Abstract

Detailed assessment of skin conditions or the efficacy of skin treatments could greatly benefit from noninvasively assessing the distribution of cutaneous and subcutaneous structures and biomolecules. We considered ultrawideband raster scan optoacoustic mesoscopy with an extended wavelength range from visible to short-wave infrared and observed previously unseen high-resolution images of lipids colocalized with water, melanin, and hemoglobin distribution in human skin. Based on this contrast, the technique resolves subcutaneous fat, the pilosebaceous unit with complete hair strand and bulb, dermal microvasculature, and epidermal structures. We further visualize melanoidins that form via the Maillard reaction in the ultrathin stratum corneum layer, analyze their absorption spectrum, and separate them from the melanin layer. The suggested method may allow novel interrogation of skin conditions, possibly impacting diagnostics and medical and cosmetic treatments.

© 2019 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Potential of short-wave infrared spectroscopy for quantitative depth profiling of stratum corneum lipids and water in dermatology

Anna Ezerskaia, Natallia Eduarda Uzunbajakava, Gerwin J. Puppels, Johanna de Sterke, Peter J. Caspers, H. Paul Urbach, and Babu Varghese
Biomed. Opt. Express 9(5) 2436-2450 (2018)

Optical mesoscopy without the scatter: broadband multispectral optoacoustic mesoscopy

Andrei Chekkoury, Jérôme Gateau, Wouter Driessen, Panagiotis Symvoulidis, Nicolas Bézière, Annette Feuchtinger, Axel Walch, and Vasilis Ntziachristos
Biomed. Opt. Express 6(9) 3134-3148 (2015)

Automatic characterization and segmentation of human skin using three-dimensional optical coherence tomography

Yasuaki Hori, Yoshiaki Yasuno, Shingo Sakai, Masayuki Matsumoto, Tomoko Sugawara, Violeta Dimitrova Madjarova, Masahiro Yamanari, Shuichi Makita, Takeshi Yasui, Tsutomu Araki, Masahide Itoh, and Toyohiko Yatagai
Opt. Express 14(5) 1862-1877 (2006)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Supplementary Material (5)

NameDescription
» Visualization 1       3D optoacoustic image of human skin using UWSB-RSOM. Distribution of hemoglobin (microvasculature) and melanin in skin at 515 nm.
» Visualization 2       3D optoacoustic image of human skin using UWSB-RSOM. Distribution of hemoglobin (microvasculature) and melanin in skin at 532 nm.
» Visualization 3       3D optoacoustic image of human skin using UWSB-RSOM. Melanin in epidermis and hair imaged at 650 nm.
» Visualization 4       3D optoacoustic image of human skin using UWSB-RSOM. Distribution of lipids and water in skin at 1210 nm.
» Visualization 5       3D optoacoustic image of human skin using UWSB-RSOM. Distribution of water in skin at 1450 nm.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription