Abstract

High-resolution nonlinear fluorescence (NF) microscopy that utilizes repetitive stimulated transition due to the saturation of stimulated emission caused by two-color continuous-wave lasers was developed. The resulting NF signal, detected via the lock-in technique, is produced by the multiplicative combination of incident beams, which results in an improvement of the optical resolution. The proposed method is demonstrated to have a three-dimensional optical resolution superior to that of conventional NF microscopy. The results of biological imaging reveal the feasibility and superiority of the proposed method.

© 2019 Optical Society of America

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