Live fluorescence imaging/microscopy is one of the essential tools in the contemporary biomedicine arsenal. At the same time, the detrimental consequences of phototoxicity, which often occurs during live imaging/microscopy, are usually underestimated. This issue becomes most important when a high energy laser with emission in the visible wavelength range is employed as excitation source in stimulated emission depletion microscopy. The shift of laser emission to the red wing of the visible range reduces the photodamage in biological tissue and live cells considerably. However, the availability of pulsed red sources, which meet specific requirements for imaging techniques, is limited.
The authors of this paper, recently published in Optics Letters
, demonstrated a nanosecond pulsed source at 620 nm with watt-level average power by frequency-doubling pulses from a 1240-nm phosphosilicate Raman fiber amplifier. The proposed design with the emission wavelength bandwidth <0.1 nm, pulse repetition rate of 5 MHz, pulse duration of ~1 ns, and beam quality M2
<1.16 can fulfill the need for reliable red lasers in biomedical high-resolution microscopy.
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