July 2019
Spotlight Summary by Pavan Chandra Konda
Speckle-structured illumination for 3D phase and fluorescence computational microscopy
Drug discovery requires imaging large volumes of synthetic tissues and cell cultures to study their drug interactions and improve the drug formula. The commercial microscopes currently used for imaging are expensive and can only image small areas at the required high-resolution, limiting the number of parallel experiments. Computational imaging techniques have been recently proposed to solve this for imaging thin samples; however, it is still a challenging problem for imaging thick samples such as tissue sections and dense cell cultures. Here, Li-Hao and colleagues present an exciting approach for imaging these thick samples concurrently in transmission and fluorescence imaging modalities.
Unlike traditional methods, a laser speckle is used here to illuminate the sample. A speckle is a random grainy pattern in both 2D and 3D, resulting from a laser scattered by a rough object, in this case a Scotch tape. This speckle pattern is scanned across the object to provide diversity in the recorded measurements and enable high-resolution 3D imaging across a wide field-of-view using a low-resolution objective. The resolution of this system is only limited by the speckle grain size, which can be easily made smaller using thicker Scotch tape. HT-29 cancer cells across a 314×500×24 µm3 volume were successfully imaged at 0.6×0.6×6 µm3 voxel resolution to show the potential for this technique as a low-cost solution for imaging 3D samples.
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Unlike traditional methods, a laser speckle is used here to illuminate the sample. A speckle is a random grainy pattern in both 2D and 3D, resulting from a laser scattered by a rough object, in this case a Scotch tape. This speckle pattern is scanned across the object to provide diversity in the recorded measurements and enable high-resolution 3D imaging across a wide field-of-view using a low-resolution objective. The resolution of this system is only limited by the speckle grain size, which can be easily made smaller using thicker Scotch tape. HT-29 cancer cells across a 314×500×24 µm3 volume were successfully imaged at 0.6×0.6×6 µm3 voxel resolution to show the potential for this technique as a low-cost solution for imaging 3D samples.
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Jessica L.
08/29/2019 7:10 AM
Thanks for the great article. You have done a great job!
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