Abstract

A new laser scanning microscope is proposed for imaging a three-dimensional absorption structure of a sample. The microscope is based on a confocal system built with excitation and absorption-monitoring lasers. The excitation laser produces excited molecules, and the absorption-monitoring laser probes their transient absorption. A three-dimensional optical transfer function of the microscope is theoretically derived to demonstrate a tomographic imaging capability. The spatial frequency cutoff is confirmed to be independent of the detector size. An experimental result is shown to verify the depth discrimination property.

© 1992 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Three-dimensional transfer-function analysis of the tomographic capability of a confocal fluorescence microscope

Osamu Nakamura and Satoshi Kawata
J. Opt. Soc. Am. A 7(3) 522-526 (1990)

Quantitative comparison of background rejection, signal-to-noise ratio, and resolution in confocal and full-field laser scanning microscopes

D. R. Sandison, D. W. Piston, R. M. Williams, and W. W. Webb
Appl. Opt. 34(19) 3576-3588 (1995)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (18)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription