Abstract

A portable fluorescence cytometric system has been developed for characterizing chemical concentration and cellular status in microscale cell culture analog (μCCA) devices. Based on discrete optical components, the system provides a modular platform for real-time image measurements applicable to a variety of cell-based microassays. As a feasibility study, we investigated the real-time dynamics of daunorubicin uptake with cultured mouse L-cells in a μCCA compartment. Time course results measured by the portable fluorescence cytometric system confirmed that in the μCCA devices daunorubicin accumulation is proportional to the liquid turnover rate.

© 2005 Optical Society of America

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