Abstract

We theoretically demonstrate a technique allowing a highly efficient observation of all single-molecule emitters in one fluorescent image, regardless of the orientations of their transition dipole moments. To perform this technique, we combine near-unity collection efficiency for an arbitrarily oriented emitter and the elimination of the excitation efficiency dependence on the emitter’s orientation. The former task is solved by placing the emitter in a special metallo-dielectric antenna [Opt. Lett. 36, 3545 (2011) [CrossRef]  ]; the latter, by means of laser-scanning confocal fluorescence microscopy using elliptically polarized cylindrical vector beams [J. Exp. Theor. Phys. Lett. 97, 52 (2013) [CrossRef]  ]. We present conditions under which the collection efficiency for arbitrarily oriented emitters is >98% and the excitation efficiency of an emitter of minimally excitable orientation amounts to >92% of the excitation efficiency of an emitter of maximally excitable orientation. These conditions are satisfied in the broad spectral range between 0.4 and 1.0 μm: the emitters can be excited with any wavelength and emit any fluorescence spectra in this range. The presented results are based on simulation data.

© 2015 Optical Society of America

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