal pulmonary fibrosis,” Eur. Respir. J. 36(4), 907–914 (2010).
[Crossref] [PubMed]

Chen, H.

A. F. Tarantal, H. Chen, T. T. Shi, C. H. Lu, A. B. Fang, S. Buckley, M. Kolb, J. Gauldie, D. Warburton, and W. Shi, “Overexpression of transforming growth factor-beta1 in fetal monkey lung results in prenatal pulmonary fibrosis,” Eur. Respir. J. 36(4), 907–914 (2010).
[Crossref] [PubMed]

Cheng, S.

B. H. Malik, J. Lee, S. Cheng, R. Cuenca, J. M. Jabbour, Y. S. L. Cheng, J. M. Wright, B. Ahmed, K. C. Maitland, and J. A. Jo, “Objective Detection of Oral Carcinoma with Multispectral Fluorescence Lifetime Imaging In Vivo,” Photochem. Photobiol. 92(5), 694–701 (2016).
[Crossref] [PubMed]

S. Cheng, R. M. Cuenca, B. Liu, B. H. Malik, J. M. Jabbour, K. C. Maitland, J. Wright, Y. S. L. Cheng, and J. A. Jo, “Handheld multispectral fluorescence lifetime imaging system for in vivo applications,” Biomed. Opt. Express 5(3), 921–931 (2014).
[Crossref] [PubMed]

J. M. Jabbour, S. Cheng, B. H. Malik, R. Cuenca, J. A. Jo, J. Wright, Y. S. L. Cheng, and K. C. Maitland, “Fluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer,” J. Biomed. Opt. 18(4), 046012 (2013).
[Crossref] [PubMed]

S. Cheng, J. J. Rico-Jimenez, J. Jabbour, B. Malik, K. C. Maitland, J. Wright, Y. S. L. Cheng, and J. A. Jo, “Flexible endoscope for continuous in vivo multispectral fluorescence lifetime imaging,” Opt. Lett. 38(9), 1515–1517 (2013).
[Crossref] [PubMed]

Cheng, Y. S. L.

B. H. Malik, J. Lee, S. Cheng, R. Cuenca, J. M. Jabbour, Y. S. L. Cheng, J. M. Wright, B. Ahmed, K. C. Maitland, and J. A. Jo, “Objective Detection of Oral Carcinoma with Multispectral Fluorescence Lifetime Imaging In Vivo,” Photochem. Photobiol. 92(5), 694–701 (2016).
[Crossref] [PubMed]

S. Cheng, R. M. Cuenca, B. Liu, B. H. Malik, J. M. Jabbour, K. C. Maitland, J. Wright, Y. S. L. Cheng, and J. A. Jo, “Handheld multispectral fluorescence lifetime imaging system for in vivo applications,” Biomed. Opt. Express 5(3), 921–931 (2014).
[Crossref] [PubMed]

J. M. Jabbour, S. Cheng, B. H. Malik, R. Cuenca, J. A. Jo, J. Wright, Y. S. L. Cheng, and K. C. Maitland, “Fluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer,” J. Biomed. Opt. 18(4), 046012 (2013).
[Crossref] [PubMed]

S. Cheng, J. J. Rico-Jimenez, J. Jabbour, B. Malik, K. C. Maitland, J. Wright, Y. S. L. Cheng, and J. A. Jo, “Flexible endoscope for continuous in vivo multispectral fluorescence lifetime imaging,” Opt. Lett. 38(9), 1515–1517 (2013).
[Crossref] [PubMed]

Chu, A. C.

N. P. Galletly, J. McGinty, C. Dunsby, F. Teixeira, J. Requejo-Isidro, I. Munro, D. S. Elson, M. A. Neil, A. C. Chu, P. M. French, and G. W. Stamp, “Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin,” Br. J. Dermatol. 159(1), 152–161 (2008).
[Crossref] [PubMed]

Clubb, F.

J. Park, P. Pande, S. Shrestha, F. Clubb, B. E. Applegate, and J. A. Jo, “Biochemical characterization of atherosclerotic plaques by endogenous multispectral fluorescence lifetime imaging microscopy,” Atherosclerosis 220(2), 394–401 (2012).
[Crossref] [PubMed]

Connolly, J. L.

Côté, D.

C. Li, C. Pitsillides, J. M. Runnels, D. Côté, and C. P. Lin, “Multiphoton Microscopy of Live Tissues With Ultraviolet Autofluorescence,” IEEE J. Sel. Top. Quantum Electron 16(3), 516–523 (2010).
[Crossref]

Cuenca, R.

B. H. Malik, J. Lee, S. Cheng, R. Cuenca, J. M. Jabbour, Y. S. L. Cheng, J. M. Wright, B. Ahmed, K. C. Maitland, and J. A. Jo, “Objective Detection of Oral Carcinoma with Multispectral Fluorescence Lifetime Imaging In Vivo,” Photochem. Photobiol. 92(5), 694–701 (2016).
[Crossref] [PubMed]

J. M. Jabbour, S. Cheng, B. H. Malik, R. Cuenca, J. A. Jo, J. Wright, Y. S. L. Cheng, and K. C. Maitland, “Fluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer,” J. Biomed. Opt. 18(4), 046012 (2013).
[Crossref] [PubMed]

Cuenca, R. M.

Dagher, P. C.

T. Hato, S. Winfree, R. Day, R. M. Sandoval, B. A. Molitoris, M. C. Yoder, R. C. Wiggins, Y. Zheng, K. W. Dunn, and P. C. Dagher, “Two-Photon Intravital Fluorescence Lifetime Imaging of the Kidney Reveals Cell-Type Specific Metabolic Signatures,” J. Am. Soc. Nephrol. 28(8), 2420–2430 (2017).
[Crossref] [PubMed]

Dancik, Y.

Y. Dancik, A. Favre, C. J. Loy, A. V. Zvyagin, and M. S. Roberts, “Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentation in vivo,” J. Biomed. Opt. 18(2), 026022 (2013).
[Crossref] [PubMed]

Day, R.

T. Hato, S. Winfree, R. Day, R. M. Sandoval, B. A. Molitoris, M. C. Yoder, R. C. Wiggins, Y. Zheng, K. W. Dunn, and P. C. Dagher, “Two-Photon Intravital Fluorescence Lifetime Imaging of the Kidney Reveals Cell-Type Specific Metabolic Signatures,” J. Am. Soc. Nephrol. 28(8), 2420–2430 (2017).
[Crossref] [PubMed]

Dow, X. Y.

Dunn, K. W.

T. Hato, S. Winfree, R. Day, R. M. Sandoval, B. A. Molitoris, M. C. Yoder, R. C. Wiggins, Y. Zheng, K. W. Dunn, and P. C. Dagher, “Two-Photon Intravital Fluorescence Lifetime Imaging of the Kidney Reveals Cell-Type Specific Metabolic Signatures,” J. Am. Soc. Nephrol. 28(8), 2420–2430 (2017).
[Crossref] [PubMed]

Dunsby, C.

N. P. Galletly, J. McGinty, C. Dunsby, F. Teixeira, J. Requejo-Isidro, I. Munro, D. S. Elson, M. A. Neil, A. C. Chu, P. M. French, and G. W. Stamp, “Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin,” Br. J. Dermatol. 159(1), 152–161 (2008).
[Crossref] [PubMed]

Elson, D. S.

N. P. Galletly, J. McGinty, C. Dunsby, F. Teixeira, J. Requejo-Isidro, I. Munro, D. S. Elson, M. A. Neil, A. C. Chu, P. M. French, and G. W. Stamp, “Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin,” Br. J. Dermatol. 159(1), 152–161 (2008).
[Crossref] [PubMed]

Erdmann, R.

P. Kapusta, R. Erdmann, U. Ortmann, and M. Wahl, “Time-resolved fluorescence anisotropy measurements made simple,” J. Fluoresc. 13(2), 179–183 (2003).
[Crossref]

Erga, S. R.

A. S. Kristoffersen, S. R. Erga, B. Hamre, and Ø. Frette, “Testing Fluorescence Lifetime Standards using Two-Photon Excitation and Time-Domain Instrumentation: Rhodamine B, Coumarin 6 and Lucifer Yellow,” J. Fluoresc. 24(4), 1015–1024 (2014).
[Crossref] [PubMed]

Fang, A. B.

A. F. Tarantal, H. Chen, T. T. Shi, C. H. Lu, A. B. Fang, S. Buckley, M. Kolb, J. Gauldie, D. Warburton, and W. Shi, “Overexpression of transforming growth factor-beta1 in fetal monkey lung results in prenatal pulmonary fibrosis,” Eur. Respir. J. 36(4), 907–914 (2010).
[Crossref] [PubMed]

Favre, A.

Y. Dancik, A. Favre, C. J. Loy, A. V. Zvyagin, and M. S. Roberts, “Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentation in vivo,” J. Biomed. Opt. 18(2), 026022 (2013).
[Crossref] [PubMed]

Fehrenbach, H.

H. Fehrenbach, “Alveolar epithelial type II cell: defender of the alveolus revisited,” Respir. Res. 2(1), 33–46 (2001).
[Crossref] [PubMed]

Flanders, J.

I. Pavlova, K. R. Hume, S. A. Yazinski, J. Flanders, T. L. Southard, R. S. Weiss, and W. W. Webb, “Multiphoton microscopy and microspectroscopy for diagnostics of inflammatory and neoplastic lung,” J. Biomed. Opt. 17(3), 036014 (2012).
[Crossref] [PubMed]

French, P. M.

N. P. Galletly, J. McGinty, C. Dunsby, F. Teixeira, J. Requejo-Isidro, I. Munro, D. S. Elson, M. A. Neil, A. C. Chu, P. M. French, and G. W. Stamp, “Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin,” Br. J. Dermatol. 159(1), 152–161 (2008).
[Crossref] [PubMed]

Frette, Ø.

A. S. Kristoffersen, S. R. Erga, B. Hamre, and Ø. Frette, “Testing Fluorescence Lifetime Standards using Two-Photon Excitation and Time-Domain Instrumentation: Rhodamine B, Coumarin 6 and Lucifer Yellow,” J. Fluoresc. 24(4), 1015–1024 (2014).
[Crossref] [PubMed]

Fujimoto, J. G.

Galletly, N. P.

N. P. Galletly, J. McGinty, C. Dunsby, F. Teixeira, J. Requejo-Isidro, I. Munro, D. S. Elson, M. A. Neil, A. C. Chu, P. M. French, and G. W. Stamp, “Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin,” Br. J. Dermatol. 159(1), 152–161 (2008).
[Crossref] [PubMed]

Gauldie, J.

A. F. Tarantal, H. Chen, T. T. Shi, C. H. Lu, A. B. Fang, S. Buckley, M. Kolb, J. Gauldie, D. Warburton, and W. Shi, “Overexpression of transforming growth factor-beta1 in fetal monkey lung results in prenatal pulmonary fibrosis,” Eur. Respir. J. 36(4), 907–914 (2010).
[Crossref] [PubMed]

Giacomelli, M. G.

Gweon, D.

J. Kim, J. Ryu, and D. Gweon, “Real-time Fluorescence Lifetime Imaging Microscopy Implementation by Analog Mean-Delay Method through Parallel Data Processing,” Applied Microscopy 46(1), 6–13 (2016).
[Crossref]

Gweon, D. G.

Hamre, B.

A. S. Kristoffersen, S. R. Erga, B. Hamre, and Ø. Frette, “Testing Fluorescence Lifetime Standards using Two-Photon Excitation and Time-Domain Instrumentation: Rhodamine B, Coumarin 6 and Lucifer Yellow,” J. Fluoresc. 24(4), 1015–1024 (2014).
[Crossref] [PubMed]

Han, W. T.

Hato, T.

T. Hato, S. Winfree, R. Day, R. M. Sandoval, B. A. Molitoris, M. C. Yoder, R. C. Wiggins, Y. Zheng, K. W. Dunn, and P. C. Dagher, “Two-Photon Intravital Fluorescence Lifetime Imaging of the Kidney Reveals Cell-Type Specific Metabolic Signatures,” J. Am. Soc. Nephrol. 28(8), 2420–2430 (2017).
[Crossref] [PubMed]

Hauser, A. E.

J. L. Rinnenthal, C. Börnchen, H. Radbruch, V. Andresen, A. Mossakowski, V. Siffrin, T. Seelemann, H. Spiecker, I. Moll, J. Herz, A. E. Hauser, F. Zipp, M. J. Behne, and R. Niesner, “Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation,” PLoS One 8(4), e60100 (2013).
[Crossref] [PubMed]

Herz, J.

J. L. Rinnenthal, C. Börnchen, H. Radbruch, V. Andresen, A. Mossakowski, V. Siffrin, T. Seelemann, H. Spiecker, I. Moll, J. Herz, A. E. Hauser, F. Zipp, M. J. Behne, and R. Niesner, “Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation,” PLoS One 8(4), e60100 (2013).
[Crossref] [PubMed]

Hinsdale, T.

Honma, M.

M. S. Islam, M. Honma, T. Nakabayashi, M. Kinjo, and N. Ohta, “pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells,” Int. J. Mol. Sci. 14(1), 1952–1963 (2013).
[Crossref] [PubMed]

Hume, K. R.

I. Pavlova, K. R. Hume, S. A. Yazinski, J. Flanders, T. L. Southard, R. S. Weiss, and W. W. Webb, “Multiphoton microscopy and microspectroscopy for diagnostics of inflammatory and neoplastic lung,” J. Biomed. Opt. 17(3), 036014 (2012).
[Crossref] [PubMed]

Islam, M. S.

M. S. Islam, M. Honma, T. Nakabayashi, M. Kinjo, and N. Ohta, “pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells,” Int. J. Mol. Sci. 14(1), 1952–1963 (2013).
[Crossref] [PubMed]

Jabbour, J.

Jabbour, J. M.

B. H. Malik, J. Lee, S. Cheng, R. Cuenca, J. M. Jabbour, Y. S. L. Cheng, J. M. Wright, B. Ahmed, K. C. Maitland, and J. A. Jo, “Objective Detection of Oral Carcinoma with Multispectral Fluorescence Lifetime Imaging In Vivo,” Photochem. Photobiol. 92(5), 694–701 (2016).
[Crossref] [PubMed]

S. Cheng, R. M. Cuenca, B. Liu, B. H. Malik, J. M. Jabbour, K. C. Maitland, J. Wright, Y. S. L. Cheng, and J. A. Jo, “Handheld multispectral fluorescence lifetime imaging system for in vivo applications,” Biomed. Opt. Express 5(3), 921–931 (2014).
[Crossref] [PubMed]

J. M. Jabbour, S. Cheng, B. H. Malik, R. Cuenca, J. A. Jo, J. Wright, Y. S. L. Cheng, and K. C. Maitland, “Fluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer,” J. Biomed. Opt. 18(4), 046012 (2013).
[Crossref] [PubMed]

Jeong, J. H.

Jo, J. A.

T. Hinsdale, C. Olsovsky, J. J. Rico-Jimenez, K. C. Maitland, J. A. Jo, and B. H. Malik, “Optically sectioned wide-field fluorescence lifetime imaging microscopy enabled by structured illumination,” Biomed. Opt. Express 8(3), 1455–1465 (2017).
[Crossref] [PubMed]

B. H. Malik, J. Lee, S. Cheng, R. Cuenca, J. M. Jabbour, Y. S. L. Cheng, J. M. Wright, B. Ahmed, K. C. Maitland, and J. A. Jo, “Objective Detection of Oral Carcinoma with Multispectral Fluorescence Lifetime Imaging In Vivo,” Photochem. Photobiol. 92(5), 694–701 (2016).
[Crossref] [PubMed]

S. Cheng, R. M. Cuenca, B. Liu, B. H. Malik, J. M. Jabbour, K. C. Maitland, J. Wright, Y. S. L. Cheng, and J. A. Jo, “Handheld multispectral fluorescence lifetime imaging system for in vivo applications,” Biomed. Opt. Express 5(3), 921–931 (2014).
[Crossref] [PubMed]

J. M. Jabbour, S. Cheng, B. H. Malik, R. Cuenca, J. A. Jo, J. Wright, Y. S. L. Cheng, and K. C. Maitland, “Fluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer,” J. Biomed. Opt. 18(4), 046012 (2013).
[Crossref] [PubMed]

S. Cheng, J. J. Rico-Jimenez, J. Jabbour, B. Malik, K. C. Maitland, J. Wright, Y. S. L. Cheng, and J. A. Jo, “Flexible endoscope for continuous in vivo multispectral fluorescence lifetime imaging,” Opt. Lett. 38(9), 1515–1517 (2013).
[Crossref] [PubMed]

J. Park, P. Pande, S. Shrestha, F. Clubb, B. E. Applegate, and J. A. Jo, “Biochemical characterization of atherosclerotic plaques by endogenous multispectral fluorescence lifetime imaging microscopy,” Atherosclerosis 220(2), 394–401 (2012).
[Crossref] [PubMed]

Johnson, M. L.

J. R. Lakowicz, H. Szmacinski, K. Nowaczyk, and M. L. Johnson, “Fluorescence lifetime imaging of free and protein-bound NADH,” Proc. Natl. Acad. Sci. U.S.A. 89(4), 1271–1275 (1992).
[Crossref] [PubMed]

Kang, W. J.

Kapusta, P.

P. Kapusta, R. Erdmann, U. Ortmann, and M. Wahl, “Time-resolved fluorescence anisotropy measurements made simple,” J. Fluoresc. 13(2), 179–183 (2003).
[Crossref]

Kim, B.

Kim, D.

Kim, D. Y.

Kim, H.

Kim, J.

J. Ryu, J. Kim, H. Kim, J. H. Jeong, H. J. Lee, H. Yoo, and D. G. Gweon, “High-speed time-resolved laser-scanning microscopy using the line-to-pixel referencing method,” Appl. Opt. 55(32), 9033–9041 (2016).
[Crossref] [PubMed]

J. Kim, J. Ryu, and D. Gweon, “Real-time Fluorescence Lifetime Imaging Microscopy Implementation by Analog Mean-Delay Method through Parallel Data Processing,” Applied Microscopy 46(1), 6–13 (2016).
[Crossref]

Kim, J. W.

Kinjo, M.

M. S. Islam, M. Honma, T. Nakabayashi, M. Kinjo, and N. Ohta, “pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells,” Int. J. Mol. Sci. 14(1), 1952–1963 (2013).
[Crossref] [PubMed]

Kolb, M.

A. F. Tarantal, H. Chen, T. T. Shi, C. H. Lu, A. B. Fang, S. Buckley, M. Kolb, J. Gauldie, D. Warburton, and W. Shi, “Overexpression of transforming growth factor-beta1 in fetal monkey lung results in prenatal pulmonary fibrosis,” Eur. Respir. J. 36(4), 907–914 (2010).
[Crossref] [PubMed]

Kristoffersen, A. S.

A. S. Kristoffersen, S. R. Erga, B. Hamre, and Ø. Frette, “Testing Fluorescence Lifetime Standards using Two-Photon Excitation and Time-Domain Instrumentation: Rhodamine B, Coumarin 6 and Lucifer Yellow,” J. Fluoresc. 24(4), 1015–1024 (2014).
[Crossref] [PubMed]

Lakowicz, J. R.

J. R. Lakowicz, H. Szmacinski, K. Nowaczyk, and M. L. Johnson, “Fluorescence lifetime imaging of free and protein-bound NADH,” Proc. Natl. Acad. Sci. U.S.A. 89(4), 1271–1275 (1992).
[Crossref] [PubMed]

Lee, H. J.

Lee, J.

B. H. Malik, J. Lee, S. Cheng, R. Cuenca, J. M. Jabbour, Y. S. L. Cheng, J. M. Wright, B. Ahmed, K. C. Maitland, and J. A. Jo, “Objective Detection of Oral Carcinoma with Multispectral Fluorescence Lifetime Imaging In Vivo,” Photochem. Photobiol. 92(5), 694–701 (2016).
[Crossref] [PubMed]

Lee, M. W.

Lee, S.

Leibler, S.

N. Barkai and S. Leibler, “Robustness in simple biochemical networks,” Nature 387(6636), 913–917 (1997).
[Crossref] [PubMed]

Li, C.

C. Li, C. Pitsillides, J. M. Runnels, D. Côté, and C. P. Lin, “Multiphoton Microscopy of Live Tissues With Ultraviolet Autofluorescence,” IEEE J. Sel. Top. Quantum Electron 16(3), 516–523 (2010).
[Crossref]

Lin, C. P.

C. Li, C. Pitsillides, J. M. Runnels, D. Côté, and C. P. Lin, “Multiphoton Microscopy of Live Tissues With Ultraviolet Autofluorescence,” IEEE J. Sel. Top. Quantum Electron 16(3), 516–523 (2010).
[Crossref]

Liu, B.

Loy, C. J.

Y. Dancik, A. Favre, C. J. Loy, A. V. Zvyagin, and M. S. Roberts, “Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentation in vivo,” J. Biomed. Opt. 18(2), 026022 (2013).
[Crossref] [PubMed]

Lu, C. H.

A. F. Tarantal, H. Chen, T. T. Shi, C. H. Lu, A. B. Fang, S. Buckley, M. Kolb, J. Gauldie, D. Warburton, and W. Shi, “Overexpression of transforming growth factor-beta1 in fetal monkey lung results in prenatal pulmonary fibrosis,” Eur. Respir. J. 36(4), 907–914 (2010).
[Crossref] [PubMed]

Maitland, K. C.

Malik, B.

Malik, B. H.

T. Hinsdale, C. Olsovsky, J. J. Rico-Jimenez, K. C. Maitland, J. A. Jo, and B. H. Malik, “Optically sectioned wide-field fluorescence lifetime imaging microscopy enabled by structured illumination,” Biomed. Opt. Express 8(3), 1455–1465 (2017).
[Crossref] [PubMed]

B. H. Malik, J. Lee, S. Cheng, R. Cuenca, J. M. Jabbour, Y. S. L. Cheng, J. M. Wright, B. Ahmed, K. C. Maitland, and J. A. Jo, “Objective Detection of Oral Carcinoma with Multispectral Fluorescence Lifetime Imaging In Vivo,” Photochem. Photobiol. 92(5), 694–701 (2016).
[Crossref] [PubMed]

S. Cheng, R. M. Cuenca, B. Liu, B. H. Malik, J. M. Jabbour, K. C. Maitland, J. Wright, Y. S. L. Cheng, and J. A. Jo, “Handheld multispectral fluorescence lifetime imaging system for in vivo applications,” Biomed. Opt. Express 5(3), 921–931 (2014).
[Crossref] [PubMed]

J. M. Jabbour, S. Cheng, B. H. Malik, R. Cuenca, J. A. Jo, J. Wright, Y. S. L. Cheng, and K. C. Maitland, “Fluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer,” J. Biomed. Opt. 18(4), 046012 (2013).
[Crossref] [PubMed]

McGinty, J.

N. P. Galletly, J. McGinty, C. Dunsby, F. Teixeira, J. Requejo-Isidro, I. Munro, D. S. Elson, M. A. Neil, A. C. Chu, P. M. French, and G. W. Stamp, “Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin,” Br. J. Dermatol. 159(1), 152–161 (2008).
[Crossref] [PubMed]

Molitoris, B. A.

T. Hato, S. Winfree, R. Day, R. M. Sandoval, B. A. Molitoris, M. C. Yoder, R. C. Wiggins, Y. Zheng, K. W. Dunn, and P. C. Dagher, “Two-Photon Intravital Fluorescence Lifetime Imaging of the Kidney Reveals Cell-Type Specific Metabolic Signatures,” J. Am. Soc. Nephrol. 28(8), 2420–2430 (2017).
[Crossref] [PubMed]

Moll, I.

J. L. Rinnenthal, C. Börnchen, H. Radbruch, V. Andresen, A. Mossakowski, V. Siffrin, T. Seelemann, H. Spiecker, I. Moll, J. Herz, A. E. Hauser, F. Zipp, M. J. Behne, and R. Niesner, “Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation,” PLoS One 8(4), e60100 (2013).
[Crossref] [PubMed]

Moon, S.

Mossakowski, A.

J. L. Rinnenthal, C. Börnchen, H. Radbruch, V. Andresen, A. Mossakowski, V. Siffrin, T. Seelemann, H. Spiecker, I. Moll, J. Herz, A. E. Hauser, F. Zipp, M. J. Behne, and R. Niesner, “Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation,” PLoS One 8(4), e60100 (2013).
[Crossref] [PubMed]

Muir, R. D.

Munro, I.

N. P. Galletly, J. McGinty, C. Dunsby, F. Teixeira, J. Requejo-Isidro, I. Munro, D. S. Elson, M. A. Neil, A. C. Chu, P. M. French, and G. W. Stamp, “Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin,” Br. J. Dermatol. 159(1), 152–161 (2008).
[Crossref] [PubMed]

Nakabayashi, T.

M. S. Islam, M. Honma, T. Nakabayashi, M. Kinjo, and N. Ohta, “pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells,” Int. J. Mol. Sci. 14(1), 1952–1963 (2013).
[Crossref] [PubMed]

Nam, H. S.

Neil, M. A.

N. P. Galletly, J. McGinty, C. Dunsby, F. Teixeira, J. Requejo-Isidro, I. Munro, D. S. Elson, M. A. Neil, A. C. Chu, P. M. French, and G. W. Stamp, “Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin,” Br. J. Dermatol. 159(1), 152–161 (2008).
[Crossref] [PubMed]

Niesner, R.

J. L. Rinnenthal, C. Börnchen, H. Radbruch, V. Andresen, A. Mossakowski, V. Siffrin, T. Seelemann, H. Spiecker, I. Moll, J. Herz, A. E. Hauser, F. Zipp, M. J. Behne, and R. Niesner, “Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation,” PLoS One 8(4), e60100 (2013).
[Crossref] [PubMed]

Nowaczyk, K.

J. R. Lakowicz, H. Szmacinski, K. Nowaczyk, and M. L. Johnson, “Fluorescence lifetime imaging of free and protein-bound NADH,” Proc. Natl. Acad. Sci. U.S.A. 89(4), 1271–1275 (1992).
[Crossref] [PubMed]

Oh, W. Y.

Ohta, N.

M. S. Islam, M. Honma, T. Nakabayashi, M. Kinjo, and N. Ohta, “pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells,” Int. J. Mol. Sci. 14(1), 1952–1963 (2013).
[Crossref] [PubMed]

Olsovsky, C.

Ortmann, U.

P. Kapusta, R. Erdmann, U. Ortmann, and M. Wahl, “Time-resolved fluorescence anisotropy measurements made simple,” J. Fluoresc. 13(2), 179–183 (2003).
[Crossref]

Pande, P.

J. Park, P. Pande, S. Shrestha, F. Clubb, B. E. Applegate, and J. A. Jo, “Biochemical characterization of atherosclerotic plaques by endogenous multispectral fluorescence lifetime imaging microscopy,” Atherosclerosis 220(2), 394–401 (2012).
[Crossref] [PubMed]

Park, B.

Park, J.

J. Park, P. Pande, S. Shrestha, F. Clubb, B. E. Applegate, and J. A. Jo, “Biochemical characterization of atherosclerotic plaques by endogenous multispectral fluorescence lifetime imaging microscopy,” Atherosclerosis 220(2), 394–401 (2012).
[Crossref] [PubMed]

Pavlova, I.

I. Pavlova, K. R. Hume, S. A. Yazinski, J. Flanders, T. L. Southard, R. S. Weiss, and W. W. Webb, “Multiphoton microscopy and microspectroscopy for diagnostics of inflammatory and neoplastic lung,” J. Biomed. Opt. 17(3), 036014 (2012).
[Crossref] [PubMed]

Pitsillides, C.

C. Li, C. Pitsillides, J. M. Runnels, D. Côté, and C. P. Lin, “Multiphoton Microscopy of Live Tissues With Ultraviolet Autofluorescence,” IEEE J. Sel. Top. Quantum Electron 16(3), 516–523 (2010).
[Crossref]

Radbruch, H.

J. L. Rinnenthal, C. Börnchen, H. Radbruch, V. Andresen, A. Mossakowski, V. Siffrin, T. Seelemann, H. Spiecker, I. Moll, J. Herz, A. E. Hauser, F. Zipp, M. J. Behne, and R. Niesner, “Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation,” PLoS One 8(4), e60100 (2013).
[Crossref] [PubMed]

Requejo-Isidro, J.

N. P. Galletly, J. McGinty, C. Dunsby, F. Teixeira, J. Requejo-Isidro, I. Munro, D. S. Elson, M. A. Neil, A. C. Chu, P. M. French, and G. W. Stamp, “Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin,” Br. J. Dermatol. 159(1), 152–161 (2008).
[Crossref] [PubMed]

Rico-Jimenez, J. J.

Rinnenthal, J. L.

J. L. Rinnenthal, C. Börnchen, H. Radbruch, V. Andresen, A. Mossakowski, V. Siffrin, T. Seelemann, H. Spiecker, I. Moll, J. Herz, A. E. Hauser, F. Zipp, M. J. Behne, and R. Niesner, “Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation,” PLoS One 8(4), e60100 (2013).
[Crossref] [PubMed]

Roberts, M. S.

Y. Dancik, A. Favre, C. J. Loy, A. V. Zvyagin, and M. S. Roberts, “Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentation in vivo,” J. Biomed. Opt. 18(2), 026022 (2013).
[Crossref] [PubMed]

Runnels, J. M.

C. Li, C. Pitsillides, J. M. Runnels, D. Côté, and C. P. Lin, “Multiphoton Microscopy of Live Tissues With Ultraviolet Autofluorescence,” IEEE J. Sel. Top. Quantum Electron 16(3), 516–523 (2010).
[Crossref]

Ryu, J.

J. Kim, J. Ryu, and D. Gweon, “Real-time Fluorescence Lifetime Imaging Microscopy Implementation by Analog Mean-Delay Method through Parallel Data Processing,” Applied Microscopy 46(1), 6–13 (2016).
[Crossref]

J. Ryu, J. Kim, H. Kim, J. H. Jeong, H. J. Lee, H. Yoo, and D. G. Gweon, “High-speed time-resolved laser-scanning microscopy using the line-to-pixel referencing method,” Appl. Opt. 55(32), 9033–9041 (2016).
[Crossref] [PubMed]

Sandoval, R. M.

T. Hato, S. Winfree, R. Day, R. M. Sandoval, B. A. Molitoris, M. C. Yoder, R. C. Wiggins, Y. Zheng, K. W. Dunn, and P. C. Dagher, “Two-Photon Intravital Fluorescence Lifetime Imaging of the Kidney Reveals Cell-Type Specific Metabolic Signatures,” J. Am. Soc. Nephrol. 28(8), 2420–2430 (2017).
[Crossref] [PubMed]

Seelemann, T.

J. L. Rinnenthal, C. Börnchen, H. Radbruch, V. Andresen, A. Mossakowski, V. Siffrin, T. Seelemann, H. Spiecker, I. Moll, J. Herz, A. E. Hauser, F. Zipp, M. J. Behne, and R. Niesner, “Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation,” PLoS One 8(4), e60100 (2013).
[Crossref] [PubMed]

Sheikine, Y.

Shi, T. T.

A. F. Tarantal, H. Chen, T. T. Shi, C. H. Lu, A. B. Fang, S. Buckley, M. Kolb, J. Gauldie, D. Warburton, and W. Shi, “Overexpression of transforming growth factor-beta1 in fetal monkey lung results in prenatal pulmonary fibrosis,” Eur. Respir. J. 36(4), 907–914 (2010).
[Crossref] [PubMed]

Shi, W.

A. F. Tarantal, H. Chen, T. T. Shi, C. H. Lu, A. B. Fang, S. Buckley, M. Kolb, J. Gauldie, D. Warburton, and W. Shi, “Overexpression of transforming growth factor-beta1 in fetal monkey lung results in prenatal pulmonary fibrosis,” Eur. Respir. J. 36(4), 907–914 (2010).
[Crossref] [PubMed]

Shrestha, S.

J. Park, P. Pande, S. Shrestha, F. Clubb, B. E. Applegate, and J. A. Jo, “Biochemical characterization of atherosclerotic plaques by endogenous multispectral fluorescence lifetime imaging microscopy,” Atherosclerosis 220(2), 394–401 (2012).
[Crossref] [PubMed]

Siffrin, V.

J. L. Rinnenthal, C. Börnchen, H. Radbruch, V. Andresen, A. Mossakowski, V. Siffrin, T. Seelemann, H. Spiecker, I. Moll, J. Herz, A. E. Hauser, F. Zipp, M. J. Behne, and R. Niesner, “Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation,” PLoS One 8(4), e60100 (2013).
[Crossref] [PubMed]

Simpson, G. J.

Song, J. W.

Southard, T. L.

I. Pavlova, K. R. Hume, S. A. Yazinski, J. Flanders, T. L. Southard, R. S. Weiss, and W. W. Webb, “Multiphoton microscopy and microspectroscopy for diagnostics of inflammatory and neoplastic lung,” J. Biomed. Opt. 17(3), 036014 (2012).
[Crossref] [PubMed]

Spiecker, H.

J. L. Rinnenthal, C. Börnchen, H. Radbruch, V. Andresen, A. Mossakowski, V. Siffrin, T. Seelemann, H. Spiecker, I. Moll, J. Herz, A. E. Hauser, F. Zipp, M. J. Behne, and R. Niesner, “Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation,” PLoS One 8(4), e60100 (2013).
[Crossref] [PubMed]

Squire, A.

P. I. H. Bastiaens and A. Squire, “Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell,” Trends Cell Biol. 9(2), 48–52 (1999).
[Crossref] [PubMed]

Stamp, G. W.

N. P. Galletly, J. McGinty, C. Dunsby, F. Teixeira, J. Requejo-Isidro, I. Munro, D. S. Elson, M. A. Neil, A. C. Chu, P. M. French, and G. W. Stamp, “Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin,” Br. J. Dermatol. 159(1), 152–161 (2008).
[Crossref] [PubMed]

Sullivan, S. Z.

Szmacinski, H.

J. R. Lakowicz, H. Szmacinski, K. Nowaczyk, and M. L. Johnson, “Fluorescence lifetime imaging of free and protein-bound NADH,” Proc. Natl. Acad. Sci. U.S.A. 89(4), 1271–1275 (1992).
[Crossref] [PubMed]

Tarantal, A. F.

A. F. Tarantal, H. Chen, T. T. Shi, C. H. Lu, A. B. Fang, S. Buckley, M. Kolb, J. Gauldie, D. Warburton, and W. Shi, “Overexpression of transforming growth factor-beta1 in fetal monkey lung results in prenatal pulmonary fibrosis,” Eur. Respir. J. 36(4), 907–914 (2010).
[Crossref] [PubMed]

Teixeira, F.

N. P. Galletly, J. McGinty, C. Dunsby, F. Teixeira, J. Requejo-Isidro, I. Munro, D. S. Elson, M. A. Neil, A. C. Chu, P. M. French, and G. W. Stamp, “Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin,” Br. J. Dermatol. 159(1), 152–161 (2008).
[Crossref] [PubMed]

Vardeh, H.

Wahl, M.

P. Kapusta, R. Erdmann, U. Ortmann, and M. Wahl, “Time-resolved fluorescence anisotropy measurements made simple,” J. Fluoresc. 13(2), 179–183 (2003).
[Crossref]

Warburton, D.

A. F. Tarantal, H. Chen, T. T. Shi, C. H. Lu, A. B. Fang, S. Buckley, M. Kolb, J. Gauldie, D. Warburton, and W. Shi, “Overexpression of transforming growth factor-beta1 in fetal monkey lung results in prenatal pulmonary fibrosis,” Eur. Respir. J. 36(4), 907–914 (2010).
[Crossref] [PubMed]

Webb, W. W.

I. Pavlova, K. R. Hume, S. A. Yazinski, J. Flanders, T. L. Southard, R. S. Weiss, and W. W. Webb, “Multiphoton microscopy and microspectroscopy for diagnostics of inflammatory and neoplastic lung,” J. Biomed. Opt. 17(3), 036014 (2012).
[Crossref] [PubMed]

Weiss, R. S.

I. Pavlova, K. R. Hume, S. A. Yazinski, J. Flanders, T. L. Southard, R. S. Weiss, and W. W. Webb, “Multiphoton microscopy and microspectroscopy for diagnostics of inflammatory and neoplastic lung,” J. Biomed. Opt. 17(3), 036014 (2012).
[Crossref] [PubMed]

Wiggins, R. C.

T. Hato, S. Winfree, R. Day, R. M. Sandoval, B. A. Molitoris, M. C. Yoder, R. C. Wiggins, Y. Zheng, K. W. Dunn, and P. C. Dagher, “Two-Photon Intravital Fluorescence Lifetime Imaging of the Kidney Reveals Cell-Type Specific Metabolic Signatures,” J. Am. Soc. Nephrol. 28(8), 2420–2430 (2017).
[Crossref] [PubMed]

Winfree, S.

T. Hato, S. Winfree, R. Day, R. M. Sandoval, B. A. Molitoris, M. C. Yoder, R. C. Wiggins, Y. Zheng, K. W. Dunn, and P. C. Dagher, “Two-Photon Intravital Fluorescence Lifetime Imaging of the Kidney Reveals Cell-Type Specific Metabolic Signatures,” J. Am. Soc. Nephrol. 28(8), 2420–2430 (2017).
[Crossref] [PubMed]

Won, Y.

Won, Y. J.

Wright, J.

Wright, J. M.

B. H. Malik, J. Lee, S. Cheng, R. Cuenca, J. M. Jabbour, Y. S. L. Cheng, J. M. Wright, B. Ahmed, K. C. Maitland, and J. A. Jo, “Objective Detection of Oral Carcinoma with Multispectral Fluorescence Lifetime Imaging In Vivo,” Photochem. Photobiol. 92(5), 694–701 (2016).
[Crossref] [PubMed]

Yang, W.

Yazinski, S. A.

I. Pavlova, K. R. Hume, S. A. Yazinski, J. Flanders, T. L. Southard, R. S. Weiss, and W. W. Webb, “Multiphoton microscopy and microspectroscopy for diagnostics of inflammatory and neoplastic lung,” J. Biomed. Opt. 17(3), 036014 (2012).
[Crossref] [PubMed]

Yoder, M. C.

T. Hato, S. Winfree, R. Day, R. M. Sandoval, B. A. Molitoris, M. C. Yoder, R. C. Wiggins, Y. Zheng, K. W. Dunn, and P. C. Dagher, “Two-Photon Intravital Fluorescence Lifetime Imaging of the Kidney Reveals Cell-Type Specific Metabolic Signatures,” J. Am. Soc. Nephrol. 28(8), 2420–2430 (2017).
[Crossref] [PubMed]

Yoo, H.

Zheng, Y.

T. Hato, S. Winfree, R. Day, R. M. Sandoval, B. A. Molitoris, M. C. Yoder, R. C. Wiggins, Y. Zheng, K. W. Dunn, and P. C. Dagher, “Two-Photon Intravital Fluorescence Lifetime Imaging of the Kidney Reveals Cell-Type Specific Metabolic Signatures,” J. Am. Soc. Nephrol. 28(8), 2420–2430 (2017).
[Crossref] [PubMed]

Zipp, F.

J. L. Rinnenthal, C. Börnchen, H. Radbruch, V. Andresen, A. Mossakowski, V. Siffrin, T. Seelemann, H. Spiecker, I. Moll, J. Herz, A. E. Hauser, F. Zipp, M. J. Behne, and R. Niesner, “Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation,” PLoS One 8(4), e60100 (2013).
[Crossref] [PubMed]

Zvyagin, A. V.

Y. Dancik, A. Favre, C. J. Loy, A. V. Zvyagin, and M. S. Roberts, “Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentation in vivo,” J. Biomed. Opt. 18(2), 026022 (2013).
[Crossref] [PubMed]

Appl. Opt. (1)

Applied Microscopy (1)

J. Kim, J. Ryu, and D. Gweon, “Real-time Fluorescence Lifetime Imaging Microscopy Implementation by Analog Mean-Delay Method through Parallel Data Processing,” Applied Microscopy 46(1), 6–13 (2016).
[Crossref]

Atherosclerosis (1)

J. Park, P. Pande, S. Shrestha, F. Clubb, B. E. Applegate, and J. A. Jo, “Biochemical characterization of atherosclerotic plaques by endogenous multispectral fluorescence lifetime imaging microscopy,” Atherosclerosis 220(2), 394–401 (2012).
[Crossref] [PubMed]

Biomed. Opt. Express (5)

Br. J. Dermatol. (1)

N. P. Galletly, J. McGinty, C. Dunsby, F. Teixeira, J. Requejo-Isidro, I. Munro, D. S. Elson, M. A. Neil, A. C. Chu, P. M. French, and G. W. Stamp, “Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin,” Br. J. Dermatol. 159(1), 152–161 (2008).
[Crossref] [PubMed]

Chem. Rev. (1)

M. Y. Berezin and S. Achilefu, “Fluorescence Lifetime Measurements and Biological Imaging,” Chem. Rev. 110(5), 2641–2684 (2010).
[Crossref] [PubMed]

Eur. Respir. J. (1)

A. F. Tarantal, H. Chen, T. T. Shi, C. H. Lu, A. B. Fang, S. Buckley, M. Kolb, J. Gauldie, D. Warburton, and W. Shi, “Overexpression of transforming growth factor-beta1 in fetal monkey lung results in prenatal pulmonary fibrosis,” Eur. Respir. J. 36(4), 907–914 (2010).
[Crossref] [PubMed]

IEEE J. Sel. Top. Quantum Electron (1)

C. Li, C. Pitsillides, J. M. Runnels, D. Côté, and C. P. Lin, “Multiphoton Microscopy of Live Tissues With Ultraviolet Autofluorescence,” IEEE J. Sel. Top. Quantum Electron 16(3), 516–523 (2010).
[Crossref]

Int. J. Mol. Sci. (1)

M. S. Islam, M. Honma, T. Nakabayashi, M. Kinjo, and N. Ohta, “pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells,” Int. J. Mol. Sci. 14(1), 1952–1963 (2013).
[Crossref] [PubMed]

J. Am. Soc. Nephrol. (1)

T. Hato, S. Winfree, R. Day, R. M. Sandoval, B. A. Molitoris, M. C. Yoder, R. C. Wiggins, Y. Zheng, K. W. Dunn, and P. C. Dagher, “Two-Photon Intravital Fluorescence Lifetime Imaging of the Kidney Reveals Cell-Type Specific Metabolic Signatures,” J. Am. Soc. Nephrol. 28(8), 2420–2430 (2017).
[Crossref] [PubMed]

J. Biomed. Opt. (3)

I. Pavlova, K. R. Hume, S. A. Yazinski, J. Flanders, T. L. Southard, R. S. Weiss, and W. W. Webb, “Multiphoton microscopy and microspectroscopy for diagnostics of inflammatory and neoplastic lung,” J. Biomed. Opt. 17(3), 036014 (2012).
[Crossref] [PubMed]

Y. Dancik, A. Favre, C. J. Loy, A. V. Zvyagin, and M. S. Roberts, “Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentation in vivo,” J. Biomed. Opt. 18(2), 026022 (2013).
[Crossref] [PubMed]

J. M. Jabbour, S. Cheng, B. H. Malik, R. Cuenca, J. A. Jo, J. Wright, Y. S. L. Cheng, and K. C. Maitland, “Fluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer,” J. Biomed. Opt. 18(4), 046012 (2013).
[Crossref] [PubMed]

J. Fluoresc. (2)

P. Kapusta, R. Erdmann, U. Ortmann, and M. Wahl, “Time-resolved fluorescence anisotropy measurements made simple,” J. Fluoresc. 13(2), 179–183 (2003).
[Crossref]

A. S. Kristoffersen, S. R. Erga, B. Hamre, and Ø. Frette, “Testing Fluorescence Lifetime Standards using Two-Photon Excitation and Time-Domain Instrumentation: Rhodamine B, Coumarin 6 and Lucifer Yellow,” J. Fluoresc. 24(4), 1015–1024 (2014).
[Crossref] [PubMed]

J. Microsc. (1)

W. Becker, “Fluorescence lifetime imaging--techniques and applications,” J. Microsc. 247(2), 119–136 (2012).
[Crossref] [PubMed]

J. Opt. Soc. Am. A (2)

Nature (2)

D. Bray, “Protein Molecules as Computational Elements in Living Cells,” Nature 376(6538), 307–312 (1995).
[Crossref] [PubMed]

N. Barkai and S. Leibler, “Robustness in simple biochemical networks,” Nature 387(6636), 913–917 (1997).
[Crossref] [PubMed]

Opt. Express (2)

Opt. Lett. (2)

Photochem. Photobiol. (1)

B. H. Malik, J. Lee, S. Cheng, R. Cuenca, J. M. Jabbour, Y. S. L. Cheng, J. M. Wright, B. Ahmed, K. C. Maitland, and J. A. Jo, “Objective Detection of Oral Carcinoma with Multispectral Fluorescence Lifetime Imaging In Vivo,” Photochem. Photobiol. 92(5), 694–701 (2016).
[Crossref] [PubMed]

PLoS One (1)

J. L. Rinnenthal, C. Börnchen, H. Radbruch, V. Andresen, A. Mossakowski, V. Siffrin, T. Seelemann, H. Spiecker, I. Moll, J. Herz, A. E. Hauser, F. Zipp, M. J. Behne, and R. Niesner, “Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation,” PLoS One 8(4), e60100 (2013).
[Crossref] [PubMed]

Proc. Natl. Acad. Sci. U.S.A. (1)

J. R. Lakowicz, H. Szmacinski, K. Nowaczyk, and M. L. Johnson, “Fluorescence lifetime imaging of free and protein-bound NADH,” Proc. Natl. Acad. Sci. U.S.A. 89(4), 1271–1275 (1992).
[Crossref] [PubMed]

Respir. Res. (1)

H. Fehrenbach, “Alveolar epithelial type II cell: defender of the alveolus revisited,” Respir. Res. 2(1), 33–46 (2001).
[Crossref] [PubMed]

Trends Cell Biol. (1)

P. I. H. Bastiaens and A. Squire, “Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell,” Trends Cell Biol. 9(2), 48–52 (1999).
[Crossref] [PubMed]

Other (2)

W. Becker, Advanced Time-Correlated Single Photon Counting Applications (Springer International Publishing, 2015).

Laser Institute of America, “National Standard for Safe Use of Lasers ANSI Z136.1-2000,” (American National Standards Institute, Inc., 2000).

Supplementary Material (1)

NameDescription
» Visualization 1       A video that captured the moment of lung tissue imaging. It visualizes robust lifetime value in real-time. (the range of the lifetime color-bar in the video is 0 to 5 ns).

Cited By

OSA participates in Crossref's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (7)

Fig. 1
Fig. 1 (a) System schematic and (b) process flow diagram of high-speed two-photon fluorescence lifetime imaging microscope (TP-FLIM) (AO: Analog output, DI: Digital input).
Fig. 2
Fig. 2 An example of timing deviation between the laser sync (red curve) and the sample point of the digitizer (dotted black vertical line) in case the pulse repetition rate is 76 MHz, signal (gray curve): (a) initial state, (b) after applying the sample skipping algorithm, and (c) the relationship between the pulse repetition rate and the number of sample points (N), every Nth sample point tdev becomes larger than 1 ns.
Fig. 3
Fig. 3 (a) The pulse repetition rate (PRR) and its corresponding t′devlast value: for an example a PRR range that gives local minimum value is enlarge at the right side of the graph (red box), (b) a look-up table of the available PRRs.
Fig. 4
Fig. 4 FLIM image obtained by the reflected signal from a mirror surface for calibration and the bottom graph represents the lifetime value along the dotted line marked within the image: (a) initial image before any error correction, (b) image after skipping sample points when tdev becomes larger than 1 ns, and (c) final image after numerical error correction.
Fig. 5
Fig. 5 (a) Wide-field fluorescence images and (b) TP-FLIM images of stained BPAEC. The number of averaging and the imaging time are written within each image.
Fig. 6
Fig. 6 Mouse kidney tissue: (a) cross-sectional images of TPM and FLIM along z-axis, (b) cross-sectional image at XY, XZ, and YZ plane, and (c) 3D rendering.
Fig. 7
Fig. 7 The lung tissue images of 5-weeks old healthy mouse: (a) FLIM images taken at the cutting plane, (b) composite images of TPM and SHG, and (c) FLIM images taken at the surface area of the lung tissue.

Equations (1)

Equations on this page are rendered with MathJax. Learn more.

τ= τ fl τ irf = t i fl ( t )dt i fl ( t )dt t i irf ( t )dt i irf ( t )dt

Metrics