Background-free 3D chemical imaging based on polarization coherent Raman holography

Abstract

We report on a holographic coherent anti-Stokes Raman scattering imaging by using polarization discrimination for nonresonant background suppression. With reduced polarization scrambling effect under weakly focused excitation, nonresonant background-free coherent Raman imaging is demonstrated. A fast chemically selective imaging is achieved in a three-dimensional volume of $70\mathrm{\mu m}\times 70\mathrm{\mu m}\times 100\mathrm{\mu m}$ in 2 s.

© 2015 Optical Society of America

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