Abstract

The standard two-dimensional (2D) image recorded in bright-field fluorescence microscopy is rigorously modeled by a convolution process involving a three-dimensional (3D) sample and a 3D point spread function. We show on synthetic and experimental data that deconvolving the 2D image using the appropriate 3D point spread function reduces the contribution of the out-of-focus fluorescence, resulting in a better image contrast and resolution. This approach is particularly interesting for superresolution speckle microscopy, in which the resolution gain stems directly from the efficiency of the deconvolution of each speckle image.

© 2019 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Improving the axial and lateral resolution of three-dimensional fluorescence microscopy using random speckle illuminations

Awoke Negash, Simon Labouesse, Nicolas Sandeau, Marc Allain, Hugues Giovannini, Jérôme Idier, Rainer Heintzmann, Patrick C. Chaumet, Kamal Belkebir, and Anne Sentenac
J. Opt. Soc. Am. A 33(6) 1089-1094 (2016)

Two-photon speckle illumination for super-resolution microscopy

Awoke Negash, Simon Labouesse, Patrick C. Chaumet, Kamal Belkebir, Hugues Giovannini, Marc Allain, Jérôme Idier, and Anne Sentenac
J. Opt. Soc. Am. A 35(6) 1028-1033 (2018)

Super-resolution algorithm based on Richardson–Lucy deconvolution for three-dimensional structured illumination microscopy

Yanwei Zhang, Song Lang, Hongwei Wang, Jiasheng Liao, and Yan Gong
J. Opt. Soc. Am. A 36(2) 173-178 (2019)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (6)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription