Abstract

We performed noninvasive video imaging of retinal blood flow in a pigmented rat by holographic interferometry of near-infrared laser light backscattered by retinal tissue, beating against an off-axis reference beam sampled at a frame rate of 39 kHz with a high throughput camera. Local Doppler contrasts emerged from the envelopes of short-time Fourier transforms and the phase of autocorrelation functions of holograms rendered by Fresnel transformation. This approach permitted imaging of blood flow in large retinal vessels (30 microns diameter) over 400×400  pixels with a spatial resolution of 8 microns and a temporal resolution of 6.5  ms.

© 2016 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
In vivo dynamic human retinal blood flow imaging using ultra-high-speed spectral domain optical Doppler tomography

Brian R. White, Mark C. Pierce, Nader Nassif, Barry Cense, B. Hyle Park, Guillermo J. Tearney, Brett E. Bouma, Teresa C. Chen, and Johannes F. de Boer
Opt. Express 11(25) 3490-3497 (2003)

Waveform analysis of human retinal and choroidal blood flow with laser Doppler holography

Léo Puyo, Michel Paques, Mathias Fink, José-Alain Sahel, and Michael Atlan
Biomed. Opt. Express 10(10) 4942-4963 (2019)

Volumetric and quantitative imaging of retinal blood flow in rats with optical microangiography

Zhongwei Zhi, William Cepurna, Elaine Johnson, Tueng Shen, John Morrison, and Ruikang K Wang
Biomed. Opt. Express 2(3) 579-591 (2011)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Supplementary Material (6)

NameDescription
» Visualization 1: MOV (7503 KB)      Zeroth moment of the spectrum M0.
» Visualization 2: MOV (9664 KB)      First moment of the spectrum M1.
» Visualization 3: MOV (13503 KB)      Second moment of the spectrum M2.
» Visualization 4: MOV (22816 KB)      Composite image M0 M2.
» Visualization 5: MOV (28682 KB)      Composite image M0 M1.
» Visualization 6: MOV (4420 KB)      Phase images.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (4)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription